Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,365	G→A	A234V (GCG→GTG)	mlaF ←	intermembrane phospholipid transport system, ATP binding subunit MlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,365	0	G	A	100.0%	216.7 / NA	64	A234V (GCG→GTG)	mlaF	intermembrane phospholipid transport system, ATP binding subunit MlaF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/35);  total (29/35)

GCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGTCCAGAAACTGACGTACGCGCGGATCAGGATTCGCCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTT  >  NC_000913/3339229‑3339495                                                                                                                                         |                                                                                                                                    gCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCt                                                                                                                                  >  1:618375/1‑140 (MQ=255)   cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                                                <  2:684200/140‑1 (MQ=255)   cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                                                <  1:465426/140‑1 (MQ=255)   cAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                                                <  2:618375/140‑1 (MQ=255)      cgcATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAAc                                                                                                                             >  2:365455/1‑140 (MQ=255)      agcATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGTATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAAc                                                                                                                             >  2:873024/2‑140 (MQ=255)          tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                                                >  1:1033644/1‑133 (MQ=255)          tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGc                                                                                                                                <  2:1033644/133‑1 (MQ=255)          tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                                                         >  2:751784/1‑140 (MQ=255)          tttAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                                                         <  1:1151912/140‑1 (MQ=255)              aCAGCATGAGTGGCTTAACTCACTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGc                                                                                                                     <  2:221418/140‑1 (MQ=255)               cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                                                    <  1:365455/140‑1 (MQ=255)               cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                                                    <  2:885656/140‑1 (MQ=255)               cAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCa                                                                                                                    <  1:714977/140‑1 (MQ=255)                     gAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATAAGGATTCACCTGCAACGCCTGGGCACTGCCa                                                                                                              >  1:413006/1‑140 (MQ=255)                       gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                                                         >  2:526414/1‑127 (MQ=255)                       gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCt                                                                                                                         <  1:526414/127‑1 (MQ=255)                       gTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATg                                                                                                            >  1:707239/1‑140 (MQ=255)                          gCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGc                                                                                                         <  1:8626/140‑1 (MQ=255)                          gCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGc                                                                                                         >  1:578702/1‑140 (MQ=255)                            ttAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGa                                                                                                       >  2:270566/1‑140 (MQ=255)                                     tGGTAAAAGATCAGCGTGATAATCACCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑tttttt                                                                                             >  2:677117/1‑140 (MQ=255)                                      ggTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑ttttttt                                                                                            <  2:61045/140‑1 (MQ=255)                                       gTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTg                                                                                           <  1:751784/140‑1 (MQ=255)                                        tAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGt                                                                                          <  1:560034/140‑1 (MQ=255)                                         aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                                                         <  2:461949/140‑1 (MQ=255)                                         aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                                                         <  2:1036137/140‑1 (MQ=255)                                         aaaaGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATAAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTc                                                                                         <  2:413006/140‑1 (MQ=255)                                              aTCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                                                    <  1:821905/140‑1 (MQ=255)                                                 aGCGTGATAATCGCCGGCAGGATAGCGGAACGGAGCAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGa                                                                                 >  2:1085910/1‑140 (MQ=255)                                                 aGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGa                                                                                 >  2:1128669/1‑140 (MQ=255)                                                  gCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGAt                                                                                >  1:1076603/1‑140 (MQ=255)                                                   cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                                                    <  2:445696/135‑1 (MQ=255)                                                   cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCa                                                                                    >  1:445696/1‑135 (MQ=255)                                                   cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATc                                                                               <  1:270566/140‑1 (MQ=255)                                                   cGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATc                                                                               >  2:1066783/1‑140 (MQ=255)                                                         aaTCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCg                                                                         <  2:707239/140‑1 (MQ=255)                                                           tCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTg                                                                       <  1:1128669/140‑1 (MQ=255)                                                           tCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTg                                                                       <  1:1066783/140‑1 (MQ=255)                                                                 gCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGc                                                                 >  1:906320/1‑140 (MQ=255)                                                                 gCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGc                                                                 >  1:761704/1‑140 (MQ=255)                                                                  cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                                                <  1:873024/140‑1 (MQ=255)                                                                  cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                                                >  1:1089856/1‑140 (MQ=255)                                                                  cAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCa                                                                <  1:848032/140‑1 (MQ=255)                                                                     gATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAgg                                                                           >  1:514279/1‑126 (MQ=255)                                                                     gATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAgg                                                                           <  2:514279/126‑1 (MQ=255)                                                                          cggaacggaaCAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTaa                                                        <  2:578702/140‑1 (MQ=255)                                                                                    cAGGCCCGTCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGc                                              >  1:710946/1‑140 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAac                                                       <  1:349731/124‑1 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAATTTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAac                                                       >  2:349731/1‑124 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACATTTCCGGCACATCGTg                                      >  2:902535/1‑140 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg                                      >  1:1062746/1‑140 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg                                      >  2:1206114/1‑140 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg                                      >  1:825794/1‑140 (MQ=255)                                                                                            tCAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTg                                      >  1:512270/1‑140 (MQ=255)                                                                                             cAGCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTga                                     <  2:825794/140‑1 (MQ=255)                                                                                               gCTATCCCGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTgaga                                   <  2:1089856/140‑1 (MQ=255)                                                                                                     ccGCCCAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAaccac                             <  1:677117/140‑1 (MQ=255)                                                                                                        cccAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACcaccac                          >  2:514987/2‑140 (MQ=255)                                                                                                        cccAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACcaccac                          >  2:464524/2‑140 (MQ=255)                                                                                                          cAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACcacac                        <  2:436400/140‑1 (MQ=255)                                                                                                          cAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACcacac                        <  1:902535/140‑1 (MQ=255)                                                                                                          cAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACcacac                        <  1:464524/140‑1 (MQ=255)                                                                                                          cAGAAACTGACGTACGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACcacac                        <  1:1206114/140‑1 (MQ=255)                                                                                                                       aCGCGCGGATCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACCACACAAGTCACGCCCAg           >  2:709635/1‑140 (MQ=255)                                                                                                                                tCAGGATTCACCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGtt  <  1:657148/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGTCCAGAAACTGACGTACGCGCGGATCAGGATTCGCCTGCAACGCCTGGGCACTGCCATGAGCGACAA‑TTTTTTTGTCCGCCAGGATCCAGGCGTGATCCGCAATACTTAACACTTCCGGCACATCGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTT  >  NC_000913/3339229‑3339495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑