Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,756,719	G→A	A370V (GCA→GTA)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,756,719	0	G	A	100.0%	183.8 / NA	53	A370V (GCA→GTA)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/30);  total (23/30)

CTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGT  >  NC_000913/3756584‑3756854                                                                                                                                        |                                                                                                                                        cTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTg                                                                                                                                     >  1:120784/1‑140 (MQ=255)   ttACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGa                                                                                                                                   >  2:1167226/1‑140 (MQ=255)   ttACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTACCAGCCGTTGTCGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGa                                                                                                                                   <  1:1042237/140‑1 (MQ=255)    tACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGAt                                                                                                                                  <  1:344118/140‑1 (MQ=255)    tACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGAt                                                                                                                                  <  1:829945/140‑1 (MQ=255)    tACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGAt                                                                                                                                  <  1:178721/140‑1 (MQ=255)    tACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGAt                                                                                                                                  <  2:927018/140‑1 (MQ=255)     aCGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATc                                                                                                                                 >  1:57844/1‑140 (MQ=255)     aCGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATc                                                                                                                                 >  1:1129244/1‑140 (MQ=255)         cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGt                                                                                                                             >  2:1162694/1‑140 (MQ=255)              aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGGGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTa                                                                                                                        <  2:625355/140‑1 (MQ=255)               tCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTaa                                                                                                                       >  1:581131/1‑140 (MQ=255)                     aTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCcac                                                                                                                 <  2:1027271/140‑1 (MQ=255)                     aTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCcac                                                                                                                 <  1:1167226/140‑1 (MQ=255)                      tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCcaca                                                                                                                >  2:523626/1‑140 (MQ=255)                      tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCcac                                                                                                                 >  2:536272/1‑139 (MQ=255)                      tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCcac                                                                                                                 <  1:536272/139‑1 (MQ=255)                        tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAgg                                                                                                              <  1:313111/140‑1 (MQ=255)                         aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGc                                                                                                             <  2:581131/140‑1 (MQ=255)                                aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGTGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTc                                                                                                      <  1:1099635/140‑1 (MQ=255)                                aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTc                                                                                                      <  1:172354/140‑1 (MQ=255)                                aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTc                                                                                                      <  2:1081301/140‑1 (MQ=255)                                       cAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCAt                                                                                               <  1:704791/140‑1 (MQ=255)                                         gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTa                                                                                             <  2:57844/140‑1 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTa                                                                                             >  1:822634/1‑137 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTa                                                                                             <  2:822634/137‑1 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTc                                                                                          >  2:456508/1‑140 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTc                                                                                          >  2:406962/1‑140 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTc                                                                                          >  2:325176/1‑140 (MQ=255)                                            aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTc                                                                                          >  2:2590/1‑140 (MQ=255)                                                ttGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCa                                                                                      >  2:185268/1‑140 (MQ=255)                                                     aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGTATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATAcc                                                                                 <  2:1110154/140‑1 (MQ=255)                                                        gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACcgcg                                                                              <  2:120784/140‑1 (MQ=255)                                                                     tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATcttcttctt                                                                 >  1:945565/1‑140 (MQ=255)                                                                                gtgGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAgg                                                      <  1:1162694/140‑1 (MQ=255)                                                                                gtgGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAgg                                                      <  2:463584/140‑1 (MQ=255)                                                                                           gatTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCGATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGcc                                           <  1:325176/140‑1 (MQ=255)                                                                                            atTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCt                                          >  1:1186329/1‑140 (MQ=255)                                                                                              tACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGc                                        >  1:718666/1‑140 (MQ=255)                                                                                                 aTCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCgg                                     <  1:2590/140‑1 (MQ=255)                                                                                                  tCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCggg                                    >  1:944094/1‑140 (MQ=255)                                                                                                  tCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCggg                                    <  1:185268/140‑1 (MQ=255)                                                                                                     cTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGata                                 >  1:16397/1‑140 (MQ=255)                                                                                                      tGCGCGATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGatat                                <  2:718666/140‑1 (MQ=255)                                                                                                        cgcgATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATcc                              >  1:669776/1‑140 (MQ=255)                                                                                                        cgcgATAAATCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATcc                              >  1:434763/1‑140 (MQ=255)                                                                                                                aTCGCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTttctt                      <  1:456508/140‑1 (MQ=255)                                                                                                                   gCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGc                   <  1:323086/140‑1 (MQ=255)                                                                                                                   gCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGc                   <  2:669776/140‑1 (MQ=255)                                                                                                                   gCCACAATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGc                   <  2:945565/140‑1 (MQ=255)                                                                                                                     cacaATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCa                 >  2:151012/1‑140 (MQ=255)                                                                                                                       caATTTCTTCGTGAGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGt               >  2:786698/1‑140 (MQ=255)                                                                                                                                    aGTTACCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGt  <  2:434763/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGT  >  NC_000913/3756584‑3756854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑