Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,461,111	T→C	V30A (GTT→GCT)	gspG →	Type II secretion system protein GspG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,461,111	0	T	C	100.0%	313.4 / NA	85	V30A (GTT→GCT)	gspG	Type II secretion system protein GspG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/40);  total (45/40)

CTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAA  >  NC_000913/3460975‑3461244                                                                                                                                         |                                                                                                                                      cTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcc                                                                                                                                    <  1:30799/140‑1 (MQ=255)   ccAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTccta                                                                                                                                  <  2:554860/140‑1 (MQ=255)    cAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaa                                                                                                                                 >  2:820678/1‑140 (MQ=255)    cAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaa                                                                                                                                 >  1:54580/1‑140 (MQ=255)      aCCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaacc                                                                                                                               <  2:54580/140‑1 (MQ=255)      aCCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaacc                                                                                                                               >  1:1051179/1‑140 (MQ=255)      aCCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaacc                                                                                                                               >  2:913527/1‑140 (MQ=255)        ctctTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaaccta                                                                                                                             <  1:826741/140‑1 (MQ=255)         tcttctTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTcctaacctaa                                                                                                                            >  2:1218723/1‑140 (MQ=255)           ttcttcAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTTGCTCCTAACCTAATg                                                                                                                          >  1:396725/1‑140 (MQ=255)           ttcttcAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATg                                                                                                                          >  1:1197143/1‑140 (MQ=255)             cttcAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATggg                                                                                                                        <  2:1051179/140‑1 (MQ=255)             cttcAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATggg                                                                                                                        >  2:763773/1‑140 (MQ=255)              ttcAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGc                                                                                                                       >  2:1102327/1‑140 (MQ=255)                 aaCTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAAt                                                                                                                    <  1:1025336/140‑1 (MQ=255)                      aaCTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGa                                                                                                               >  2:458336/1‑140 (MQ=255)                      aaCTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGa                                                                                                               >  2:591137/1‑140 (MQ=255)                       aCTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGaa                                                                                                              >  2:11459/1‑140 (MQ=255)                             tGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCg                                                                                                        >  1:209188/1‑140 (MQ=255)                               attaattaAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGa                                                                                                      >  1:330385/1‑140 (MQ=255)                                ttaattaaGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGAt                                                                                                     <  1:1188729/140‑1 (MQ=255)                                         gAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCaaaaa                                                                                            >  1:255031/1‑140 (MQ=255)                                         gAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCaaaaa                                                                                            >  2:1065352/1‑140 (MQ=255)                                            aGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGcc                                                                                         >  2:1187935/1‑140 (MQ=255)                                            aGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGcc                                                                                         >  2:125734/1‑140 (MQ=255)                                            aGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGcc                                                                                         >  2:471955/1‑140 (MQ=255)                                              cATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGt                                                                                       <  2:255031/140‑1 (MQ=255)                                              cATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGt                                                                                       <  2:330385/140‑1 (MQ=255)                                               atatGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTc                                                                                      <  1:458336/140‑1 (MQ=255)                                                 atGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAg                                                                                    <  1:597772/140‑1 (MQ=255)                                                 atGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAg                                                                                    <  1:471955/140‑1 (MQ=255)                                                  tGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGc                                                                                   >  2:858395/1‑140 (MQ=255)                                                    cgcgcAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGa                                                                                 <  1:591137/140‑1 (MQ=255)                                                    cgcgcAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGa                                                                                 <  2:1197143/140‑1 (MQ=255)                                                    cgcgcAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGa                                                                                 <  1:229685/140‑1 (MQ=255)                                                      cgcAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGata                                                                               >  2:644522/1‑140 (MQ=255)                                                         aaCGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATtg                                                                            >  2:474584/1‑140 (MQ=255)                                                         aaCGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATtg                                                                            <  1:1102327/140‑1 (MQ=255)                                                         aaCGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATtg                                                                            >  2:430683/1‑140 (MQ=255)                                                                       gcgGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAAc                                                              >  2:541240/1‑140 (MQ=255)                                                                       gcgGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAAc                                                              >  1:963681/1‑140 (MQ=255)                                                                        cgGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACg                                                             <  1:1218723/140‑1 (MQ=255)                                                                        cgGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACg                                                             >  1:488054/1‑140 (MQ=255)                                                                           ttttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGccc                                                          <  1:820678/140‑1 (MQ=255)                                                                           ttttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGccc                                                          <  2:209188/140‑1 (MQ=255)                                                                            tttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCt                                                         <  2:39981/140‑1 (MQ=255)                                                                            tttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCt                                                         <  2:727894/140‑1 (MQ=255)                                                                            tttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCt                                                         >  1:85704/1‑140 (MQ=255)                                                                             ttACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCtt                                                        >  1:339694/1‑140 (MQ=255)                                                                              tACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTg                                                       <  1:474584/140‑1 (MQ=255)                                                                               aCATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTGAGCGATATTGTGGCGCTGGAAAACGCCCTTGa                                                      <  2:386482/140‑1 (MQ=255)                                                                                cATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGat                                                     <  2:85704/140‑1 (MQ=255)                                                                                    aCTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGaaaa                                                               >  2:829100/1‑126 (MQ=255)                                                                                    aCTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGaaaa                                                               <  1:829100/126‑1 (MQ=255)                                                                                     cTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTa                                                >  2:939813/1‑140 (MQ=255)                                                                                     cTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTa                                                >  1:669301/1‑140 (MQ=255)                                                                                        gAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACaa                                             >  2:102337/1‑140 (MQ=255)                                                                                        gAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACa                                              <  2:754652/139‑1 (MQ=255)                                                                                        gAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACa                                              >  1:754652/1‑139 (MQ=255)                                                                                             tATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCg                                        <  1:653518/140‑1 (MQ=255)                                                                                             tATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCg                                        <  1:430683/140‑1 (MQ=255)                                                                                             tATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCg                                        <  2:279249/140‑1 (MQ=255)                                                                                              aTGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGa                                       <  1:102337/140‑1 (MQ=255)                                                                                               tggtggTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGa                                       >  2:450572/1‑139 (MQ=255)                                                                                               tggtggTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGa                                       <  1:450572/139‑1 (MQ=255)                                                                                                ggtggtGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACa                                     >  2:665233/1‑140 (MQ=255)                                                                                                  tggtgATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                   <  1:763773/140‑1 (MQ=255)                                                                                                    gtgATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatca                                 >  1:1096025/1‑140 (MQ=255)                                                                                                    gtgATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatca                                 >  1:961279/1‑140 (MQ=255)                                                                                                     tgATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatcat                                <  2:1096025/140‑1 (MQ=255)                                                                                                       aTCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                              <  2:333384/140‑1 (MQ=255)                                                                                                       aTCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                              >  2:991115/1‑140 (MQ=255)                                                                                                       aTCGTCATTATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                              >  2:1103265/1‑140 (MQ=255)                                                                                                               tATCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCaa                      >  2:93688/1‑140 (MQ=255)                                                                                                                 tCGGCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAAcc                    >  2:1047189/1‑140 (MQ=255)                                                                                                                    gCGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACcaca                 <  2:717219/140‑1 (MQ=255)                                                                                                                     cGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAt                          <  1:452043/130‑1 (MQ=255)                                                                                                                     cGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAt                          >  2:452043/1‑130 (MQ=255)                                                                                                                     cGTACTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaa                >  2:298218/1‑140 (MQ=255)                                                                                                                         cTTGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCa            <  1:644522/140‑1 (MQ=255)                                                                                                                           tGCCAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgg          >  1:442258/1‑140 (MQ=255)                                                                                                                              cAGCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGc       <  1:125734/140‑1 (MQ=255)                                                                                                                                gCCTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt     <  1:665233/140‑1 (MQ=255)                                                                                                                                  cTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGa   >  2:161354/1‑140 (MQ=255)                                                                                                                                  cTGGTGGCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGa   <  2:963681/140‑1 (MQ=255)                                                                                                                                   tggtggCTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGaa  <  2:669301/140‑1 (MQ=255)                                                                                                                                    ggtggcTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt     >  2:1170673/1‑136 (MQ=255)                                                                                                                                    ggtggcTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt     <  1:1170673/136‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAA  >  NC_000913/3460975‑3461244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑