Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,514	C→T	intergenic (‑4/+73)	lacI ← / ← mhpR	DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,514	0	C	T	100.0%	182.2 / NA	52	intergenic (‑4/+73)	lacI/mhpR	DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

CTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCC  >  NC_000913/367379‑367646                                                                                                                                         |                                                                                                                                     cTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTtacca                                                                                                                                  >  1:663238/1‑140 (MQ=255)   ccGACATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTCGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTaccacc                                                                                                                                <  2:792862/140‑1 (MQ=255)    cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCAccc                                                                                                                               >  2:449073/1‑140 (MQ=255)    cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCAccc                                                                                                                               >  2:870996/1‑140 (MQ=255)    cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCAccc                                                                                                                               >  1:1201417/1‑140 (MQ=255)      ccATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTaccacc                                                                                                                                <  1:1067743/137‑1 (MQ=255)      ccATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTaccacc                                                                                                                                >  2:1067743/1‑137 (MQ=255)      ccATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTg                                                                                                                             >  1:48981/1‑140 (MQ=255)                ttCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGActct                                                                                                                   >  1:509182/1‑140 (MQ=255)                   cACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCtt                                                                                                                <  2:511336/140‑1 (MQ=255)                       ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCgg                                                                                                            >  2:1027225/1‑140 (MQ=255)                        tttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCggg                                                                                                           <  1:1000226/140‑1 (MQ=255)                        tttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCggg                                                                                                           <  1:870996/140‑1 (MQ=255)                         ttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGgc                                                                                                          <  2:530469/140‑1 (MQ=255)                          tCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGgcg                                                                                                         <  2:1215348/140‑1 (MQ=255)                          tCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGgcg                                                                                                         <  1:590028/140‑1 (MQ=255)                          tCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGgcg                                                                                                         <  2:79916/140‑1 (MQ=255)                          tCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGgcg                                                                                                         <  1:876018/140‑1 (MQ=255)                             cgcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTa                                                                                                      >  2:705230/1‑140 (MQ=255)                                                    ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGttt                                                                               >  1:424561/1‑140 (MQ=255)                                                      ccTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTg                                                                             <  1:1134310/140‑1 (MQ=255)                                                            tCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCAt                                                                       <  2:1176776/140‑1 (MQ=255)                                                               ccacGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCg                                                                    >  2:665267/1‑140 (MQ=255)                                                                cacGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGa                                                                   <  2:831382/140‑1 (MQ=255)                                                                cacGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGa                                                                   <  1:665267/140‑1 (MQ=255)                                                                cacGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGa                                                                   <  2:509182/140‑1 (MQ=255)                                                                 acGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGAt                                                                  <  1:1129842/140‑1 (MQ=255)                                                                                gtaTAAGAGACAGCCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATGCCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTaaa                                                    >  1:1149622/3‑140 (MQ=255)                                                                                     agagACAGCCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATGCCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAatgcat                                               <  2:1149622/140‑1 (MQ=255)                                                                                             ccGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTc                                       >  1:1039529/1‑140 (MQ=255)                                                                                             ccGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTc                                       >  2:210965/1‑140 (MQ=255)                                                                                              cGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCg                                      >  1:1051416/1‑140 (MQ=255)                                                                                              cGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCg                                      >  2:1213558/1‑140 (MQ=255)                                                                                                     ctctGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCg                               <  1:1213558/140‑1 (MQ=255)                                                                                                     ctctGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCg                               <  1:705230/140‑1 (MQ=255)                                                                                                       ctGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGc                             <  2:1051416/140‑1 (MQ=255)                                                                                                        tGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGcc                            >  2:635176/1‑140 (MQ=255)                                                                                                        tGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGcc                            >  2:507151/1‑140 (MQ=255)                                                                                                        tGCGACATCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGcc                            >  2:844053/1‑140 (MQ=255)                                                                                                               tCGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCACGCCGCTTCGCCTTCCGGCCACCAGaa                     <  1:45836/140‑1 (MQ=255)                                                                                                                cGTATAACGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAAt                    >  1:1206622/1‑140 (MQ=255)                                                                                                                       cGTTACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGc             >  2:1131229/1‑140 (MQ=255)                                                                                                                         ttACTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGa           >  2:930005/1‑140 (MQ=255)                                                                                                                           aCTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAtt         >  2:530736/1‑140 (MQ=255)                                                                                                                           aCTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAtt         >  2:1180135/1‑140 (MQ=255)                                                                                                                           aCTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAtt         >  1:1191706/1‑140 (MQ=255)                                                                                                                            cTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTc        >  2:889311/1‑140 (MQ=255)                                                                                                                            cTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTc        >  2:617571/1‑140 (MQ=255)                                                                                                                            cTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTc        >  2:595264/1‑140 (MQ=255)                                                                                                                            cTGGTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTc        >  2:126264/1‑140 (MQ=255)                                                                                                                               gTTTCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAc     <  2:1206622/140‑1 (MQ=255)                                                                                                                                 ttCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAccc   >  2:243997/1‑140 (MQ=255)                                                                                                                                  tCACATTTACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAAcccc  >  1:339530/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA‑CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCC  >  NC_000913/367379‑367646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑