Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,639,218	G→A	intergenic (+77/+167)	pitA → / ← uspB	metal phosphate:H(+) symporter PitA/putative universal stress (ethanol tolerance) protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,639,218	0	G	A	100.0%	177.0 / NA	51	intergenic (+77/+167)	pitA/uspB	metal phosphate:H(+) symporter PitA/putative universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/29);  total (22/29)

TCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCG  >  NC_000913/3639084‑3639353                                                                                                                                       |                                                                                                                                        tCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTg                                                                                                                                    >  1:1099840/1‑140 (MQ=255) tCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTg                                                                                                                                    >  2:453797/1‑140 (MQ=255) tCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTg                                                                                                                                    <  2:777965/140‑1 (MQ=255) tCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTg                                                                                                                                    <  1:673366/140‑1 (MQ=255)   cGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGaa                                                                                                                                  <  1:130602/140‑1 (MQ=255)            tACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATc                                                                                                                         <  2:435442/140‑1 (MQ=255)                gCTTTCCGGCGGGCTGTACTGGCTATCTTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGATCGCCTTATCCGGc                                                                                                                     <  2:421746/140‑1 (MQ=255)                   ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTa                                                                                                                  >  2:83547/1‑140 (MQ=255)                   ttCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTa                                                                                                                  >  2:854056/1‑140 (MQ=255)                    tCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCg                                                                                                                       <  2:1004184/134‑1 (MQ=255)                    tCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCg                                                                                                                       >  1:1004184/1‑134 (MQ=255)                      cggcggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACaa                                                                                                               >  1:216663/1‑140 (MQ=255)                         cggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACaaaaa                                                                                                            >  1:24624/1‑140 (MQ=255)                         cggGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACaaaaa                                                                                                            >  1:296754/1‑140 (MQ=255)                              tGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGc                                                                                                       <  1:115229/140‑1 (MQ=255)                              tGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGc                                                                                                       <  1:15537/140‑1 (MQ=255)                              tGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGc                                                                                                       <  1:83547/140‑1 (MQ=255)                              tGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGc                                                                                                       <  1:208657/140‑1 (MQ=255)                                       tctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAAt                                                                                              <  1:436994/140‑1 (MQ=255)                                        ctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                                                                      >  1:253799/1‑131 (MQ=255)                                        ctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCa                                                                                                      <  2:253799/131‑1 (MQ=255)                                        ctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATa                                                                                             <  1:854056/140‑1 (MQ=255)                                        ctcCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATa                                                                                             >  1:139473/1‑140 (MQ=255)                                          ccTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGa                                                                                           >  1:326094/1‑140 (MQ=255)                                              gCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGc                                                                                       <  2:703194/140‑1 (MQ=255)                                               cAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                                                      <  2:1111233/140‑1 (MQ=255)                                               cAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                                                      <  2:902104/140‑1 (MQ=255)                                               cAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                                                      <  2:24624/140‑1 (MQ=255)                                                   tCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                                                      <  1:1190428/136‑1 (MQ=255)                                                   tCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCa                                                                                      >  2:1190428/1‑136 (MQ=255)                                                   tCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAgaga                                                                                  <  2:395569/140‑1 (MQ=255)                                                   tCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAgaga                                                                                  <  2:139473/140‑1 (MQ=255)                                                    ccTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAt                                                                                 >  1:307381/1‑140 (MQ=255)                                                     cTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGc                                                                                       >  1:47686/1‑133 (MQ=255)                                                     cTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGc                                                                                       <  2:47686/133‑1 (MQ=255)                                                     cTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAtt                                                                                <  1:453797/140‑1 (MQ=255)                                                        tAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATg                                                                             <  2:1099840/140‑1 (MQ=255)                                                        tAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATg                                                                             <  2:307381/140‑1 (MQ=255)                                                             gTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGc                                                                        <  1:913213/140‑1 (MQ=255)                                                              tACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAgaga                                                                                  >  1:439990/1‑129 (MQ=255)                                                              tACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAgaga                                                                                  <  2:439990/129‑1 (MQ=255)                                                              tACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGcc                                                                       >  1:943379/1‑140 (MQ=255)                                                                cGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTg                                                                     >  2:936486/1‑140 (MQ=255)                                                                  cACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGAt                                                                   <  2:142235/140‑1 (MQ=255)                                                                                       tGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                              >  2:28624/1‑140 (MQ=255)                                                                                           ccGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTg                                          >  2:439126/1‑140 (MQ=255)                                                                                                                 gcgcTTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCg                    <  1:333706/140‑1 (MQ=255)                                                                                                                     tttGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTc                <  2:216663/140‑1 (MQ=255)                                                                                                                      ttGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCa               >  1:445404/1‑140 (MQ=255)                                                                                                                         ttCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGtt            >  1:82255/1‑140 (MQ=255)                                                                                                                                   aTGCAGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCg  >  2:913715/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGATTGTGACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCG  >  NC_000913/3639084‑3639353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑