Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,605,975	T→C	R75R (CGT→CGC)	yhhN →	conserved inner membrane enzyme YhhN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,605,975	0	T	C	100.0%	340.9 / NA	92	R75R (CGT→CGC)	yhhN	conserved inner membrane enzyme YhhN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/41);  total (51/41)

TAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGA  >  NC_000913/3605848‑3606108                                                                                                                                |                                                                                                                                      tAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGcc                                                                                                                           >  2:310360/1‑140 (MQ=255)       ttcttcTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGgc                                                                                                                     <  1:1145595/140‑1 (MQ=255)               ctgctgTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGttttt                                                                                                             >  1:683110/1‑140 (MQ=255)                 gctgTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTcc                                                                                                           >  1:651539/1‑140 (MQ=255)                 gctgTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTcc                                                                                                           >  2:706184/1‑140 (MQ=255)                  ctgTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCct                                                                                                          >  2:182681/1‑140 (MQ=255)                       aCTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                     >  2:1068676/1‑140 (MQ=255)                       aCTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                     >  2:395054/1‑140 (MQ=255)                       aCTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGc                                                                                                     <  2:683110/140‑1 (MQ=255)                         tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  1:182681/140‑1 (MQ=255)                         tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  1:1146046/140‑1 (MQ=255)                         tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  2:13014/140‑1 (MQ=255)                         tggcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  1:310360/140‑1 (MQ=255)                           gcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACct                                                                                                 >  1:1208200/1‑140 (MQ=255)                           gcctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACct                                                                                                 >  1:1140856/1‑140 (MQ=255)                             ctggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACctgc                                                                                               <  2:651539/140‑1 (MQ=255)                               ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  2:726409/134‑1 (MQ=255)                               ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   >  1:726409/1‑134 (MQ=255)                               ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                             >  2:38187/1‑140 (MQ=255)                               ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                             >  1:699287/1‑140 (MQ=255)                               ggcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACctgctg                                                                                             >  2:1098820/1‑140 (MQ=255)                                gcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGt                                                                                            <  1:38187/140‑1 (MQ=255)                                gcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGt                                                                                            <  1:592162/140‑1 (MQ=255)                                gcAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGt                                                                                            >  2:186231/1‑140 (MQ=255)                                  aaGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTac                                                                                          <  2:794297/140‑1 (MQ=255)                                     cgcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCctct                                                                                                        >  1:869136/1‑123 (MQ=255)                                     cgcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCctct                                                                                                        <  2:869136/123‑1 (MQ=255)                                     cgcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACAcc                                                                                       >  1:322476/1‑140 (MQ=255)                                     cgcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACAcc                                                                                       >  1:505368/1‑140 (MQ=255)                                      gcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCa                                                                                      <  1:395054/140‑1 (MQ=255)                                      gcCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCa                                                                                      <  1:186231/140‑1 (MQ=255)                                          aTGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   <  1:286760/123‑1 (MQ=255)                                          aTGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCAc                                                                                                   >  2:286760/1‑123 (MQ=255)                                          aTGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTa                                                                                  >  2:604541/1‑140 (MQ=255)                                          aTGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTa                                                                                  >  1:254400/1‑140 (MQ=255)                                              tCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTc                                                                              >  2:152154/1‑140 (MQ=255)                                                gACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGc                                                                            <  2:322476/140‑1 (MQ=255)                                                          gCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATg                                                                  >  1:559486/1‑140 (MQ=255)                                                              tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              <  2:559486/140‑1 (MQ=255)                                                              tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              <  2:254400/140‑1 (MQ=255)                                                              tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              >  2:484088/1‑140 (MQ=255)                                                              tCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              <  2:1140856/140‑1 (MQ=255)                                                               cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGct                                                             >  2:1062814/1‑140 (MQ=255)                                                               cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              <  1:357264/139‑1 (MQ=255)                                                               cTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              >  2:357264/1‑139 (MQ=255)                                                                 ggTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctct                                                           >  1:543180/1‑140 (MQ=255)                                                                  gTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctctc                                                          >  2:711545/1‑140 (MQ=255)                                                                   tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctctct                                                         >  2:684665/1‑140 (MQ=255)                                                                   tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctctct                                                         >  2:560631/1‑140 (MQ=255)                                                                   tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              <  2:884895/135‑1 (MQ=255)                                                                   tGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGc                                                              >  1:884895/1‑135 (MQ=255)                                                                    gCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCtctt                                                        >  2:63625/1‑140 (MQ=255)                                                                        gCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTTCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctct                                                           <  2:158783/133‑1 (MQ=255)                                                                        gCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTTCGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGctct                                                           >  1:158783/1‑133 (MQ=255)                                                                        gCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCtctttctt                                                    >  2:1210262/1‑140 (MQ=255)                                                                         cAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTttcttc                                                   <  2:1208200/140‑1 (MQ=255)                                                                           gggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTg                                                 <  2:699287/140‑1 (MQ=255)                                                                           gggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTg                                                 <  1:1062814/140‑1 (MQ=255)                                                                           gggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTg                                                 <  1:711545/140‑1 (MQ=255)                                                                            ggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTgg                                                >  1:239048/1‑140 (MQ=255)                                                                            ggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTgg                                                >  2:631283/1‑140 (MQ=255)                                                                            ggCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTgg                                                <  2:1179962/140‑1 (MQ=255)                                                                             gCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGc                                               >  1:160067/1‑140 (MQ=255)                                                                               tgtgCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTg                                                 <  2:175606/136‑1 (MQ=255)                                                                               tgtgCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTg                                                 >  1:175606/1‑136 (MQ=255)                                                                                gtgCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCctc                                            >  2:691086/1‑140 (MQ=255)                                                                                 tgCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCctct                                           <  1:218053/140‑1 (MQ=255)                                                                                  gcgcCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTa                                          >  2:275586/1‑140 (MQ=255)                                                                                  gcgcCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTa                                          >  1:852614/1‑140 (MQ=255)                                                                                      cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCGCGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCCCTCTTTCTTCTGGCCTCTACCAc                                      >  2:1021277/1‑140 (MQ=255)                                                                                      cTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCAc                                      <  1:200845/140‑1 (MQ=255)                                                                                           tgctgGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGtg                                 <  2:505368/140‑1 (MQ=255)                                                                                              tgGGCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGtgctg                              <  2:160067/140‑1 (MQ=255)                                                                                                ggCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTgctgct                            >  2:332268/1‑140 (MQ=255)                                                                                                ggCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTgctgct                            >  1:134594/1‑140 (MQ=255)                                                                                                ggCGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTgctgct                            >  1:95407/1‑140 (MQ=255)                                                                                                  cGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTgg                          >  1:679604/1‑140 (MQ=255)                                                                                                  cGATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTgg                          >  1:1192045/1‑140 (MQ=255)                                                                                                   gATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGtgctgc                             >  1:103326/1‑136 (MQ=255)                                                                                                   gATGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGtgctgc                             <  2:103326/136‑1 (MQ=255)                                                                                                     tGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTc                       <  2:852614/140‑1 (MQ=255)                                                                                                     tGCGCTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTc                       >  1:504623/1‑140 (MQ=255)                                                                                                      gcgcTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCt                      <  2:1192045/140‑1 (MQ=255)                                                                                                         cTAACCCTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTggg                   <  2:112671/140‑1 (MQ=255)                                                                                                             cccTGTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTgcg               <  2:134594/140‑1 (MQ=255)                                                                                                                 gTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGt           >  2:214747/1‑140 (MQ=255)                                                                                                                 gTTGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGt           <  1:684665/140‑1 (MQ=255)                                                                                                                  ttGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTTCGCTGtc          >  2:779614/1‑139 (MQ=255)                                                                                                                  ttGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGtt          >  2:376826/1‑140 (MQ=255)                                                                                                                  ttGCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGtt          >  2:271648/1‑140 (MQ=255)                                                                                                                    gCCACGTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTAc        <  2:504623/140‑1 (MQ=255)                                                                                                                         gTCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCg   <  2:95407/140‑1 (MQ=255)                                                                                                                          tCAACGCCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGa  <  1:63625/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      TAACCCTTCTTCTCCTGCTGTTACTGGCCTGGCAAGCGCCGATGTTCGACGCCATTAGCTATCTGGTGCTGGCAGGGCTGTGCGCCTCACTGCTGGGCGATGCGCTAACCCTGTTGCCACGTCAACGTCTGATGTACGCCATCGGCGCGTTTTTCCTCTCGCACCTGCTGTACACCATCTATTTCGCCAGTCAGATGACGCTCTCTTTCTTCTGGCCTCTACCACTGGTGCTGCTGGTTCTGGGTGCGCTGTTACTGGCGA  >  NC_000913/3605848‑3606108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑