Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,380	T→C	F191S (TTC→TCC)	diaA →	DnaA initiator‑associating factor for replication initiation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,380	0	T	C	100.0%	233.5 / NA	64	F191S (TTC→TCC)	diaA	DnaA initiator‑associating factor for replication initiation
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/40);  total (24/40)

GGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACT  >  NC_000913/3296247‑3296516                                                                                                                                      |                                                                                                                                         ggcggcGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCa                                                                                                                                    <  2:694556/140‑1 (MQ=255) ggcggcGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCa                                                                                                                                    <  2:399440/140‑1 (MQ=255)  gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCAc                                                                                                                                   <  2:1063058/140‑1 (MQ=255)  gcggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCAc                                                                                                                                   <  2:850432/140‑1 (MQ=255)   cggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCAcc                                                                                                                                  >  1:1011507/1‑140 (MQ=255)    ggcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCa                                                                                                                                 <  2:1169443/140‑1 (MQ=255)     gcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAg                                                                                                                                <  1:1194199/140‑1 (MQ=255)     gcgAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAg                                                                                                                                <  2:1160100/140‑1 (MQ=255)            tGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGAtt                                                                                                                         <  2:1011507/140‑1 (MQ=255)               aGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAg                                                                                                                      <  2:1029066/140‑1 (MQ=255)               aGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAg                                                                                                                      <  1:754985/140‑1 (MQ=255)               aGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAg                                                                                                                      <  2:811768/140‑1 (MQ=255)                 gTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGga                                                                                                                    <  1:171168/140‑1 (MQ=255)                    tGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGaa                                                                                                                 >  1:269257/1‑140 (MQ=255)                        aGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACa                                                                                                             <  2:652430/140‑1 (MQ=255)                           gCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGa                                                                                                          <  2:600830/140‑1 (MQ=255)                           gCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGa                                                                                                          >  2:43468/1‑140 (MQ=255)                             cacaGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAg                                                                                                        <  1:996924/140‑1 (MQ=255)                                     gtgGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATc                                                                                                >  1:967037/1‑140 (MQ=255)                                      tgGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCg                                                                                               >  1:617734/1‑140 (MQ=255)                                            tCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATc                                                                                         >  2:1003210/1‑140 (MQ=255)                                             ccGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg                                                                                        >  1:840721/1‑140 (MQ=255)                                             ccGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg                                                                                        <  1:836406/140‑1 (MQ=255)                                             ccGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg                                                                                        >  1:220545/1‑140 (MQ=255)                                             ccGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCg                                                                                        <  1:43468/140‑1 (MQ=255)                                                cATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAg                                                                                     >  2:64036/1‑140 (MQ=255)                                                  ttCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTc                                                                                   >  2:34198/1‑140 (MQ=255)                                                         gCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTAttt                                                                            <  2:698051/140‑1 (MQ=255)                                                               tAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCa                                                                                      >  2:598266/1‑124 (MQ=255)                                                               tAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCa                                                                                      <  1:598266/124‑1 (MQ=255)                                                                    cTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATTGCCAATCGCAGTCCTTATTTCCGCgctgc                                                                   >  2:345249/1‑138 (MQ=255)                                                                    cTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATTGCCAATCGCAGTCCTTATTTCCGCgctgc                                                                   <  1:345249/138‑1 (MQ=255)                                                                      cGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGtt                                                               <  1:127367/140‑1 (MQ=255)                                                                       gCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTg                                                              >  1:694072/1‑140 (MQ=255)                                                                       gCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTg                                                              <  1:362166/140‑1 (MQ=255)                                                                       gCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTg                                                              <  1:476589/140‑1 (MQ=255)                                                                                 aaTGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTtgtg                                                    >  2:700822/1‑140 (MQ=255)                                                                                     cATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGc                                                >  2:820475/1‑140 (MQ=255)                                                                                     cATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGc                                                <  2:220545/140‑1 (MQ=255)                                                                                     cATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGc                                                <  2:694072/140‑1 (MQ=255)                                                                                       tatGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTg                                                              >  2:548825/1‑124 (MQ=255)                                                                                       tatGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTg                                                              <  1:548825/124‑1 (MQ=255)                                                                                       tatGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCg                                              <  2:617734/140‑1 (MQ=255)                                                                                       tatGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCg                                              <  2:218337/140‑1 (MQ=255)                                                                                          gCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTg                                           <  1:700822/140‑1 (MQ=255)                                                                                             gACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCg                                        <  1:410875/140‑1 (MQ=255)                                                                                              aCGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATCCATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGcc                                         >  2:1112118/1‑138 (MQ=255)                                                                                              aCGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGcc                                         <  1:1112118/138‑1 (MQ=255)                                                                                               cGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTa                                      <  2:840721/140‑1 (MQ=255)                                                                                               cGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTa                                      <  2:1169135/140‑1 (MQ=255)                                                                                                        gCCTGTGCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTAcc                             >  1:235165/1‑140 (MQ=255)                                                                                                           tgtgCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgct                          <  2:922101/140‑1 (MQ=255)                                                                                                            gtgCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTggg                                 >  2:638880/1‑132 (MQ=255)                                                                                                            gtgCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTggg                                 <  1:638880/132‑1 (MQ=255)                                                                                                            gtgCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctg                         >  1:15345/1‑140 (MQ=255)                                                                                                            gtgCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctg                         >  1:10366/1‑140 (MQ=255)                                                                                                              gCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctgct                       <  2:235165/140‑1 (MQ=255)                                                                                                              gCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctgct                       >  2:76408/1‑140 (MQ=255)                                                                                                              gCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctgct                       <  1:579496/140‑1 (MQ=255)                                                                                                              gCGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCgctgct                       <  2:89566/140‑1 (MQ=255)                                                                                                               cGATCTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGctgctg                      <  1:34198/140‑1 (MQ=255)                                                                                                                   cTGATCGATAACACGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTggg                  >  1:245114/1‑140 (MQ=255)                                                                                                                                cGCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCa     >  2:537903/1‑140 (MQ=255)                                                                                                                                 gCTTTCCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCaa    <  2:15345/140‑1 (MQ=255)                                                                                                                                   tttcCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACt  <  2:507401/140‑1 (MQ=255)                                                                                                                                   tttcCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACt  <  2:313174/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACT  >  NC_000913/3296247‑3296516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑