Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,073,789	G→A	intergenic (‑98/+187)	epd ← / ← yggC	D‑erythrose‑4‑phosphate dehydrogenase/P‑loop NTPase domain‑containing protein YggC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,073,789	0	G	A	100.0%	280.4 / NA	78	intergenic (‑98/+187)	epd/yggC	D‑erythrose‑4‑phosphate dehydrogenase/P‑loop NTPase domain‑containing protein YggC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/37);  total (41/37)

TGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTGGGAAGC  >  NC_000913/3073655‑3073925                                                                                                                                        |                                                                                                                                         tGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTaa                                                                                                                                     <  2:411992/140‑1 (MQ=255)      ccGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTc                                                                                                                                <  1:132397/140‑1 (MQ=255)           gCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACa                                                                                                                           >  1:225071/1‑140 (MQ=255)                ttATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTaa                                                                                                                      >  2:956611/1‑140 (MQ=255)                      cTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACt                                                                                                                >  1:1144952/1‑140 (MQ=255)                       tACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTg                                                                                                               <  2:661605/140‑1 (MQ=255)                        aCGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGa                                                                                                              >  2:290585/1‑140 (MQ=255)                         cgcgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAAt                                                                                                                     <  1:563389/132‑1 (MQ=255)                         cgcgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAAt                                                                                                                     >  2:563389/1‑132 (MQ=255)                         cgcgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGaa                                                                                                             >  1:71373/1‑140 (MQ=255)                           cgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAAc                                                                                                           >  2:1177020/1‑140 (MQ=255)                              aCGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  1:534597/140‑1 (MQ=255)                              aCGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  2:71373/140‑1 (MQ=255)                              aCGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  1:224690/140‑1 (MQ=255)                               cGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCtt                                                                                                       <  2:556047/140‑1 (MQ=255)                               cGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCtt                                                                                                       <  2:998774/140‑1 (MQ=255)                               cGGTCATAGATCTCCTGCAAGGTTTCCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCtt                                                                                                       <  1:1129595/140‑1 (MQ=255)                                ggTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTc                                                                                                      >  1:1105744/1‑140 (MQ=255)                                    aTAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCt                                                                                                  <  1:956611/140‑1 (MQ=255)                                     tAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTa                                                                                                 >  1:369383/1‑140 (MQ=255)                                        aTCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGa                                                                                              >  2:396557/1‑140 (MQ=255)                                          ctcCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATa                                                                                            >  1:963523/1‑140 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        >  1:456285/1‑126 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        >  1:75909/1‑126 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  2:75909/126‑1 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  1:545144/126‑1 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  2:456285/126‑1 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        >  2:545144/1‑126 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                          >  1:269442/1‑140 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                          >  2:355251/1‑140 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                          >  1:147587/1‑140 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                          >  1:629171/1‑140 (MQ=255)                                            ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                          >  1:110203/1‑140 (MQ=255)                                             cTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGTGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGc                                                                                         <  2:782854/140‑1 (MQ=255)                                              tGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        <  2:902652/124‑1 (MQ=255)                                              tGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCt                                                                                                        >  1:902652/1‑124 (MQ=255)                                              tGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCg                                                                                        >  1:569053/1‑140 (MQ=255)                                              tGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCg                                                                                        <  2:269442/140‑1 (MQ=255)                                                cAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGTTTAGTAAATGGCGGAAC‑TTTAATTGACTGAAACGCCTCAGCTAGGATAAggggaa                                                                                     >  2:2703/1‑135 (MQ=255)                                                   ggTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACg                                                                                   >  1:650629/1‑140 (MQ=255)                                                    gTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGt                                                                                  <  2:293479/140‑1 (MQ=255)                                                    gTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGt                                                                                  <  1:209908/140‑1 (MQ=255)                                                       ttCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGa                                                                               >  1:676847/1‑140 (MQ=255)                                                        tCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGaa                                                                              <  2:110203/140‑1 (MQ=255)                                                        tCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGaa                                                                              >  1:1205929/1‑140 (MQ=255)                                                          ccTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATa                                                                            <  1:355251/140‑1 (MQ=255)                                                           cTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATaa                                                                           <  2:1105744/140‑1 (MQ=255)                                                              aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                        <  2:1205929/140‑1 (MQ=255)                                                              aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                        <  1:304674/140‑1 (MQ=255)                                                              aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                        >  1:1055752/1‑140 (MQ=255)                                                               gCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAgg                                                                       <  2:569053/140‑1 (MQ=255)                                                               gCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAgg                                                                       <  1:748594/140‑1 (MQ=255)                                                                               gagTAATCCAGCAAATCGTCCGGGGAAACCTTACCCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTcc                                                            >  2:649318/1‑136 (MQ=255)                                                                               gagTAATCCAGCAAATCGTCCGGGGAAACCTTACCCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTcc                                                            <  1:649318/136‑1 (MQ=255)                                                                               gagTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCg                                                       <  1:1098329/140‑1 (MQ=255)                                                                               gagTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCg                                                       >  2:1098329/1‑140 (MQ=255)                                                                                agTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCga                                                      >  2:1179015/1‑140 (MQ=255)                                                                                        aGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACa                                              >  2:318319/1‑140 (MQ=255)                                                                                          cAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                            >  1:1073075/1‑140 (MQ=255)                                                                                          cAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                            >  2:368135/1‑140 (MQ=255)                                                                                          cAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                            >  1:334543/1‑140 (MQ=255)                                                                                          cAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                            >  2:189597/1‑140 (MQ=255)                                                                                          cAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                            >  2:1152680/1‑140 (MQ=255)                                                                                            aaTCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTa                                          >  2:693023/1‑140 (MQ=255)                                                                                              tCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATc                                        <  1:1177020/140‑1 (MQ=255)                                                                                                     gggAAACCTTT‑CCTGTCGGAAAATGCCGATTATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtca                                 <  1:2703/140‑1 (MQ=255)                                                                                                       gAAACCTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtcaca                               <  2:369383/140‑1 (MQ=255)                                                                                                            cTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtcacatcaca                          <  2:438100/140‑1 (MQ=255)                                                                                                            cTTA‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtcacatcaca                          <  2:1144952/140‑1 (MQ=255)                                                                                                               a‑CCTGTCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACtt                       >  1:567865/1‑140 (MQ=255)                                                                                                                     tCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATc                  <  1:318319/140‑1 (MQ=255)                                                                                                                     tCGCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATc                  >  2:821657/1‑140 (MQ=255)                                                                                                                       gCAAACTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATccc                >  1:756640/1‑140 (MQ=255)                                                                                                                            cTGCGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACACACATCACACTTTTATCCCCtttt           >  1:667430/1‑140 (MQ=255)                                                                                                                               cGACTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTtgtg        >  1:469352/1‑140 (MQ=255)                                                                                                                                 aCTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTggg      <  1:1214370/140‑1 (MQ=255)                                                                                                                                 aCTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTggg      <  2:963523/140‑1 (MQ=255)                                                                                                                                  cTGATTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTGGGa     <  2:334543/140‑1 (MQ=255)                                                                                                                                     aTTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTGGGa     <  1:527514/137‑1 (MQ=255)                                                                                                                                     aTTAGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTGGGAAGc  >  2:815024/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTA‑CCTGTCGCAAACTGCGACTGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTGGGAAGC  >  NC_000913/3073655‑3073925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑