Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,281,723	T→C	intergenic (+98/‑253)	agaA → / → agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,281,723	0	T	C	100.0%	134.7 / NA	37	intergenic (+98/‑253)	agaA/agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

ACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAA  >  NC_000913/3281589‑3281856                                                                                                                                       |                                                                                                                                      acaaATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTAt                                                                                                                                  <  1:1201653/140‑1 (MQ=255) acaaATCTGGATTCAGGGACAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTAt                                                                                                                                  <  1:241435/140‑1 (MQ=255)  caaATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATc                                                                                                                                 <  2:220942/140‑1 (MQ=255)  caaATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATc                                                                                                                                 <  2:235255/140‑1 (MQ=255)  caaATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATc                                                                                                                                 <  1:16875/140‑1 (MQ=255)  caaATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATc                                                                                                                                 <  2:440458/140‑1 (MQ=255)      tCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGAc                                                                                                                             <  2:735561/140‑1 (MQ=255)         ggATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTa                                                                                                                          <  1:536003/140‑1 (MQ=255)          gATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTAc                                                                                                                         <  2:15741/140‑1 (MQ=255)                       ttAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGc                                                                                                            <  1:862247/140‑1 (MQ=255)                            ttCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGc                                                                                                       <  1:547873/140‑1 (MQ=255)                                 tttGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGAt                                                                                                  >  1:1115117/1‑140 (MQ=255)                                                  ttATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCa                                                                                 <  1:601701/140‑1 (MQ=255)                                                  ttATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCa                                                                                 <  2:1115117/140‑1 (MQ=255)                                                          ccTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCaa                                                                         >  1:113930/1‑140 (MQ=255)                                                             tCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATcc                                                                      >  1:979298/1‑140 (MQ=255)                                                              cACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATccc                                                                     >  1:169459/1‑140 (MQ=255)                                                                   cccccGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTg                                                                >  2:837371/1‑140 (MQ=255)                                                                        gTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTcc                                                           >  2:910880/1‑140 (MQ=255)                                                                        gTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTcc                                                           >  1:929557/1‑140 (MQ=255)                                                                         tAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCTGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTGCCt                                                          <  2:1030479/140‑1 (MQ=255)                                                                         tAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCt                                                          >  1:1125855/1‑140 (MQ=255)                                                                                  tctttttctttCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTAtctttt                                                 >  1:196231/1‑140 (MQ=255)                                                                                                          gCAGCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGtt                         <  2:1050976/140‑1 (MQ=255)                                                                                                             gCGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTa                      <  2:929557/140‑1 (MQ=255)                                                                                                              cGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAt                     >  2:662558/1‑140 (MQ=255)                                                                                                              cGGTGTCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAt                     <  1:910880/140‑1 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGTGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                >  1:52317/1‑140 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAAttt                             >  1:630059/1‑127 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAAttt                             <  2:630059/127‑1 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAtt                    <  1:589036/136‑1 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTAtt                    >  2:589036/1‑136 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                >  1:645946/1‑140 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                >  1:1184250/1‑140 (MQ=255)                                                                                                                   tCGGATGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCtt                >  2:1087337/1‑140 (MQ=255)                                                                                                                        tGCGACGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTtc           >  2:231907/1‑140 (MQ=255)                                                                                                                             cGCTAACGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCAt      >  1:1052604/1‑140 (MQ=255)                                                                                                                                 aaCGCGCCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGaa  >  2:497841/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      ACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTTCACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAA  >  NC_000913/3281589‑3281856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑