Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,029	T→C	V102A (GTT→GCT)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,029	0	T	C	100.0%	179.6 / NA	49	V102A (GTT→GCT)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/30);  total (19/30)

GCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAG  >  NC_000913/3354895‑3355163                                                                                                                                       |                                                                                                                                       gCTGTTACAAAACCCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGACCTCGCCGCTGCCGCACGCCGCATCGCTgaag                                                                                                                                   <  2:582210/140‑1 (MQ=255)             aCCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAAc                                                                                                                       >  1:1028001/1‑140 (MQ=255)             aCCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAAc                                                                                                                       >  1:1211936/1‑140 (MQ=255)                  aTCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGaa                                                                                                                  >  1:366191/1‑140 (MQ=255)                  aTCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGaa                                                                                                                  <  1:198632/140‑1 (MQ=255)                   tCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGaaa                                                                                                                 >  1:1037016/1‑140 (MQ=255)                    cGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAc                                                                                                                <  1:921337/140‑1 (MQ=255)                    cGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTCAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAc                                                                                                                <  1:78392/140‑1 (MQ=255)                     gCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAcc                                                                                                               <  1:1099560/140‑1 (MQ=255)                         ttttCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGt                                                                                                           <  2:51390/140‑1 (MQ=255)                           ttCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGt                                                                                                           <  1:1049511/138‑1 (MQ=255)                           ttCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGt                                                                                                           >  2:1049511/1‑138 (MQ=255)                                 tCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtgtg                                                                                                   >  1:209982/1‑140 (MQ=255)                                   gTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTggg                                                                                                 >  1:637648/1‑140 (MQ=255)                                     tGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCt                                                                                               <  2:458146/140‑1 (MQ=255)                                      gcgcAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTg                                                                                              <  2:1037016/140‑1 (MQ=255)                                           ggAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTg                                                                                         <  1:845496/140‑1 (MQ=255)                                            gAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGc                                                                                            <  1:1219457/136‑1 (MQ=255)                                            gAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGc                                                                                            >  2:1219457/1‑136 (MQ=255)                                             ggcgcGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAt                                                                                       <  2:520264/139‑1 (MQ=255)                                              gcgcGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATg                                                                                      <  2:836964/140‑1 (MQ=255)                                              gcgcGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATg                                                                                      <  2:296729/140‑1 (MQ=255)                                                gcgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTc                                                                                    <  1:173825/140‑1 (MQ=255)                                                 cgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTcc                                                                                   <  2:1211936/140‑1 (MQ=255)                                                   gCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTcccc                                                                                 <  1:527954/140‑1 (MQ=255)                                                    cTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCa                                                                                <  1:143614/140‑1 (MQ=255)                                                      ggCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTg                                                                                         >  2:796358/1‑129 (MQ=255)                                                      ggCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTg                                                                                         <  1:796358/129‑1 (MQ=255)                                                             aGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAg                                                                       <  1:107711/140‑1 (MQ=255)                                                              gCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAgg                                                                      <  2:412886/140‑1 (MQ=255)                                                                                   aTGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcct                                                 <  2:209982/140‑1 (MQ=255)                                                                                        ctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGc                                                    <  1:355058/132‑1 (MQ=255)                                                                                        ctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGc                                                    >  2:355058/1‑132 (MQ=255)                                                                                        ctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcct                                            >  2:1015637/1‑140 (MQ=255)                                                                                        ctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcct                                            >  1:293149/1‑140 (MQ=255)                                                                                        ctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcct                                            <  1:1015637/140‑1 (MQ=255)                                                                                          tCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcctct                                          <  2:293149/140‑1 (MQ=255)                                                                                          tCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcctct                                          <  2:598376/140‑1 (MQ=255)                                                                                           ccTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcctct                                          >  2:31942/1‑139 (MQ=255)                                                                                           ccTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcctct                                          <  1:31942/139‑1 (MQ=255)                                                                                               aaTAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGcc                                     >  1:459666/1‑140 (MQ=255)                                                                                                        ccTGACCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGa                            <  2:1028001/140‑1 (MQ=255)                                                                                                              cTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCctctct                                        <  1:758007/122‑1 (MQ=255)                                                                                                              cTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCctctct                                        >  2:758007/1‑122 (MQ=255)                                                                                                                 gccgcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGTTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAAtttt                   >  2:1141900/1‑140 (MQ=255)                                                                                                                 gccgcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAAtttt                   >  2:429119/1‑140 (MQ=255)                                                                                                                     cTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTtgtg               <  1:429119/140‑1 (MQ=255)                                                                                                                           cACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGcc         >  2:315725/1‑140 (MQ=255)                                                                                                                                  cATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAg  >  1:1186994/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAG  >  NC_000913/3354895‑3355163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑