Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,784	C→T	intergenic (‑40/‑497)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,784	0	C	T	100.0%	245.7 / NA	69	intergenic (‑40/‑497)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/39);  total (30/39)

GCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACA  >  NC_000913/3771647‑3771919                                                                                                                                          |                                                                                                                                        gCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTg                                                                                                                                       <  1:1091560/140‑1 (MQ=255)   aaTCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCATTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGc                                                                                                                                     <  2:853571/140‑1 (MQ=255)   aaTCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGc                                                                                                                                     <  1:942838/140‑1 (MQ=255)           aaGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGaa                                                                                                                             <  1:887897/140‑1 (MQ=255)           aaGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGaa                                                                                                                             <  2:1106617/140‑1 (MQ=255)           aaGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGaa                                                                                                                             >  2:549725/1‑140 (MQ=255)                  gTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTc                                                                                                                      >  2:1175453/1‑140 (MQ=255)                   tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCa                                                                                                                     >  2:987503/1‑140 (MQ=255)                   tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCa                                                                                                                     >  2:541643/1‑140 (MQ=255)                   tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCa                                                                                                                     >  1:1064297/1‑140 (MQ=255)                       gTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCt                                                                                                                 <  2:236663/140‑1 (MQ=255)                        tATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTg                                                                                                                >  2:389455/1‑140 (MQ=255)                          tCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGat                                                                                                              >  1:655832/1‑140 (MQ=255)                          tCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGat                                                                                                              <  2:869336/140‑1 (MQ=255)                           cgcgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGata                                                                                                             >  1:1119531/1‑140 (MQ=255)                             cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                           >  2:266988/1‑140 (MQ=255)                             cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                           >  2:246990/1‑140 (MQ=255)                             cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                           >  1:26244/1‑140 (MQ=255)                                  atTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGAc                                                                                                      <  1:266988/140‑1 (MQ=255)                                  atTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGAc                                                                                                      <  2:596245/140‑1 (MQ=255)                                  atTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGAc                                                                                                      <  1:987503/140‑1 (MQ=255)                                    tCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGCGTCAGGCTGATATTTTGACaa                                                                                                    <  2:868630/140‑1 (MQ=255)                                     ccATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCt                                                                                                                 >  2:170409/1‑126 (MQ=255)                                     ccATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCt                                                                                                                 <  1:170409/126‑1 (MQ=255)                                          aaaaaTCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATcc                                                                                              <  2:26244/140‑1 (MQ=255)                                              aTCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGt                                                                                          >  2:376469/1‑140 (MQ=255)                                                 aCCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTcg                                                                                       <  2:837000/140‑1 (MQ=255)                                                      aaaGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGAtt                                                                                  <  2:599699/140‑1 (MQ=255)                                                      aaaGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGAtt                                                                                  <  1:376469/140‑1 (MQ=255)                                                        aGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATtct                                                                                <  1:246990/140‑1 (MQ=255)                                                         gACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATtctc                                                                               <  1:541643/140‑1 (MQ=255)                                                           cGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGc                                                                             >  2:734181/1‑140 (MQ=255)                                                             aaGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCa                                                                           <  1:1175453/140‑1 (MQ=255)                                                                aTCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATaa                                                                        <  1:1079338/140‑1 (MQ=255)                                                                          ccAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaa                                                              >  1:30067/1‑140 (MQ=255)                                                                            aaCGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaata                                                            >  2:837014/1‑140 (MQ=255)                                                                            aaCGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaata                                                            >  2:400653/1‑140 (MQ=255)                                                                               gcgAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCa                                                                   <  2:17707/130‑1 (MQ=255)                                                                               gcgAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCa                                                                   >  1:17707/1‑130 (MQ=255)                                                                                  aaaTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAAttttt                                                      >  2:675869/1‑140 (MQ=255)                                                                                   aaTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTa                                                     <  1:531368/140‑1 (MQ=255)                                                                                    aTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTag                                                    <  2:655832/140‑1 (MQ=255)                                                                                    aTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTag                                                    <  1:772300/140‑1 (MQ=255)                                                                                           aGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtgat                                             <  2:1064297/140‑1 (MQ=255)                                                                                           aGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtgat                                             >  2:805789/1‑140 (MQ=255)                                                                                             aaaCATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtgatga                                           >  2:316308/1‑140 (MQ=255)                                                                                                 atatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTg                                       <  2:370076/140‑1 (MQ=255)                                                                                                  tatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGc                                      >  1:313926/1‑140 (MQ=255)                                                                                                   atCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCt                                     <  1:517245/140‑1 (MQ=255)                                                                                                           cGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACa                             >  1:303146/1‑140 (MQ=255)                                                                                                           cGGATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACa                             >  1:631788/1‑140 (MQ=255)                                                                                                            ggATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTTATATTTTGACAATAATCCGGGTTCCCGATCCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACAt                            <  2:926628/140‑1 (MQ=255)                                                                                                            ggATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACAt                            <  2:295557/140‑1 (MQ=255)                                                                                                            ggATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACAt                            <  2:538631/140‑1 (MQ=255)                                                                                                             gATTCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATa                           >  1:1183223/1‑140 (MQ=255)                                                                                                               ttCACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAAc                         <  1:675869/140‑1 (MQ=255)                                                                                                                 cACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGa                       <  2:452024/140‑1 (MQ=255)                                                                                                                 cACGTTTATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGa                       <  2:313926/140‑1 (MQ=255)                                                                                                                       tATCAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTAt                 >  2:648978/1‑140 (MQ=255)                                                                                                                          cAGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGAc              <  1:552598/140‑1 (MQ=255)                                                                                                                           aGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg             <  1:642815/140‑1 (MQ=255)                                                                                                                           aGTGTTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg             <  2:1119531/140‑1 (MQ=255)                                                                                                                            gtgtTGTTTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGa            >  2:574262/1‑140 (MQ=255)                                                                                                                              gttgttTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGa            >  2:627258/1‑138 (MQ=255)                                                                                                                              gttgttTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGa            <  1:627258/138‑1 (MQ=255)                                                                                                                              gttgttTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAg          >  2:1191255/1‑140 (MQ=255)                                                                                                                                 gttTTTGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCa       <  2:631788/140‑1 (MQ=255)                                                                                                                                  tttttGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCa       <  2:773607/139‑1 (MQ=255)                                                                                                                                  tttttGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCa       >  1:773607/1‑139 (MQ=255)                                                                                                                                      tGGGTTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACa  <  1:1191255/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACA  >  NC_000913/3771647‑3771919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑