Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,900,259	T→C	S107G (AGC→GGC)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,259	0	T	C	100.0%	309.0 / NA	83	S107G (AGC→GGC)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/41);  total (42/41)

GTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAG  >  NC_000913/3900125‑3900395                                                                                                                                       |                                                                                                                                         gTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTa                                                                                                                                     <  2:431335/140‑1 (MQ=255)  ttGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTac                                                                                                                                    <  2:1047723/140‑1 (MQ=255)  ttGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTac                                                                                                                                    <  2:121156/140‑1 (MQ=255)   tGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTaca                                                                                                                                   >  2:998180/1‑140 (MQ=255)   tGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTaca                                                                                                                                   <  2:389183/140‑1 (MQ=255)   tGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTaca                                                                                                                                   >  2:748019/1‑140 (MQ=255)   tGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTaca                                                                                                                                   >  2:681974/1‑140 (MQ=255)       gTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCAt                                                                                                                               <  1:832248/140‑1 (MQ=255)        tGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATGCTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGt                                                                                                                                      <  1:991961/132‑1 (MQ=255)        tGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGt                                                                                                                                      >  2:991961/1‑132 (MQ=255)          gTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTTGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCaa                                                                                                                            >  1:684394/1‑140 (MQ=255)          gTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCaa                                                                                                                            >  2:1155747/1‑140 (MQ=255)          gTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCaa                                                                                                                            >  2:104932/1‑140 (MQ=255)             aGTTATGGCGATCAAATCACCATGCGCAACAGAACGCATATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACAt                                                                                                                         >  1:71954/1‑140 (MQ=255)               ttATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTg                                                                                                                       >  2:733815/1‑140 (MQ=255)                  tGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTac                                                                                                                                    <  2:396855/124‑1 (MQ=255)                  tGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTac                                                                                                                                    >  1:396855/1‑124 (MQ=255)                  tGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGaaa                                                                                                                    >  2:782252/1‑140 (MQ=255)                  tGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGaaa                                                                                                                    >  2:234843/1‑140 (MQ=255)                   ggCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGaaaa                                                                                                                   <  2:715809/140‑1 (MQ=255)                    gCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGaaaaa                                                                                                                  >  2:1079222/1‑140 (MQ=255)                     cGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGaaaaaa                                                                                                                 <  1:782252/140‑1 (MQ=255)                      gATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAAt                                                                                                                <  1:104932/140‑1 (MQ=255)                       aTCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATa                                                                                                               >  2:231949/1‑140 (MQ=255)                         cAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACt                                                                                                             >  1:578498/1‑140 (MQ=255)                                 cATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTATATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTa                                                                                                         >  2:1125880/1‑136 (MQ=255)                                 cATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTATATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTa                                                                                                         <  1:1125880/136‑1 (MQ=255)                                   tGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGtt                                                                                                   >  1:700269/1‑140 (MQ=255)                                   tGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGtt                                                                                                   <  2:25577/140‑1 (MQ=255)                                    gcgcAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTATACCATCAACATTGAAAAAATACTCGTACAGGTTg                                                                                                  <  2:1086723/140‑1 (MQ=255)                                    gcgcAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTg                                                                                                  <  2:816245/140‑1 (MQ=255)                                     cgcAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGc                                                                                                 >  2:733420/1‑140 (MQ=255)                                     cgcAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGc                                                                                                 >  1:48536/1‑140 (MQ=255)                                       cAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCt                                                                                               >  2:511998/1‑140 (MQ=255)                                       cAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCt                                                                                               <  1:1155747/140‑1 (MQ=255)                                         aCAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCttt                                                                                             <  1:231949/140‑1 (MQ=255)                                         aCAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCttt                                                                                             <  2:421194/140‑1 (MQ=255)                                         aCAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCttt                                                                                             <  2:48536/140‑1 (MQ=255)                                         aCAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCttt                                                                                             <  2:589450/140‑1 (MQ=255)                                         aCAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCACCAACATTGAAAAAATACTCGTACAGGTTGCCttt                                                                                             <  1:234843/140‑1 (MQ=255)                                              aCGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGa                                                                                        <  2:294452/140‑1 (MQ=255)                                               cgcgTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCt                                                                                               >  1:360378/1‑132 (MQ=255)                                               cgcgTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCt                                                                                               <  2:360378/132‑1 (MQ=255)                                               cgcgTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGAt                                                                                       >  1:519422/1‑140 (MQ=255)                                                  gTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAgg                                                                                    >  1:831840/1‑140 (MQ=255)                                                            aaCTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCAc                                                                          <  1:228103/140‑1 (MQ=255)                                                              cTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGgtgt                                                                                 >  1:740864/1‑131 (MQ=255)                                                              cTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGgtgt                                                                                 <  2:740864/131‑1 (MQ=255)                                                              cTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGa                                                                        >  1:377259/1‑140 (MQ=255)                                                              cTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGa                                                                        >  2:114255/1‑140 (MQ=255)                                                               tGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGAc                                                                       <  2:71954/140‑1 (MQ=255)                                                               tGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGAc                                                                       <  1:6705/140‑1 (MQ=255)                                                                 ccTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCa                                                                     >  1:360099/1‑140 (MQ=255)                                                                 ccTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCa                                                                     <  1:733420/140‑1 (MQ=255)                                                                  cTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAg                                                                    >  1:132740/1‑140 (MQ=255)                                                                  cTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAg                                                                    >  2:801709/1‑140 (MQ=255)                                                                       aCCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTcc                                                               <  1:114255/140‑1 (MQ=255)                                                                          aGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGc                                                            <  1:681974/140‑1 (MQ=255)                                                                           gAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCt                                                           <  1:52697/140‑1 (MQ=255)                                                                           gAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCt                                                           >  1:134715/1‑140 (MQ=255)                                                                                       aGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACAATGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTc                                               >  2:728176/1‑140 (MQ=255)                                                                                           aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCa                                              >  1:565224/1‑137 (MQ=255)                                                                                           aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCa                                              <  2:565224/137‑1 (MQ=255)                                                                                           aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCg                                           >  1:118526/1‑140 (MQ=255)                                                                                           aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCg                                           >  1:419004/1‑140 (MQ=255)                                                                                           aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCg                                           <  2:134715/140‑1 (MQ=255)                                                                                                 gcgcTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGaa                                     >  2:126674/1‑140 (MQ=255)                                                                                                 gcgcTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGaa                                     >  1:254336/1‑140 (MQ=255)                                                                                                   gcTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTCCAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAata                                   <  2:118526/140‑1 (MQ=255)                                                                                                      gAGGATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTg                                >  2:167218/1‑140 (MQ=255)                                                                                                        ggATTAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTc                              <  2:132740/140‑1 (MQ=255)                                                                                                           ttAGTCATTGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccgg                           <  2:254336/140‑1 (MQ=255)                                                                                                                  ttGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccgga                    <  1:620773/140‑1 (MQ=255)                                                                                                                   tGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccggaa                   >  2:1150844/1‑140 (MQ=255)                                                                                                                   tGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccggaa                   <  1:998180/140‑1 (MQ=255)                                                                                                                   tGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccggaa                   >  2:204224/1‑140 (MQ=255)                                                                                                                   tGCGGTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccggaa                   >  2:456409/1‑140 (MQ=255)                                                                                                                       gTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcacc               <  2:300206/140‑1 (MQ=255)                                                                                                                       gTGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcacc               <  2:578498/140‑1 (MQ=255)                                                                                                                        tGCCTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcaccc              >  2:177219/1‑140 (MQ=255)                                                                                                                          ccTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcacccac            <  1:177219/140‑1 (MQ=255)                                                                                                                          ccTGTATCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcacccac            <  2:519422/140‑1 (MQ=255)                                                                                                                               aTCAATGCCGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTa       >  1:1222291/1‑140 (MQ=255)                                                                                                                                    tgccgcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACagag  >  2:525081/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         GTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAG  >  NC_000913/3900125‑3900395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑