Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,404,716	G→A	intergenic (+258/‑720)	yhdH → / → accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,716	0	G	A	100.0%	301.0 / NA	86	intergenic (+258/‑720)	yhdH/accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/48);  total (38/48)

TCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGA  >  NC_000913/3404581‑3404852                                                                                                                                        |                                                                                                                                         tcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAcccc                                                                                                                                      >  1:190415/1‑140 (MQ=255) tcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAcccc                                                                                                                                      <  2:360997/140‑1 (MQ=255) tcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAccc                                                                                                                                       <  2:1141048/139‑1 (MQ=255) tcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAccc                                                                                                                                       >  1:1141048/1‑139 (MQ=255)    tcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCctta                                                                                                                                   >  2:454814/1‑140 (MQ=255)         aCTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAcccc                                                                                                                                      <  1:893449/132‑1 (MQ=255)         aCTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAAcccc                                                                                                                                      >  2:893449/1‑132 (MQ=255)            ggAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTc                                                                                                                           <  1:252663/140‑1 (MQ=255)               aaGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTgg                                                                                                                        <  2:224622/140‑1 (MQ=255)                aGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGt                                                                                                                       <  1:1146260/140‑1 (MQ=255)                   cATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTAcc                                                                                                                    <  1:298641/140‑1 (MQ=255)                   cATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTAcc                                                                                                                    <  1:30526/140‑1 (MQ=255)                   cATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTAcc                                                                                                                    <  2:779248/140‑1 (MQ=255)                     tGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTCCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCAt                                                                                                                  <  2:72028/140‑1 (MQ=255)                            gACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGa                                                                                                           >  1:294627/1‑140 (MQ=255)                             aCTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGaa                                                                                                          >  2:88781/1‑140 (MQ=255)                                gACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGaataa                                                                                                       >  1:697549/1‑140 (MQ=255)                                gACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGaataa                                                                                                       <  1:536044/140‑1 (MQ=255)                                 aCCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCGTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGaataat                                                                                                      >  2:1094676/1‑140 (MQ=255)                                           gATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATtttgt                                                                                            >  2:999998/1‑140 (MQ=255)                                             tGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATtttgttt                                                                                          >  1:888491/1‑140 (MQ=255)                                                gcATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGAc                                                                                       <  2:205453/140‑1 (MQ=255)                                                 cATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACt                                                                                      <  2:294627/140‑1 (MQ=255)                                                    tGATACTCCACTAAACAGCTATTATTTATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGAGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGGGAATAATCTGATTTTGTTTGACTACa                                                                                   >  2:756221/1‑140 (MQ=255)                                                     gATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACaa                                                                                  <  2:190415/140‑1 (MQ=255)                                                          tCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTaa                                                                             <  2:888491/140‑1 (MQ=255)                                                            cACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATc                                                                           >  1:16015/1‑140 (MQ=255)                                                            cACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATc                                                                           >  1:789192/1‑140 (MQ=255)                                                            cACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATc                                                                           >  1:888164/1‑140 (MQ=255)                                                              cTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCAc                                                                         >  2:468490/1‑140 (MQ=255)                                                               tAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACt                                                                        <  1:484363/140‑1 (MQ=255)                                                               tAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACt                                                                        <  2:888164/140‑1 (MQ=255)                                                               tAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACt                                                                        <  2:762239/140‑1 (MQ=255)                                                                aaaCAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTc                                                                       >  1:188056/1‑140 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACt                                                                        >  2:766495/1‑135 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACt                                                                        <  1:766495/135‑1 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAAc                                                                   <  1:999998/140‑1 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAAc                                                                   >  1:463025/1‑140 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAAc                                                                   <  2:789192/140‑1 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAAc                                                                   <  2:463025/140‑1 (MQ=255)                                                                    aGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAAc                                                                   >  2:615771/1‑140 (MQ=255)                                                                         ttattGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAtt                                                              >  2:25077/1‑140 (MQ=255)                                                                         ttattGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAtt                                                              <  1:1094676/140‑1 (MQ=255)                                                                         ttattGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAtt                                                              <  1:468490/140‑1 (MQ=255)                                                                         ttattGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCCGATTTTGTTTGACTACAAATTAATCACTCGAACCTAtt                                                              >  1:643422/1‑140 (MQ=255)                                                                           attGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGa                                                                     >  2:1129581/1‑131 (MQ=255)                                                                           attGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGa                                                                     <  1:1129581/131‑1 (MQ=255)                                                                           attGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTa                                                            >  2:894698/1‑140 (MQ=255)                                                                              gATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATg                                                         <  1:307941/140‑1 (MQ=255)                                                                                tACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCt                                                       >  1:383658/1‑140 (MQ=255)                                                                                tACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCt                                                       >  2:568401/1‑140 (MQ=255)                                                                                   gCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAg                                                    <  2:503213/140‑1 (MQ=255)                                                                                   gCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAg                                                    >  2:283888/1‑140 (MQ=255)                                                                                    ccTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGc                                                   >  2:850812/1‑140 (MQ=255)                                                                                     cTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCa                                                  <  2:58295/140‑1 (MQ=255)                                                                                      tCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCAt                                                 >  2:509029/1‑140 (MQ=255)                                                                                        cGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTg                                               >  2:656587/1‑140 (MQ=255)                                                                                        cGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTg                                               >  2:484188/1‑140 (MQ=255)                                                                                         gTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGt                                              >  1:827124/1‑140 (MQ=255)                                                                                            gCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCaa                                           <  1:25077/140‑1 (MQ=255)                                                                                            gCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCaa                                           <  2:485837/140‑1 (MQ=255)                                                                                                 ttAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGt                                      >  2:306272/1‑140 (MQ=255)                                                                                                  tAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGtt                                     <  1:850812/140‑1 (MQ=255)                                                                                                        tATGTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAAtttt                               <  1:712043/140‑1 (MQ=255)                                                                                                           gTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCg                            <  2:16015/140‑1 (MQ=255)                                                                                                           gTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCg                            <  1:306272/140‑1 (MQ=255)                                                                                                           gTTGCTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCg                            <  2:811485/140‑1 (MQ=255)                                                                                                               cTTTGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCCGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCt                        <  2:643422/140‑1 (MQ=255)                                                                                                                tttGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCtt                       >  1:46752/1‑140 (MQ=255)                                                                                                                  tGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTa                     <  1:454814/140‑1 (MQ=255)                                                                                                                  tGCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTa                     >  1:1108334/1‑140 (MQ=255)                                                                                                                   gCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGc                             <  1:1076082/131‑1 (MQ=255)                                                                                                                   gCCTGGGCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGc                             >  2:1076082/1‑131 (MQ=255)                                                                                                                       gggCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCAtt                <  1:484188/140‑1 (MQ=255)                                                                                                                       gggCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCAtt                <  2:46752/140‑1 (MQ=255)                                                                                                                       gggCGACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCAtt                <  1:910284/140‑1 (MQ=255)                                                                                                                           gACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTcaca            <  2:627253/140‑1 (MQ=255)                                                                                                                           gACGCTACGCTTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTcaca            <  1:511945/140‑1 (MQ=255)                                                                                                                            acgctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGGTGAGCATTGTAAATCGGTTAATTTTGCGTGCTTTAGCATTCACAt           <  2:957218/140‑1 (MQ=255)                                                                                                                            acgctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACAt           <  1:88781/140‑1 (MQ=255)                                                                                                                              gctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTa                     <  2:223549/128‑1 (MQ=255)                                                                                                                              gctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTa                     >  1:223549/1‑128 (MQ=255)                                                                                                                              gctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCt         >  2:1057031/1‑140 (MQ=255)                                                                                                                              gctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATCGTCAATCGGTTAATTTTGCGTGCTTTAGCATTcaca            >  1:1145781/1‑137 (MQ=255)                                                                                                                              gctacgctTAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATCGTCAATCGGTTAATTTTGCGTGCTTTAGCATTcaca            <  2:1145781/137‑1 (MQ=255)                                                                                                                                     ttAACCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGa  <  2:383658/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGA  >  NC_000913/3404581‑3404852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑