Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,193,489	T→C	L209P (CTG→CCG)	yqiK →	protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,489	0	T	C	100.0%	196.3 / NA	55	L209P (CTG→CCG)	yqiK	protein YqiK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

GTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATG  >  NC_000913/3193353‑3193619                                                                                                                                         |                                                                                                                                   gTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGAc                                                                                                                                 >  2:714719/1‑140 (MQ=255) gTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGAc                                                                                                                                 >  2:551028/1‑140 (MQ=255)   gCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTc                                                                                                                               >  1:1062812/1‑140 (MQ=255)   gCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTc                                                                                                                               >  1:51640/1‑140 (MQ=255)      aaaTACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAgg                                                                                                                            <  1:119823/140‑1 (MQ=255)      aaaTACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAgg                                                                                                                            <  2:356007/140‑1 (MQ=255)                    aagaCCTGTCGAAAAACGATCTGGAACTGGAGATTGTTTCACTTACAAACTTTACCCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACTCCTAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGt                                                                                                              <  2:313373/140‑1 (MQ=255)                        ccTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTcgcc                                                                                                          >  1:801009/1‑140 (MQ=255)                        ccTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTcgcc                                                                                                          <  2:666867/140‑1 (MQ=255)                        ccTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTcgcc                                                                                                          >  2:333990/1‑140 (MQ=255)                          tGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTcgccgc                                                                                                        <  1:714719/140‑1 (MQ=255)                          tGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTcgccgc                                                                                                        >  2:418931/1‑140 (MQ=255)                           gTCGAAAAACGGTCTGGAACTGGCGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCcgcg                                                                                                       >  2:1172009/1‑140 (MQ=255)                                  aaCGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTaa                                                                                                <  2:821086/140‑1 (MQ=255)                                  aaCGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTaa                                                                                                <  1:418931/140‑1 (MQ=255)                                    cGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACg                                                                                              <  1:551028/140‑1 (MQ=255)                                      gTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGaa                                                                                            >  1:754381/1‑140 (MQ=255)                                       tCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAg                                                                                           <  2:270971/140‑1 (MQ=255)                                            aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                                                                                      <  2:1217197/140‑1 (MQ=255)                                            aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                                                                                      <  1:333990/140‑1 (MQ=255)                                            aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                                                                                      <  2:1018635/140‑1 (MQ=255)                                              cTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACa                                                                                    <  2:51640/140‑1 (MQ=255)                                              cTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACa                                                                                    <  2:1122057/140‑1 (MQ=255)                                                 gagaGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCACCGCGAACGTAACGAAGTTGAACAGGa                                                                                 >  2:126909/1‑140 (MQ=255)                                                      cGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAg                                                                            >  1:1176876/1‑140 (MQ=255)                                                         ttCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAg                                                                         <  1:808745/140‑1 (MQ=255)                                                               tACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCgg                                                                   >  1:814240/1‑140 (MQ=255)                                                               tACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCgg                                                                   <  2:1176876/140‑1 (MQ=255)                                                                aCCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGt                                                                  >  1:881667/1‑140 (MQ=255)                                                                          aCCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaaaaa                                                        >  2:617089/1‑140 (MQ=255)                                                                          aCCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaaaaa                                                        >  1:481837/1‑140 (MQ=255)                                                                             aGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAAt                                                     <  2:801009/140‑1 (MQ=255)                                                                               aCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAAt                                                     <  1:767443/138‑1 (MQ=255)                                                                               aCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAAt                                                     >  2:767443/1‑138 (MQ=255)                                                                               aCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATcg                                                   >  2:128271/1‑140 (MQ=255)                                                                               aCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATcg                                                   <  2:754381/140‑1 (MQ=255)                                                                                   cGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAt                                               >  2:785102/1‑140 (MQ=255)                                                                                         aaCATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTa                                         >  1:81435/1‑140 (MQ=255)                                                                                         aaCATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTa                                         >  1:743821/1‑140 (MQ=255)                                                                                           cATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATc                                       >  2:5807/1‑140 (MQ=255)                                                                                              ttCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATcgcg                                    <  1:617089/140‑1 (MQ=255)                                                                                                   tCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGc                               >  1:465744/1‑140 (MQ=255)                                                                                                       aaCAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGga                           >  1:676024/1‑140 (MQ=255)                                                                                                        aCAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGgag                          >  1:753702/1‑140 (MQ=255)                                                                                                         cAATGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGgaga                         <  2:465744/140‑1 (MQ=255)                                                                                                            tGCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTg                      >  1:533915/1‑140 (MQ=255)                                                                                                             gCCTTTGACGCCGACGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGa                     <  2:753702/140‑1 (MQ=255)                                                                                                             gCCTTTGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGa                     <  1:5807/140‑1 (MQ=255)                                                                                                                 ttGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAg                 <  1:128271/140‑1 (MQ=255)                                                                                                                 ttGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAg                 >  2:676686/1‑140 (MQ=255)                                                                                                                 ttGACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAg                 >  1:448727/1‑140 (MQ=255)                                                                                                                   gACGCCGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCa               >  2:446857/1‑140 (MQ=255)                                                                                                                       ccGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCaagaa           <  2:533915/140‑1 (MQ=255)                                                                                                                        cGAAGGTTTAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCaagaag          >  1:1198341/1‑140 (MQ=255)                                                                                                                                tAACCAAACCGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATg  >  2:205915/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   GTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATG  >  NC_000913/3193353‑3193619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑