Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	350,125	T→C	S38S (AGT→AGC)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	350,125	0	T	C	100.0%	288.0 / NA	78	S38S (AGT→AGC)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/43);  total (35/43)

TGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAA  >  NC_000913/349990‑350256                                                                                                                                        |                                                                                                                                    tGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCa                                                                                                                                 >  1:488081/1‑140 (MQ=255)  gATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCaa                                                                                                                                >  2:1065401/1‑140 (MQ=255)      aCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGAc                                                                                                                            <  2:1087310/140‑1 (MQ=255)      aCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGAc                                                                                                                            <  1:279862/140‑1 (MQ=255)       cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCTGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCa                                                                                                                                 <  2:365849/134‑1 (MQ=255)       cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCTGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCa                                                                                                                                 >  1:365849/1‑134 (MQ=255)       cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGAcc                                                                                                                           >  2:645965/1‑140 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGTAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCa                                                                                                                                 <  2:123513/131‑1 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCaa                                                                                                                                <  1:1022544/132‑1 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCaa                                                                                                                                >  2:1022544/1‑132 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCa                                                                                                                                 >  1:123513/1‑131 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGc                                                                                                                        >  2:482759/1‑140 (MQ=255)          tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGc                                                                                                                        >  2:730436/1‑140 (MQ=255)           gACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCa                                                                                                                       >  2:729297/1‑140 (MQ=255)           gACGAAGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCa                                                                                                                       <  2:299390/140‑1 (MQ=255)               aGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTAc                                                                                                                   >  2:286167/1‑140 (MQ=255)               aGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTAc                                                                                                                   <  1:729297/140‑1 (MQ=255)                ggACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTAcc                                                                                                                  <  2:315146/140‑1 (MQ=255)                ggACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTAcc                                                                                                                  <  2:459416/140‑1 (MQ=255)                    aacaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCgg                                                                                                              <  2:113146/140‑1 (MQ=255)                      caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGAc                                                                                                                            >  2:1164927/1‑124 (MQ=255)                      caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGAc                                                                                                                            <  1:1164927/124‑1 (MQ=255)                      caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCt                                                                                                            >  1:420168/1‑140 (MQ=255)                      caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCt                                                                                                            >  2:86819/1‑140 (MQ=255)                       aTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTa                                                                                                           <  1:804404/140‑1 (MQ=255)                        tGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTAc                                                                                                          <  1:983472/140‑1 (MQ=255)                         gAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACg                                                                                                         <  1:645965/140‑1 (MQ=255)                         gAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACg                                                                                                         <  2:420168/140‑1 (MQ=255)                         gAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACg                                                                                                         <  2:760400/140‑1 (MQ=255)                         gAGCGACACAACGATCCTGCAAAACAGTACACATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGAGGGCAATACGGGGCTCTGCGCCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACg                                                                                                         <  2:115100/140‑1 (MQ=255)                          aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGa                                                                                                        <  2:472973/140‑1 (MQ=255)                          aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGa                                                                                                        <  2:249569/140‑1 (MQ=255)                          aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGa                                                                                                        <  2:563953/140‑1 (MQ=255)                           gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGat                                                                                                       >  2:13536/1‑140 (MQ=255)                           gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGat                                                                                                       >  2:435681/1‑140 (MQ=255)                           gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGat                                                                                                       >  1:1191691/1‑140 (MQ=255)                              acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATAtt                                                                                                    >  1:764554/1‑140 (MQ=255)                                 caACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCtt                                                                                                 >  1:521056/1‑140 (MQ=255)                                    cGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGAt                                                                                              <  1:286167/140‑1 (MQ=255)                                    cGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGAt                                                                                              <  2:488081/140‑1 (MQ=255)                                    cGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGAt                                                                                              <  1:363459/140‑1 (MQ=255)                                     gATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATc                                                                                             <  2:521056/140‑1 (MQ=255)                                         cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGc                                                                                         >  2:614952/1‑140 (MQ=255)                                          tGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCg                                                                                        <  1:614952/140‑1 (MQ=255)                                            cAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGaa                                                                                      >  2:511775/1‑140 (MQ=255)                                                  aGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                                                                >  2:538440/1‑140 (MQ=255)                                                    tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                                                              <  2:54169/140‑1 (MQ=255)                                                    tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCg                                                                              >  1:103983/1‑140 (MQ=255)                                                     aCCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGa                                                                             <  2:103983/140‑1 (MQ=255)                                                           gTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTga                                                                       >  1:21035/1‑140 (MQ=255)                                                           gTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTga                                                                       <  1:756886/140‑1 (MQ=255)                                                              aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                                                                    >  2:209972/1‑140 (MQ=255)                                                              aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                                                                    >  1:1200153/1‑140 (MQ=255)                                                                 aaaCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGt                                                                 <  1:730436/140‑1 (MQ=255)                                                                   aCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTgg                                                               <  1:511775/140‑1 (MQ=255)                                                                   aCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTgg                                                               <  1:209972/140‑1 (MQ=255)                                                                     cGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcg                                                             >  2:706155/1‑140 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  1:482759/140‑1 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  2:1191691/140‑1 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  2:764554/140‑1 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  1:435681/140‑1 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  1:1130179/140‑1 (MQ=255)                                                                      gAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  1:662584/140‑1 (MQ=255)                                                                       aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATACTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                                                           <  1:13536/140‑1 (MQ=255)                                                                        aaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            >  2:351036/1‑138 (MQ=255)                                                                        aaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                            <  1:351036/138‑1 (MQ=255)                                                                                        ttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                                          <  2:690810/140‑1 (MQ=255)                                                                                            ggCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACt                                      >  1:784393/1‑140 (MQ=255)                                                                                                ttCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCg                                  >  2:1203248/1‑140 (MQ=255)                                                                                                 tCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGa                                 >  1:801307/1‑140 (MQ=255)                                                                                                   cggcggGCAATACGGCGCTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGAcc                               >  1:935938/1‑140 (MQ=255)                                                                                                                   gcTCTGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgccg               >  2:1163742/1‑140 (MQ=255)                                                                                                                       tGCACCGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTa           <  1:538440/140‑1 (MQ=255)                                                                                                                            cGTGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACaaaa      >  2:1058715/1‑140 (MQ=255)                                                                                                                              tGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACg    >  2:909238/1‑140 (MQ=255)                                                                                                                              tGGGTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACg    >  2:528485/1‑140 (MQ=255)                                                                                                                               gggTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGa   <  2:801307/140‑1 (MQ=255)                                                                                                                                ggTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAACCATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGaa  <  1:391291/140‑1 (MQ=255)                                                                                                                                ggTAAAAGCGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGaa  <  1:528485/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAAACGAA  >  NC_000913/349990‑350256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑