Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,743,852	T→C	V37A (GTT→GCT)	yiaL →	DUF386 domain‑containing protein YiaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,852	0	T	C	100.0%	204.9 / NA	56	V37A (GTT→GCT)	yiaL	DUF386 domain‑containing protein YiaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/25);  total (31/25)

TATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCGG  >  NC_000913/3743727‑3743987                                                                                                                              |                                                                                                                                        tATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACg                                                                                                                           >  1:1195156/1‑140 (MQ=255)        agaTAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCaaaaa                                                                                                                    >  2:210872/1‑140 (MQ=255)         gaTAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAat                                                                                                                   >  2:615189/1‑140 (MQ=255)          aTAAGTCATGAATTTTGTACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAata                                                                                                                  >  2:929870/1‑140 (MQ=255)                cATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTtata                                                                                                            >  1:747474/1‑140 (MQ=255)                 aTGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATAc                                                                                                           >  2:397144/1‑140 (MQ=255)                  tGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACg                                                                                                          <  1:929756/140‑1 (MQ=255)                    aTTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCa                                                                                                        >  1:610643/1‑140 (MQ=255)                    aTTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCa                                                                                                        <  2:501266/140‑1 (MQ=255)                        ttGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAAtt                                                                                                    >  2:715800/1‑140 (MQ=255)                          ggACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTAt                                                                                                  <  2:747474/140‑1 (MQ=255)                                  cgcgCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTaa                                                                                          >  2:22315/1‑140 (MQ=255)                                   gcgcAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                         <  1:22315/140‑1 (MQ=255)                                   gcgcAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                         <  2:610643/140‑1 (MQ=255)                                         cccAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACAcg                                                                                   <  2:57312/140‑1 (MQ=255)                                         cccAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACAcg                                                                                   >  1:416172/1‑140 (MQ=255)                                              tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  2:275052/140‑1 (MQ=255)                                              tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:929870/140‑1 (MQ=255)                                              tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:615189/140‑1 (MQ=255)                                              tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:210872/140‑1 (MQ=255)                                              tCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:397144/140‑1 (MQ=255)                                                  tGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGACAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:562145/136‑1 (MQ=255)                                                  tGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGACAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              >  2:562145/1‑136 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                         <  1:650735/124‑1 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAAc                                                                                         >  2:650735/1‑124 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  2:965549/135‑1 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              <  1:222749/135‑1 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              >  1:965549/1‑135 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAg                                                                              >  2:222749/1‑135 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                         >  1:648572/1‑140 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                         >  2:942704/1‑140 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                         >  1:264728/1‑140 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                         >  1:336264/1‑140 (MQ=255)                                                   gCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCggtg                                                                         >  2:1204368/1‑140 (MQ=255)                                                       tttGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTaa                                                                     >  1:406852/1‑140 (MQ=255)                                                        ttGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTaaa                                                                    <  1:21589/140‑1 (MQ=255)                                                        ttGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTaaa                                                                    <  1:917803/140‑1 (MQ=255)                                                                cgccATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCgg                                                            >  2:933400/1‑140 (MQ=255)                                                                    aTTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGt                                                        <  1:765427/140‑1 (MQ=255)                                                                        aaaaGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAt                                                    <  2:1093551/140‑1 (MQ=255)                                                                           aGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgt                                                 <  1:942704/140‑1 (MQ=255)                                                                           aGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCAtcgt                                                 <  1:785548/140‑1 (MQ=255)                                                                                cTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGta                                            >  1:386977/1‑140 (MQ=255)                                                                                           gcgcgcTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTc                                 <  1:715800/140‑1 (MQ=255)                                                                                             gcgcTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCg                                       <  2:452419/132‑1 (MQ=255)                                                                                             gcgcTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCg                                       >  1:452419/1‑132 (MQ=255)                                                                                              cgcTACCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGt                              >  1:1060289/1‑140 (MQ=255)                                                                                                  aCCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTatat                                         >  1:737320/1‑125 (MQ=255)                                                                                                  aCCGATTTCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTatat                                         <  2:737320/125‑1 (MQ=255)                                                                                                       tttCAACGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGt                     <  1:1204368/140‑1 (MQ=255)                                                                                                           aaCGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTgggg                 >  2:280695/1‑140 (MQ=255)                                                                                                           aaCGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTgggg                 >  2:960575/1‑140 (MQ=255)                                                                                                           aaCGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTgggg                 >  1:354661/1‑140 (MQ=255)                                                                                                             cGCCCTGGAGCCGGGCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCg               >  2:822853/1‑140 (MQ=255)                                                                                                                          ggCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCgg  >  2:889944/1‑140 (MQ=255)                                                                                                                          ggCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCgg  >  1:21311/1‑140 (MQ=255)                                                                                                                          ggCGCTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCgg  >  1:96959/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                        TATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCGCTCGATTTTCTGCGCGCTACCGATTTCAACGCCCTGGAGCCGGGCGTTGTCGAAATCGACGGCAAAAATATTTATACGCAAATTATCGATTTAACCACACGCGAAGCGGTGGTAAACCGTCCGGAAGTGCATCGTCGGTATATCGATATTCAGTTTCTGGCGTGGGGCGAAGAGAAAATCGG  >  NC_000913/3743727‑3743987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑