Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,235,560	G→A	Q106* (CAG→TAG)	rlmG ←	23S rRNA m(2)G1835 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,235,560	0	G	A	100.0%	287.9 / NA	83	Q106* (CAG→TAG)	rlmG	23S rRNA m(2)G1835 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/42);  total (41/42)

GAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTGTTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGCCAGTGCG  >  NC_000913/3235427‑3235696                                                                                                                                      |                                                                                                                                         gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCgg                                                                                                                                    >  1:667540/1‑140 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCgg                                                                                                                                    >  1:330320/1‑140 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCgg                                                                                                                                    >  1:243890/1‑140 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCgg                                                                                                                                    >  2:625121/1‑140 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCgg                                                                                                                                    <  1:129337/140‑1 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCg                                                                                                                                     >  2:1143632/1‑139 (MQ=255) gAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCg                                                                                                                                     <  1:1143632/139‑1 (MQ=255)       tgAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTc                                                                                                                              >  2:314564/1‑140 (MQ=255)              cACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTg                                                                                                                       <  2:243890/140‑1 (MQ=255)               aCGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGc                                                                                                                      <  1:922307/140‑1 (MQ=255)               aCGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGc                                                                                                                      >  1:1155864/1‑140 (MQ=255)                 gggCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTg                                                                                                                    >  2:1063292/1‑140 (MQ=255)                       tGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCgaga                                                                                                              >  2:660441/1‑140 (MQ=255)                               gCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCAc                                                                                                      >  2:334472/1‑140 (MQ=255)                               gCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCAc                                                                                                      >  1:1019357/1‑140 (MQ=255)                                cAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACg                                                                                                     <  2:1008190/140‑1 (MQ=255)                                  ataataCGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCt                                                                                                   <  1:314564/140‑1 (MQ=255)                                     ataCGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGa                                                                                                <  1:296203/140‑1 (MQ=255)                                     ataCGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGa                                                                                                <  1:865725/140‑1 (MQ=255)                                     ataCGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGa                                                                                                <  1:334472/140‑1 (MQ=255)                                        cGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACtc                                                                                             <  1:625121/140‑1 (MQ=255)                                         gtgtATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGa                                                                                                >  1:970708/1‑136 (MQ=255)                                         gtgtATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGa                                                                                                <  2:970708/136‑1 (MQ=255)                                          tgtATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACtcat                                                                                           <  1:111985/140‑1 (MQ=255)                                          tgtATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACtcat                                                                                           >  2:111985/1‑140 (MQ=255)                                             aTCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACg                                                                                                     <  1:809406/127‑1 (MQ=255)                                             aTCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACg                                                                                                     >  2:809406/1‑127 (MQ=255)                                              tCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAAt                                                                                       >  2:1214521/1‑140 (MQ=255)                                                   cGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGt                                                                                  >  2:1048062/1‑140 (MQ=255)                                                   cGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGt                                                                                  >  1:1037919/1‑140 (MQ=255)                                                    gTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCt                                                                                                   >  1:949699/1‑122 (MQ=255)                                                    gTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCt                                                                                                   <  2:949699/122‑1 (MQ=255)                                                     tGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTg                                                                                <  2:884942/140‑1 (MQ=255)                                                       aCCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAg                                                                              >  1:682457/1‑140 (MQ=255)                                                       aCCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAg                                                                              >  1:166842/1‑140 (MQ=255)                                                       aCCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAg                                                                              >  2:1003531/1‑140 (MQ=255)                                                       aCCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAg                                                                              >  2:69168/1‑140 (MQ=255)                                                              tGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTaaa                                                                       >  2:404748/1‑140 (MQ=255)                                                                    gcgcaCGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTcg                                                                 >  1:120061/1‑140 (MQ=255)                                                                     cgcacgcaGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTcgc                                                                >  1:1121110/1‑140 (MQ=255)                                                                       cacgcaGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCAcgcgc                                                              <  2:626828/140‑1 (MQ=255)                                                                        acgcaGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGT‑AATTCTCGCGCGt                                                            >  1:416158/1‑140 (MQ=255)                                                                           caGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCg                                                          >  1:360375/1‑140 (MQ=255)                                                                           caGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCg                                                          <  1:989959/140‑1 (MQ=255)                                                                            aGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGc                                                         <  1:181465/140‑1 (MQ=255)                                                                            aGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGc                                                         <  2:166842/140‑1 (MQ=255)                                                                              ttGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCa                                                       <  2:682457/140‑1 (MQ=255)                                                                               tGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGc                                                         >  2:229174/1‑137 (MQ=255)                                                                               tGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGc                                                         <  1:229174/137‑1 (MQ=255)                                                                                 cTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    <  1:1214521/140‑1 (MQ=255)                                                                                 cTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    <  2:360375/140‑1 (MQ=255)                                                                                 cTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    <  2:1155864/140‑1 (MQ=255)                                                                                 cTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    <  2:120061/140‑1 (MQ=255)                                                                                   gTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCa                                                  <  1:188055/140‑1 (MQ=255)                                                                                    ttCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCAc                                                 <  2:330320/140‑1 (MQ=255)                                                                                        aGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGAt                                             >  2:849784/1‑140 (MQ=255)                                                                                             tGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATg                                        >  2:972293/1‑140 (MQ=255)                                                                                              gCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    <  2:1125519/127‑1 (MQ=255)                                                                                              gCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGtt                                                    >  1:1125519/1‑127 (MQ=255)                                                                                              gCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGa                                       <  1:137913/140‑1 (MQ=255)                                                                                                 aGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCAGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTc                                    >  2:1012942/1‑140 (MQ=255)                                                                                                   tgtTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGc                                  <  1:711035/140‑1 (MQ=255)                                                                                                   tgtTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGc                                  <  2:1121110/140‑1 (MQ=255)                                                                                                   tgtTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGc                                  >  1:651860/1‑140 (MQ=255)                                                                                                    gtTTTCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGcc                                 >  2:1126733/1‑140 (MQ=255)                                                                                                      tttCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCaa                               <  1:1209993/140‑1 (MQ=255)                                                                                                      tttCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCaa                               <  1:1063292/140‑1 (MQ=255)                                                                                                      tttCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCaa                               <  1:972293/140‑1 (MQ=255)                                                                                                      tttCGGCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCAGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCaa                               <  1:1012942/140‑1 (MQ=255)                                                                                                           gCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTg                          <  2:131876/140‑1 (MQ=255)                                                                                                           gCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTg                          <  1:1003531/140‑1 (MQ=255)                                                                                                           gCACTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTg                          <  1:305939/140‑1 (MQ=255)                                                                                                             aCTTTGATCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTa                        >  2:1038189/1‑140 (MQ=255)                                                                                                                    tCAGCACCACACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTa                 <  1:1126733/140‑1 (MQ=255)                                                                                                                        caccacACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGtt             >  2:951322/1‑140 (MQ=255)                                                                                                                         accacACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTc            >  2:198764/1‑140 (MQ=255)                                                                                                                         accacACCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTc            >  2:958458/1‑140 (MQ=255)                                                                                                                             cacCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGt                         >  2:973924/1‑123 (MQ=255)                                                                                                                             cacCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGt                         <  1:973924/123‑1 (MQ=255)                                                                                                                             cacCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGcc        >  2:907841/1‑140 (MQ=255)                                                                                                                             cacCCGGCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGcc        >  1:850884/1‑140 (MQ=255)                                                                                                                                   gCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGCCAGTgcg  <  2:850884/140‑1 (MQ=255)                                                                                                                                   gCTATTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGCCAGTgcg  <  1:1048062/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGATCAGCACCACACCCGGCTGTTGCGGGTAGTCGGCGGTGCTGTCGAGAAACTTCACGCTCGACTCATCAATCCCGTTGAGGCGTAAATTCTCGCGCGTCGCCAGTTCACTGATGTATGAGTCGCCAATGCTGTACGGCTTATGTTCCGCCAGTGCG  >  NC_000913/3235427‑3235696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑