Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,134	T→C	S340S (AGT→AGC)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,134	0	T	C	100.0%	318.6 / NA	85	S340S (AGT→AGC)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/47);  total (38/47)

AGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACT  >  NC_000913/3178998‑3179267                                                                                                                                         |                                                                                                                                      aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGcc                                                                                                                                    <  1:667520/140‑1 (MQ=255) aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGcc                                                                                                                                    <  1:914152/140‑1 (MQ=255) aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGcc                                                                                                                                    <  1:1017789/140‑1 (MQ=255) aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGcc                                                                                                                                    <  2:122706/140‑1 (MQ=255)    aTGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAgcgc                                                                                                                                     >  2:240531/1‑136 (MQ=255)     tGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATc                                                                                                                                >  2:1219289/1‑140 (MQ=255)     tGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATc                                                                                                                                >  1:338001/1‑140 (MQ=255)      gACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCg                                                                                                                               >  1:26257/1‑140 (MQ=255)           tAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGc                                                                                                                           <  1:471065/139‑1 (MQ=255)           tAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGc                                                                                                                           >  2:471065/1‑139 (MQ=255)             gCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGcgtc                                                                                                                        >  2:1004815/1‑140 (MQ=255)                atatGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTg                                                                                                                     <  2:306397/140‑1 (MQ=255)                   tGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAg                                                                                                                  >  2:656215/1‑140 (MQ=255)                      gCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGAcc                                                                                                               >  2:100397/1‑140 (MQ=255)                      gCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGAcc                                                                                                               >  2:578382/1‑140 (MQ=255)                         aGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTg                                                                                                            <  2:861291/140‑1 (MQ=255)                         aGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTg                                                                                                            <  1:1219289/140‑1 (MQ=255)                         aGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACCGGAAAGCGCCCACCGTAGCGTCGTGCAGACCGTg                                                                                                            <  2:1086079/140‑1 (MQ=255)                           aaCAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCa                                                                                                                   <  1:472554/131‑1 (MQ=255)                           aaCAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCa                                                                                                                   >  2:472554/1‑131 (MQ=255)                             cAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGtt                                                                                                        <  2:158139/140‑1 (MQ=255)                             cAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGtt                                                                                                        <  2:338001/140‑1 (MQ=255)                             cAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGtt                                                                                                        <  1:707958/140‑1 (MQ=255)                               aaGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcc                                                                                                      >  1:408869/1‑140 (MQ=255)                               aaGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcc                                                                                                      <  2:408869/140‑1 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     >  2:72767/1‑140 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     >  2:833633/1‑140 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     <  2:95870/140‑1 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     >  1:796508/1‑140 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     <  1:1032611/140‑1 (MQ=255)                                aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcct                                                                                                     >  1:231192/1‑140 (MQ=255)                                 gTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcctc                                                                                                    <  1:1004815/140‑1 (MQ=255)                                     gCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTc                                                                                                >  2:406133/1‑140 (MQ=255)                                     gCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTc                                                                                                >  2:236227/1‑140 (MQ=255)                                     gCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACACGCAGGTGAAACAGGCACAGTAGAACTGTGTCGGGGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTc                                                                                                >  2:253017/1‑140 (MQ=255)                                       cTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaa                                                                                              <  1:72767/140‑1 (MQ=255)                                       cTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaa                                                                                              <  2:796508/140‑1 (MQ=255)                                       cTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaa                                                                                              <  1:1103675/140‑1 (MQ=255)                                        tGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaac                                                                                             <  1:873161/140‑1 (MQ=255)                                          aGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaacaa                                                                                           >  2:95628/1‑140 (MQ=255)                                             ttCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACAt                                                                                        <  2:309562/140‑1 (MQ=255)                                             ttCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACAt                                                                                        <  2:354706/140‑1 (MQ=255)                                               ctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcc                                                                                                      <  1:440066/124‑1 (MQ=255)                                               ctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTtcc                                                                                                      >  2:440066/1‑124 (MQ=255)                                               ctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTa                                                                                      <  1:946106/140‑1 (MQ=255)                                               ctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTa                                                                                      <  2:505140/140‑1 (MQ=255)                                               ctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTa                                                                                      <  2:888106/140‑1 (MQ=255)                                                tcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTaa                                                                                     <  1:406133/140‑1 (MQ=255)                                                    tGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCa                                                                                 <  1:494034/140‑1 (MQ=255)                                                    tGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCa                                                                                 <  2:26257/140‑1 (MQ=255)                                                              tCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTa                                                                       >  2:187253/1‑140 (MQ=255)                                                                   gCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCAtt                                                                  <  1:95628/140‑1 (MQ=255)                                                                     ggAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTaa                                                                <  1:1076566/140‑1 (MQ=255)                                                                       aaTGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATc                                                                               <  1:600862/123‑1 (MQ=255)                                                                       aaTGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATc                                                                               >  2:600862/1‑123 (MQ=255)                                                                       aaTGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTaa                                                                >  2:1155596/1‑138 (MQ=255)                                                                       aaTGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTaa                                                                <  1:1155596/138‑1 (MQ=255)                                                                            ttAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTac                                                         >  1:265003/1‑140 (MQ=255)                                                                                     aGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGcc                                                >  2:552354/1‑140 (MQ=255)                                                                                                 cacaGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCt                                    <  1:100397/140‑1 (MQ=255)                                                                                                 cacaGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCt                                    >  1:753178/1‑140 (MQ=255)                                                                                                   caGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCa                                  <  1:236227/140‑1 (MQ=255)                                                                                                     gTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCaaa                                >  1:278395/1‑140 (MQ=255)                                                                                                      tACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAg                               <  2:278395/140‑1 (MQ=255)                                                                                                      tACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAg                               <  1:552354/140‑1 (MQ=255)                                                                                                      tACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAg                               >  2:528991/1‑140 (MQ=255)                                                                                                              tGTCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAg                       >  1:911002/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAg                               >  1:640132/1‑130 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAg                               <  2:640132/130‑1 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:291473/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:770499/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:729770/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:1008319/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:127985/1‑140 (MQ=255)                                                                                                                tCGGTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAc                     >  2:179992/1‑140 (MQ=255)                                                                                                                   gTGCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGAcgcg                  <  1:127985/140‑1 (MQ=255)                                                                                                                     gCCAGCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGAt                >  2:1158024/1‑140 (MQ=255)                                                                                                                         gCGAGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGaa            <  1:889861/140‑1 (MQ=255)                                                                                                                            aGCAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCg         <  1:729770/140‑1 (MQ=255)                                                                                                                              cAACTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCggg       >  2:940147/1‑140 (MQ=255)                                                                                                                                 cTGGAAAGCGCCCATCGTAGCGTCGTGCAGACCGTCCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTa    >  1:851320/1‑140 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTCCAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACt  <  1:770499/140‑1 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCg         <  2:315835/133‑1 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCg         >  1:315835/1‑133 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACt  <  1:833633/140‑1 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACt  <  1:1008319/140‑1 (MQ=255)                                                                                                                                   ggAAAGCGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACt  <  1:940147/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATGGAAGCGGGCTACT  >  NC_000913/3178998‑3179267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑