Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,721	T→C	E208E (GAA→GAG)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,721	0	T	C	100.0%	269.7 / NA	75	E208E (GAA→GAG)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/32);  total (43/32)

GCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCG  >  NC_000913/3511585‑3511857                                                                                                                                         |                                                                                                                                         gcgGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTcc                                                                                                                                       >  2:643420/1‑140 (MQ=255)               aGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCTTCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGcc                                                                                                                         >  1:498946/1‑140 (MQ=255)               aGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGcc                                                                                                                         <  2:366391/140‑1 (MQ=255)                 cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCaa                                                                                                                       >  1:121394/1‑140 (MQ=255)                   gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCaaaa                                                                                                                     >  2:1066190/1‑140 (MQ=255)                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCaaaaa                                                                                                                    >  2:959133/1‑140 (MQ=255)                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCaaaaa                                                                                                                    >  2:130931/1‑140 (MQ=255)                    cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCaaaaa                                                                                                                    >  2:180917/1‑140 (MQ=255)                          ctctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACAt                                                                                                              <  2:564161/140‑1 (MQ=255)                             tAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCgg                                                                                                           <  1:130931/140‑1 (MQ=255)                               aTCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTc                                                                                                         <  1:643420/140‑1 (MQ=255)                                tCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCa                                                                                                        <  2:138397/140‑1 (MQ=255)                                ccccAGCATGGTCAGTTCGTTTCCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCa                                                                                                        <  1:1213365/139‑1 (MQ=255)                                      cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAttt                                                                                                  >  1:983989/1‑140 (MQ=255)                                      cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGAttt                                                                                                  >  2:190787/1‑140 (MQ=255)                                               ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATccg                                                                                         >  1:357457/1‑140 (MQ=255)                                                tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATccgc                                                                                        >  2:364174/1‑140 (MQ=255)                                                 cGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATccgcc                                                                                       <  2:881612/140‑1 (MQ=255)                                                 cGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATccgcc                                                                                       <  2:420353/140‑1 (MQ=255)                                                  tttttCGGCCACCACGTTCCAGATCACCCCATCGACATCGCCTTTAACAATGCTTTATAACCTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGGCATGATTTTCTGATccgccg                                                                                      <  2:677004/139‑1 (MQ=255)                                                    tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                    <  2:983989/140‑1 (MQ=255)                                                     ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                   >  2:796748/1‑140 (MQ=255)                                                     ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                   <  2:121394/140‑1 (MQ=255)                                                       ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                 <  1:959133/140‑1 (MQ=255)                                                         gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGtt                                                                               >  2:1154316/1‑140 (MQ=255)                                                              cacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                 <  2:37298/133‑1 (MQ=255)                                                              cacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                 >  1:37298/1‑133 (MQ=255)                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                    <  1:113113/140‑1 (MQ=255)                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                    <  1:705193/140‑1 (MQ=255)                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                    <  1:190787/140‑1 (MQ=255)                                                                    ccAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                    <  1:364174/140‑1 (MQ=255)                                                                      aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                  <  1:1066190/140‑1 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                  >  1:72348/1‑133 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                  <  2:72348/133‑1 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  2:305229/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  2:379267/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  2:523962/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  1:1003673/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  2:549597/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  1:757435/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  1:635866/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  1:615578/1‑140 (MQ=255)                                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                           >  1:514541/1‑140 (MQ=255)                                                                                    aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGc                                                    >  1:1080541/1‑140 (MQ=255)                                                                                          ccTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                              >  2:1146449/1‑140 (MQ=255)                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                    >  1:959628/1‑140 (MQ=255)                                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                    >  1:645509/1‑140 (MQ=255)                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                   >  1:973386/1‑140 (MQ=255)                                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                   >  2:915333/1‑140 (MQ=255)                                                                                                       gTTGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa                                 >  1:1169246/1‑140 (MQ=255)                                                                                                         tGTAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAAt                               >  1:704673/1‑140 (MQ=255)                                                                                                           tAAACTCTCGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCa                             >  1:1056716/1‑140 (MQ=255)                                                                                                                  tcGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                      <  2:310666/140‑1 (MQ=255)                                                                                                                  tcGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                      <  2:526731/140‑1 (MQ=255)                                                                                                                  tcGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                      >  2:900352/1‑140 (MQ=255)                                                                                                                  tcGTGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                      >  2:720560/1‑140 (MQ=255)                                                                                                                     tGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                   >  2:38872/1‑140 (MQ=255)                                                                                                                     tGATAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                   <  1:523962/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  1:1146449/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  1:1154316/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  2:1169246/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  1:305229/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  1:720560/140‑1 (MQ=255)                                                                                                                       aTAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                 <  2:757435/140‑1 (MQ=255)                                                                                                                        tAAGAGAGATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc                <  2:172132/140‑1 (MQ=255)                                                                                                                          agagagATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa              >  1:938715/1‑140 (MQ=255)                                                                                                                          agagagATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa              <  2:973386/140‑1 (MQ=255)                                                                                                                          agagagATCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa              >  2:996503/1‑140 (MQ=255)                                                                                                                            agagaTCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg            >  1:554350/1‑140 (MQ=255)                                                                                                                                aTCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg        >  2:80855/1‑140 (MQ=255)                                                                                                                                aTCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg        >  2:553296/1‑140 (MQ=255)                                                                                                                                aTCGACTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg        >  2:433597/1‑140 (MQ=255)                                                                                                                                     cTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc   <  1:38872/140‑1 (MQ=255)                                                                                                                                     cTCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc   <  1:1106018/140‑1 (MQ=255)                                                                                                                                      tCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg  <  1:549597/140‑1 (MQ=255)                                                                                                                                      tCGCTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg  >  2:149063/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCG  >  NC_000913/3511585‑3511857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑