Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,619	T→C	R132R (CGT→CGC)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,619	0	T	C	100.0%	270.3 / NA	79	R132R (CGT→CGC)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/43);  total (36/43)

TGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATC  >  NC_000913/3245483‑3245751                                                                                                                                         |                                                                                                                                     tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCCGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  2:867771/1‑140 (MQ=255) tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  1:908555/1‑140 (MQ=255) tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  1:805927/1‑140 (MQ=255) tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  1:634253/1‑140 (MQ=255) tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  1:40239/1‑140 (MQ=255) tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTc                                                                                                                                    >  2:323769/1‑139 (MQ=255)  gtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  <  2:97667/140‑1 (MQ=255)  gtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  <  2:628730/140‑1 (MQ=255)  gtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  <  2:640569/140‑1 (MQ=255)   ttCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCAt                                                                                                                                 <  2:363692/140‑1 (MQ=255)   ttCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCAt                                                                                                                                 <  2:40239/140‑1 (MQ=255)   ttCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCAt                                                                                                                                 <  2:70762/140‑1 (MQ=255)     cTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCg                                                                                                                               <  1:225048/140‑1 (MQ=255)       gtggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCa                                                                                                                             <  1:548447/140‑1 (MQ=255)        tggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAg                                                                                                                            <  1:267091/140‑1 (MQ=255)        tggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAg                                                                                                                            >  1:97386/1‑140 (MQ=255)        tggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAg                                                                                                                            <  2:840903/140‑1 (MQ=255)        tggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAg                                                                                                                            <  2:779559/140‑1 (MQ=255)          gtgCAACCGCGCTGGCAGGTAGCTGGGTTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTa                                                                                                                          <  2:702493/140‑1 (MQ=255)           tgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGGAAGAGCGCTCCATCGCAGTAg                                                                                                                         >  1:866944/1‑140 (MQ=255)           tgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAg                                                                                                                         >  1:139872/1‑140 (MQ=255)            gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAgg                                                                                                                        <  2:69916/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       <  2:608480/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       >  2:405229/1‑140 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       <  2:446120/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       <  1:885089/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       >  1:612185/1‑140 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       >  2:649923/1‑135 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       <  1:649923/135‑1 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACtt                                                                                                                  <  2:582888/140‑1 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACtt                                                                                                                  >  2:867441/1‑140 (MQ=255)                   cgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTGCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACttt                                                                                                                 >  1:8332/1‑140 (MQ=255)                     cTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTaa                                                                                                               >  1:336590/1‑140 (MQ=255)                         cAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTa                                                                                                           <  2:805927/140‑1 (MQ=255)                              aGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGttc                                                                                                      <  1:960693/140‑1 (MQ=255)                                   gggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTTCCGGCGAAAGAGCGCTCCATCGCAGAAGGCTACTTTAACGTAGGTTCTTCGa                                                                                                 >  1:8503/1‑140 (MQ=255)                                   gggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGa                                                                                                 <  2:612185/140‑1 (MQ=255)                                    ggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAt                                                                                                <  2:866944/140‑1 (MQ=255)                                      tGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTg                                                                                              >  1:292228/1‑140 (MQ=255)                                      tGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGCCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTg                                                                                              <  2:995754/140‑1 (MQ=255)                                       ggCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAAAGTAGGTTCTTCGATTgg                                                                                             >  2:39755/1‑140 (MQ=255)                                         ccTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTg                                                                                           >  2:994429/1‑140 (MQ=255)                                             gCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCgat                                                                                       >  2:444844/1‑140 (MQ=255)                                             gCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGGCTGAAAGCCAGCTCCGAATGGTTCCCGGCGGAAGAGCGCTCCATCGCCGTAGGCTACTTTAAAGTAGGGTCTTCGATTGGTGCgat                                                                                       >  2:305018/1‑140 (MQ=255)                                                    cTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgcg                                                                                <  2:634253/140‑1 (MQ=255)                                                     tCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgcgc                                                                               <  1:994429/140‑1 (MQ=255)                                                       gtggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccg                                                                             >  2:889118/1‑140 (MQ=255)                                                        tggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTTCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                            <  2:131837/140‑1 (MQ=255)                                                        tggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                            <  1:922812/140‑1 (MQ=255)                                                          gtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccg                                                                          <  2:12797/140‑1 (MQ=255)                                                          gtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccg                                                                          <  2:8503/140‑1 (MQ=255)                                                               gtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtggg                                                                     >  1:734882/1‑139 (MQ=255)                                                                tcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtggtg                                                                    <  2:97386/140‑1 (MQ=255)                                                                    tGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTAt                                                                >  2:962727/1‑140 (MQ=255)                                                                       cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                            <  1:77296/125‑1 (MQ=255)                                                                       cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc                                                                            >  2:77296/1‑125 (MQ=255)                                                                       cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATggg                                                             >  1:445914/1‑140 (MQ=255)                                                                       cgcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATggg                                                             <  2:341015/140‑1 (MQ=255)                                                                          ggAAGCAGCGATGGTCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCaa                                                          >  1:605271/1‑140 (MQ=255)                                                                          ggAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCaa                                                          <  2:336590/140‑1 (MQ=255)                                                                                  cGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATg                                                  <  1:962727/140‑1 (MQ=255)                                                                                  cGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATg                                                  <  2:445914/140‑1 (MQ=255)                                                                                    atgatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGca                                                <  2:1107972/140‑1 (MQ=255)                                                                                              cggGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCCTCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAg                                      >  1:262465/1‑140 (MQ=255)                                                                                               gggTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGa                                     <  1:444844/140‑1 (MQ=255)                                                                                                ggTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGAt                                    <  1:405229/140‑1 (MQ=255)                                                                                                 gTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATg                                   >  2:732754/1‑140 (MQ=255)                                                                                                  tCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATgg                                  <  1:889118/140‑1 (MQ=255)                                                                                                   cTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGc                                                 >  2:57528/1‑124 (MQ=255)                                                                                                   cTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGc                                                 <  1:57528/124‑1 (MQ=255)                                                                                                           cAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatca                         >  1:772247/1‑140 (MQ=255)                                                                                                           cAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatca                         >  1:81023/1‑140 (MQ=255)                                                                                                                   aaTGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTcc                    <  2:578637/137‑1 (MQ=255)                                                                                                                   aaTGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTcc                    >  1:578637/1‑137 (MQ=255)                                                                                                                   aaTGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGt                 >  1:200172/1‑140 (MQ=255)                                                                                                                   aaTGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGt                 >  1:523986/1‑140 (MQ=255)                                                                                                                      ggTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCa              <  1:867441/140‑1 (MQ=255)                                                                                                                       gTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             <  2:772247/140‑1 (MQ=255)                                                                                                                                 aaaGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             >  2:81930/1‑130 (MQ=255)                                                                                                                                 aaaGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             <  1:81930/130‑1 (MQ=255)                                                                                                                                  aaGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATc  >  2:591513/1‑140 (MQ=255)                                                                                                                                  aaGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATc  >  2:61673/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATC  >  NC_000913/3245483‑3245751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑