Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,637	T→C	intergenic (‑6/+13)	ygdD ← / ← gcvA	conserved inner membrane protein YgdD/DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,637	0	T	C	100.0%	140.4 / NA	42	intergenic (‑6/+13)	ygdD/gcvA	conserved inner membrane protein YgdD/DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/23);  total (19/23)

AGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAG  >  NC_000913/2941501‑2941763                                                                                                                                         |                                                                                                                               aGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCAcc                                                                                                                             <  1:1162013/140‑1 (MQ=255)     cGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTAc                                                                                                                         <  1:699491/140‑1 (MQ=255)     cGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTAc                                                                                                                         >  2:841298/1‑140 (MQ=255)       gTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGt                                                                                                                       >  1:278483/1‑140 (MQ=255)       gTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGt                                                                                                                       >  1:345352/1‑140 (MQ=255)        tCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTa                                                                                                                      <  2:278483/140‑1 (MQ=255)          tGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTaaa                                                                                                                    <  1:841298/140‑1 (MQ=255)                  gCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGtt                                                                                                            >  1:317051/1‑140 (MQ=255)                      aTCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATa                                                                                                        >  2:518502/1‑140 (MQ=255)                      aTCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATa                                                                                                        >  1:550439/1‑140 (MQ=255)                      aTCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATa                                                                                                        >  2:877135/1‑140 (MQ=255)                             cGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAatta                                                                                                                 >  2:162937/1‑124 (MQ=255)                             cGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAatta                                                                                                                 <  1:162937/124‑1 (MQ=255)                                  cccATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGaa                                                                                            >  1:602624/1‑140 (MQ=255)                                   ccATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAAt                                                                                           >  2:1190907/1‑140 (MQ=255)                                            tACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGt                                                                                  >  2:200480/1‑140 (MQ=255)                                                   cacaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagcag                                                                           >  2:933754/1‑140 (MQ=255)                                                   cacaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagcag                                                                           <  2:317051/140‑1 (MQ=255)                                                   cacaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagcag                                                                           >  2:109863/1‑140 (MQ=255)                                                   cacaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagcag                                                                           >  2:667006/1‑140 (MQ=255)                                                        gcgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCt                                                                      >  1:481112/1‑140 (MQ=255)                                                         cgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCtt                                                                     >  2:908816/1‑140 (MQ=255)                                                           cgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTc                                                                   >  2:555098/1‑140 (MQ=255)                                                            gcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCg                                                                  <  2:1128189/140‑1 (MQ=255)                                                             ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCtt                                                                     <  1:942371/136‑1 (MQ=255)                                                             ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCtt                                                                     >  2:942371/1‑136 (MQ=255)                                                             ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGc                                                                 <  2:1100635/140‑1 (MQ=255)                                                              cAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGcc                                                                <  2:550439/140‑1 (MQ=255)                                                               aaaaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCa                                                               <  1:640053/140‑1 (MQ=255)                                                               aaaaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCa                                                               <  2:602624/140‑1 (MQ=255)                                                               aaaaGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCa                                                               <  1:109863/140‑1 (MQ=255)                                                                                caAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTgg                                                    >  2:1170043/1‑134 (MQ=255)                                                                                caAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTgg                                                    <  1:1170043/134‑1 (MQ=255)                                                                                   aaaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAggcg                                           <  2:133058/140‑1 (MQ=255)                                                                                         aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                     <  1:933754/140‑1 (MQ=255)                                                                                         aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                     <  1:908816/140‑1 (MQ=255)                                                                                             gCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTAc                                 <  2:481112/140‑1 (MQ=255)                                                                                                 aTGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAg                             <  1:555098/140‑1 (MQ=255)                                                                                                        cgAAAATCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGcc                      <  1:853967/140‑1 (MQ=255)                                                                                                          aaaaTCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTg                    <  1:877135/140‑1 (MQ=255)                                                                                                           aaaTCAGCATAAAACGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGa                   <  1:1153143/140‑1 (MQ=255)                                                                                                                     aaaaCGGCTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGAc         <  1:1190907/140‑1 (MQ=255)                                                                                                                            cTGGTCATGGTCGCACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAg  >  1:958751/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               AGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAG  >  NC_000913/2941501‑2941763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑