Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,288	T→C	S58S (AGT→AGC)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,288	0	T	C	100.0%	272.8 / NA	74	S58S (AGT→AGC)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

TTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGTCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGATATGTCGAAAT  >  NC_000913/3178153‑3178421                                                                                                                                        |                                                                                                                                      ttCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCAt                                                                                                                                   <  2:929685/140‑1 (MQ=255) ttCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCAt                                                                                                                                   <  2:546497/140‑1 (MQ=255)  tCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCAtt                                                                                                                                  >  2:993121/1‑140 (MQ=255)  tCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCAtt                                                                                                                                  >  2:874438/1‑140 (MQ=255)  tCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCAtt                                                                                                                                  >  1:71757/1‑140 (MQ=255)   cTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTa                                                                                                                                 <  2:242174/140‑1 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGc                                                                                                                             >  2:430747/1‑136 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGc                                                                                                                             <  1:430747/136‑1 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  1:637269/1‑140 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  1:535225/1‑140 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  1:50087/1‑140 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  2:448442/1‑140 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  2:859531/1‑140 (MQ=255)           aGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCcaca                                                                                                                         >  2:885371/1‑140 (MQ=255)            gTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAg                                                                                                                        <  2:535225/140‑1 (MQ=255)            gTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAg                                                                                                                        <  2:71757/140‑1 (MQ=255)                gTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTa                                                                                                                                 <  2:1122527/127‑1 (MQ=255)                gTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTa                                                                                                                                 >  1:1122527/1‑127 (MQ=255)                   gAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGtt                                                                                                                 <  2:637269/140‑1 (MQ=255)                     gCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTa                                                                                                               >  1:703453/1‑140 (MQ=255)                         ggCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTg                                                                                                           <  1:874438/140‑1 (MQ=255)                         ggCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTg                                                                                                           <  1:459633/140‑1 (MQ=255)                           ccGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCa                                                                                                         <  1:993121/140‑1 (MQ=255)                               gaATCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGAtt                                                                                                     <  1:885371/140‑1 (MQ=255)                               gaACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGAtt                                                                                                     <  1:634616/140‑1 (MQ=255)                               gaACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGAtt                                                                                                     <  1:448442/140‑1 (MQ=255)                               gaACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGAtt                                                                                                     <  2:531419/140‑1 (MQ=255)                               gaACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGAtt                                                                                                     <  1:963673/140‑1 (MQ=255)                                  ccTCATGCAAGTTTATCAACAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTaca                                                                                                  <  1:1027278/140‑1 (MQ=255)                                     gATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTg                                                                                                           >  2:962355/1‑128 (MQ=255)                                     gATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTg                                                                                                           <  1:962355/128‑1 (MQ=255)                                      aTGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCta                                                                                              <  1:859531/140‑1 (MQ=255)                                      aTGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCta                                                                                              >  2:1049325/1‑140 (MQ=255)                                        gCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCtata                                                                                            <  1:80878/140‑1 (MQ=255)                                                  cAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTa                                                                                  <  1:680779/140‑1 (MQ=255)                                                     caagcaCGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACcg                                                                               >  1:878351/1‑140 (MQ=255)                                                      aagcaCGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACcgc                                                                              >  1:331083/1‑140 (MQ=255)                                                       agcaCGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACcgcg                                                                             >  2:545889/1‑140 (MQ=255)                                                               ttAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACcgcgacgcga                                                                       <  1:1153229/138‑1 (MQ=255)                                                               ttAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACcgcgacgcga                                                                       >  2:1153229/1‑138 (MQ=255)                                                               ttAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAAc                                                                     <  2:331083/140‑1 (MQ=255)                                                                  gTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGc                                                                  >  1:464306/1‑140 (MQ=255)                                                                   tAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCa                                                                 >  2:1117750/1‑140 (MQ=255)                                                                    aaCCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCAt                                                                <  1:834516/140‑1 (MQ=255)                                                                        cGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAAc                                                            >  2:1130441/1‑140 (MQ=255)                                                                        cGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAAc                                                            >  2:583866/1‑140 (MQ=255)                                                                        cGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAAc                                                            >  2:144080/1‑140 (MQ=255)                                                                         ggAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAAct                                                           <  1:1002687/140‑1 (MQ=255)                                                                          gAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAActc                                                          >  2:1111554/1‑140 (MQ=255)                                                                          gAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAActc                                                          >  2:488114/1‑140 (MQ=255)                                                                           aaTTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGaa                                                                      <  2:272659/127‑1 (MQ=255)                                                                           aaTTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGaa                                                                      >  1:272659/1‑127 (MQ=255)                                                                           aaTTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAActct                                                         >  1:1172552/1‑140 (MQ=255)                                                                           aaTTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAActct                                                         >  1:228304/1‑140 (MQ=255)                                                                           aaTTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAActct                                                         >  1:889592/1‑140 (MQ=255)                                                                                  tAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGa                                                  <  2:878351/140‑1 (MQ=255)                                                                                   aaGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAAcgcggc                                                 <  1:1056645/140‑3 (MQ=255)                                                                                   aaGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGAc                                                 <  1:1049325/140‑1 (MQ=255)                                                                                        tGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTg                                            <  2:50087/140‑1 (MQ=255)                                                                                          ccgccgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGAc                                                 <  1:260506/133‑1 (MQ=255)                                                                                          ccgccgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGAc                                                 >  2:260506/1‑133 (MQ=255)                                                                                          ccaccgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCg                                          >  2:917531/4‑140 (MQ=255)                                                                                              cgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCt                                      <  2:889592/140‑1 (MQ=255)                                                                                              cgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCt                                      <  2:1172552/140‑1 (MQ=255)                                                                                              cgATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCCCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCt                                      <  2:228304/140‑1 (MQ=255)                                                                                               gATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCtt                                     <  1:144080/140‑1 (MQ=255)                                                                                               gATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCtt                                     <  1:488114/140‑1 (MQ=255)                                                                                                    tGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGt                                >  2:1051942/1‑140 (MQ=255)                                                                                                            ccTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCaa                        >  2:560626/1‑140 (MQ=255)                                                                                                            ccTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCCAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCaa                        >  2:270939/1‑140 (MQ=255)                                                                                                             cTTTGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAAt                       <  1:917531/140‑1 (MQ=255)                                                                                                               ttGAAAAAATTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATcc                     >  2:125587/1‑140 (MQ=255)                                                                                                                       aTTAATGAAGCGCGCAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGa             <  1:317824/140‑1 (MQ=255)                                                                                                                                  gcgcAGCCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGATATGTCGAAAt  >  2:45711/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCTGGGTTCAGTTCGTTGAGCCAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGTCCATTACTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAACGGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGATATGTCGAAAT  >  NC_000913/3178153‑3178421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑