Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,159	G→A	G58D (GGC→GAC)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,159	0	G	A	100.0%	287.6 / NA	82	G58D (GGC→GAC)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/45);  total (37/45)

CTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGGCGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCG  >  NC_000913/3063025‑3063293                                                                                                                                       |                                                                                                                                       cTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTa                                                                                                                                   <  1:899512/140‑1 (MQ=255)   tACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTAcc                                                                                                                                 <  2:878811/140‑1 (MQ=255)   tACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTAcc                                                                                                                                 <  2:1103450/140‑1 (MQ=255)     cGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCgg                                                                                                                               <  1:812872/140‑1 (MQ=255)               aGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGgc                                                                                                                     >  1:376568/1‑140 (MQ=255)               aGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGgc                                                                                                                     >  2:177762/1‑140 (MQ=255)               aGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGgc                                                                                                                     >  1:263866/1‑140 (MQ=255)                gCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCgg                                                                                                                               >  2:188395/1‑129 (MQ=255)                gCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCgg                                                                                                                               <  1:188395/129‑1 (MQ=255)                gCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGgcg                                                                                                                    <  1:863213/140‑1 (MQ=255)                gCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGgcg                                                                                                                    >  2:519551/1‑140 (MQ=255)                  gTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGcgcg                                                                                                                  <  1:519551/140‑1 (MQ=255)                   tGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCgg                                                                                                                 >  1:111567/1‑140 (MQ=255)                    gCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCggg                                                                                                                >  1:887229/1‑140 (MQ=255)                     ccACACTCGCCCAGGCCATGACGCTGGTGGAAGGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCgggg                                                                                                               >  2:1058897/1‑140 (MQ=255)                       acacTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGaa                                                                                                             <  2:1062455/140‑1 (MQ=255)                            cGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTa                                                                                                        <  1:46655/140‑1 (MQ=255)                             gCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTAc                                                                                                       <  2:263866/140‑1 (MQ=255)                               ccAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCt                                                                                                     <  1:166998/140‑1 (MQ=255)                                   gCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCAGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCt                                                                                                 >  2:354580/1‑140 (MQ=255)                                     cATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTg                                                                                               <  2:536115/140‑1 (MQ=255)                                      aTGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGa                                                                                              <  2:645395/140‑1 (MQ=255)                                         aCGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGc                                                                                           >  1:288536/1‑140 (MQ=255)                                           gCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCt                                                                                         <  2:595363/140‑1 (MQ=255)                                           gCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCt                                                                                         <  1:356095/140‑1 (MQ=255)                                              ggtggAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTg                                                                                      <  2:144323/140‑1 (MQ=255)                                                tggAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGc                                                                                    <  2:205186/140‑1 (MQ=255)                                                tggAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGc                                                                                    <  2:1105848/140‑1 (MQ=255)                                                 ggAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCa                                                                                   <  1:177762/140‑1 (MQ=255)                                                 ggAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCa                                                                                   <  1:905322/140‑1 (MQ=255)                                                       ccGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCAtgttgt                                                                             <  2:887229/140‑1 (MQ=255)                                                         gTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCa                                                                                   <  2:352201/132‑1 (MQ=255)                                                         gTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCa                                                                                   >  1:352201/1‑132 (MQ=255)                                                         gTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGttgttg                                                                           >  1:1093865/1‑140 (MQ=255)                                                         gTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGttgttg                                                                           >  1:1041887/1‑140 (MQ=255)                                                          tCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGa                                                                          >  1:279683/1‑140 (MQ=255)                                                          tCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGa                                                                          >  1:892539/1‑140 (MQ=255)                                                           cACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGAt                                                                         <  2:1079862/140‑1 (MQ=255)                                                               cgcgTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCga                                                                     <  2:288536/140‑1 (MQ=255)                                                                gcgTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCgag                                                                    <  1:241475/140‑1 (MQ=255)                                                                 cgTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGAGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCgaga                                                                   <  1:736934/140‑1 (MQ=255)                                                                  gTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGTCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGagag                                                                  <  2:1093865/140‑1 (MQ=255)                                                                        aGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTa                                                            >  2:892519/1‑140 (MQ=255)                                                                        aGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTa                                                            >  1:1033297/1‑140 (MQ=255)                                                                            aCTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGagag                                                                  >  2:648394/1‑130 (MQ=255)                                                                            aCTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGagag                                                                  <  1:648394/130‑1 (MQ=255)                                                                             cTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGt                                                       <  2:1041887/140‑1 (MQ=255)                                                                             cTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGt                                                       <  2:892539/140‑1 (MQ=255)                                                                             cTAAGTACGCAGCAGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGt                                                       <  1:354580/140‑1 (MQ=255)                                                                              tAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTc                                                      <  2:279683/140‑1 (MQ=255)                                                                                    cGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGtt                                                >  1:88731/1‑140 (MQ=255)                                                                                         ctgctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGc                                           >  1:558295/1‑140 (MQ=255)                                                                                          tgctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCg                                          >  1:808386/1‑140 (MQ=255)                                                                                          tgctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCg                                          <  1:761987/140‑1 (MQ=255)                                                                                          tgctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCg                                          >  2:761987/1‑140 (MQ=255)                                                                                            ctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGt                                        >  1:793401/1‑140 (MQ=255)                                                                                            ctTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGt                                        >  1:377287/1‑140 (MQ=255)                                                                                               gATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGa                                     <  1:355476/140‑1 (MQ=255)                                                                                                      ttATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCgg                                         >  2:1048004/1‑129 (MQ=255)                                                                                                      ttATGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCgg                                         <  1:1048004/129‑1 (MQ=255)                                                                                                         tGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCgg                                         <  2:992158/126‑1 (MQ=255)                                                                                                         tGCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCgg                                         >  1:992158/1‑126 (MQ=255)                                                                                                          gCCGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGcc                          <  2:1033297/140‑1 (MQ=255)                                                                                                           ccGTACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGccc                         >  1:799480/1‑140 (MQ=255)                                                                                                              tACTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGt                      >  2:883110/1‑140 (MQ=255)                                                                                                                cTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCa                    >  2:1141186/1‑140 (MQ=255)                                                                                                                cTGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCa                    >  2:731564/1‑140 (MQ=255)                                                                                                                 tGCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCAc                   <  2:808386/140‑1 (MQ=255)                                                                                                                  gCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACt                  <  2:111567/140‑1 (MQ=255)                                                                                                                  gCGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACt                  <  2:558295/140‑1 (MQ=255)                                                                                                                   cGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTg                 >  1:1092852/1‑140 (MQ=255)                                                                                                                   cGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTg                 >  1:530809/1‑140 (MQ=255)                                                                                                                   cGGTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTg                 >  2:164367/1‑140 (MQ=255)                                                                                                                     gTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGc               >  2:770773/1‑140 (MQ=255)                                                                                                                     gTAACACCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGc               >  1:1091083/1‑140 (MQ=255)                                                                                                                        acacCCTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGt            <  1:1058897/140‑1 (MQ=255)                                                                                                                            ccTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCa        <  2:646935/140‑1 (MQ=255)                                                                                                                             cTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCa        >  2:1014839/1‑139 (MQ=255)                                                                                                                             cTGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCa        <  1:1014839/139‑1 (MQ=255)                                                                                                                              tGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCg              <  2:343199/132‑1 (MQ=255)                                                                                                                              tGCGACTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCg              >  1:343199/1‑132 (MQ=255)                                                                                                                                  aCTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCg  <  1:731564/140‑1 (MQ=255)                                                                                                                                  aCTGGACGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCg  <  2:405454/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGGCGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCG  >  NC_000913/3063025‑3063293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑