Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,332	T→C	D245G (GAC→GGC)	mlaF ←	intermembrane phospholipid transport system, ATP binding subunit MlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,332	0	T	C	100.0%	253.4 / NA	70	D245G (GAC→GGC)	mlaF	intermembrane phospholipid transport system, ATP binding subunit MlaF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

GGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGTCCAGAAACTGACGTACGCGCGGATCAGGATTCGCCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCAC  >  NC_000913/3339201‑3339468                                                                                                                                    |                                                                                                                                          ggTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAAc                                                                                                                                   >  2:489061/1‑140 (MQ=255)           ccTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTAcgc                                                                                                                         >  2:643467/1‑140 (MQ=255)            cTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTAcgcg                                                                                                                        <  1:534118/140‑1 (MQ=255)            cTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTAcgcg                                                                                                                        <  1:1124134/140‑1 (MQ=255)            cTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTAcgcg                                                                                                                        <  2:855130/140‑1 (MQ=255)               tATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCgg                                                                                                                     >  1:1036137/1‑140 (MQ=255)               tATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCgg                                                                                                                     >  1:461949/1‑140 (MQ=255)                 tGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGAt                                                                                                                   <  2:673032/140‑1 (MQ=255)                 tGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGAt                                                                                                                   >  2:848032/1‑140 (MQ=255)                     cGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAgg                                                                                                               >  2:8626/1‑140 (MQ=255)                       aGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGAt                                                                                                             >  1:436400/1‑140 (MQ=255)                       aGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGAt                                                                                                             >  1:885656/1‑140 (MQ=255)                          gACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTca                                                                                                          <  1:352442/140‑2 (MQ=255)                             gCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCt                                                                                                       >  1:618375/1‑140 (MQ=255)                               cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                     <  2:684200/140‑1 (MQ=255)                               cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                     <  1:465426/140‑1 (MQ=255)                               cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                     <  2:618375/140‑1 (MQ=255)                                  cgcATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAAc                                                                                                  >  2:365455/1‑140 (MQ=255)                                  agcATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGTATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAAc                                                                                                  >  2:873024/2‑140 (MQ=255)                                      tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                     <  2:1033644/133‑1 (MQ=255)                                      tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                     >  1:1033644/1‑133 (MQ=255)                                      tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                              <  1:1151912/140‑1 (MQ=255)                                      tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                              >  2:751784/1‑140 (MQ=255)                                          aCAGCATGAGTGGCTTAACTCACTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGc                                                                                          <  2:221418/140‑1 (MQ=255)                                           cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                         <  1:714977/140‑1 (MQ=255)                                           cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                         <  1:365455/140‑1 (MQ=255)                                           cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                         <  2:885656/140‑1 (MQ=255)                                                 gAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATAAGGATTCACCTGCAACGCCTGGGCACTGCCa                                                                                   >  1:413006/1‑140 (MQ=255)                                                   gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                              >  2:526414/1‑127 (MQ=255)                                                   gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                              <  1:526414/127‑1 (MQ=255)                                                   gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATg                                                                                 >  1:707239/1‑140 (MQ=255)                                                      gCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGc                                                                              <  1:8626/140‑1 (MQ=255)                                                      gCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGc                                                                              >  1:578702/1‑140 (MQ=255)                                                        ttAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGa                                                                            >  2:270566/1‑140 (MQ=255)                                                                 tGGTAAAAGATCAGCGTGATAATCACCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑tttttt                                                                  >  2:677117/1‑140 (MQ=255)                                                                  ggTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑ttttttt                                                                 <  2:61045/140‑1 (MQ=255)                                                                   gTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTg                                                                <  1:751784/140‑1 (MQ=255)                                                                    tAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGt                                                               <  1:560034/140‑1 (MQ=255)                                                                     aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                              <  2:461949/140‑1 (MQ=255)                                                                     aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                              <  2:1036137/140‑1 (MQ=255)                                                                     aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATAAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                              <  2:413006/140‑1 (MQ=255)                                                                          aTCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                         <  1:821905/140‑1 (MQ=255)                                                                             aGCGTGATAATCGCCGGCAGGATAGCGGAACGGAGCAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGa                                                      >  2:1085910/1‑140 (MQ=255)                                                                             aGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGa                                                      >  2:1128669/1‑140 (MQ=255)                                                                              gCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGAt                                                     >  1:1076603/1‑140 (MQ=255)                                                                               cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                         >  1:445696/1‑135 (MQ=255)                                                                               cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                         <  2:445696/135‑1 (MQ=255)                                                                               cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATc                                                    <  1:270566/140‑1 (MQ=255)                                                                               cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATc                                                    >  2:1066783/1‑140 (MQ=255)                                                                                     aaTCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCg                                              <  2:707239/140‑1 (MQ=255)                                                                                       tCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTg                                            <  1:1128669/140‑1 (MQ=255)                                                                                       tCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTg                                            <  1:1066783/140‑1 (MQ=255)                                                                                             gCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGc                                      >  1:761704/1‑140 (MQ=255)                                                                                             gCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGc                                      >  1:906320/1‑140 (MQ=255)                                                                                              cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                     <  1:873024/140‑1 (MQ=255)                                                                                              cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                     <  1:848032/140‑1 (MQ=255)                                                                                              cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                     >  1:1089856/1‑140 (MQ=255)                                                                                                 gATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAgg                                                <  2:514279/126‑1 (MQ=255)                                                                                                 gATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAgg                                                >  1:514279/1‑126 (MQ=255)                                                                                                      cggaacggaaCAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTaa                             <  2:578702/140‑1 (MQ=255)                                                                                                                cAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGc                   >  1:710946/1‑140 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAac                            >  2:349731/1‑124 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAac                            <  1:349731/124‑1 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACATTTCCGGCACATCGTg           >  2:902535/1‑140 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg           >  2:1206114/1‑140 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg           >  1:825794/1‑140 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg           >  1:512270/1‑140 (MQ=255)                                                                                                                        tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg           >  1:1062746/1‑140 (MQ=255)                                                                                                                         cAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTga          <  2:825794/140‑1 (MQ=255)                                                                                                                           gCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTgaga        <  2:1089856/140‑1 (MQ=255)                                                                                                                                 ccGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAaccac  <  1:677117/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                          GGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGTCCAGAAACTGACGTACGCGCGGATCAGGATTCGCCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCAC  >  NC_000913/3339201‑3339468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑