Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,324	A→G	G64G (GGA→GGG)	wecB →	UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,324	0	A	G	100.0%	293.4 / NA	80	G64G (GGA→GGG)	wecB	UDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/37);  total (43/37)

TTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCA  >  NC_000913/3970187‑3970458                                                                                                                                          |                                                                                                                                       ttGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCa                                                                                                                                      <  2:352372/140‑1 (MQ=255)    gTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCcagggcaggg                                                                                                                                   <  1:1033615/140‑1 (MQ=255)        aTGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTg                                                                                                                               <  1:237788/140‑1 (MQ=255)         tGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGa                                                                                                                              >  1:130227/1‑140 (MQ=255)         tGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGa                                                                                                                              <  2:155013/140‑1 (MQ=255)          gCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGAc                                                                                                                             >  2:362126/1‑140 (MQ=255)           cGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACa                                                                                                                            >  1:879365/1‑140 (MQ=255)           cGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACa                                                                                                                            <  1:362126/140‑1 (MQ=255)               ggCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGa                                                                                                                              >  2:439936/1‑134 (MQ=255)               ggCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGa                                                                                                                              <  1:439936/134‑1 (MQ=255)               ggCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAgaga                                                                                                                        >  2:440910/1‑140 (MQ=255)                     aGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCt                                                                                                                  >  2:214012/1‑140 (MQ=255)                       aTCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGt                                                                                                                <  1:657931/140‑1 (MQ=255)                       aTCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGt                                                                                                                <  1:886320/140‑1 (MQ=255)                       aTCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGt                                                                                                                <  2:1102621/140‑1 (MQ=255)                        tCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTc                                                                                                               <  2:366523/140‑1 (MQ=255)                          cTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGt                                                                                                                <  2:1125619/137‑1 (MQ=255)                          cTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGt                                                                                                                >  1:1125619/1‑137 (MQ=255)                                  gAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGa                                                                                                     <  2:1210096/140‑1 (MQ=255)                                  gAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGa                                                                                                     <  1:214012/140‑1 (MQ=255)                                  gAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGa                                                                                                     <  2:879365/140‑1 (MQ=255)                                               gCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCt                                                                                        >  1:974808/1‑140 (MQ=255)                                                cGTCACTGCGCAGCATCGGGAGATGCTCGATCGGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTa                                                                                       >  1:250605/1‑140 (MQ=255)                                                cGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTa                                                                                       >  1:19710/1‑140 (MQ=255)                                                     cTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCtt                                                                                  >  2:1190839/1‑140 (MQ=255)                                                      tGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTg                                                                                 >  1:1175330/1‑140 (MQ=255)                                                                 gggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCaaa                                                                      <  2:974808/140‑1 (MQ=255)                                                                  ggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGt                                                                           >  2:353040/1‑134 (MQ=255)                                                                  ggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGt                                                                           <  1:353040/134‑1 (MQ=255)                                                                  ggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTc                                                                         <  1:704052/136‑1 (MQ=255)                                                                  ggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTc                                                                         >  2:704052/1‑136 (MQ=255)                                                                      aTGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGa                                                                 <  2:1175330/140‑1 (MQ=255)                                                                       tGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAc                                                                >  2:1198789/1‑140 (MQ=255)                                                                       tGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAc                                                                >  2:634048/1‑140 (MQ=255)                                                                       tGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAc                                                                >  2:845362/1‑140 (MQ=255)                                                                        gCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcg                                                               <  1:206949/140‑1 (MQ=255)                                                                        gCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcg                                                               >  2:476492/1‑140 (MQ=255)                                                                         cTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcgt                                                              >  2:1157955/1‑140 (MQ=255)                                                                         cTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcgt                                                              <  1:230488/140‑1 (MQ=255)                                                                         cTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcgt                                                              >  1:63328/1‑140 (MQ=255)                                                                           cGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcgtcg                                                            <  1:845362/140‑1 (MQ=255)                                                                            gATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGAcgtcgt                                                           >  2:713675/1‑140 (MQ=255)                                                                             aTCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTg                                                          >  1:900066/1‑140 (MQ=255)                                                                             aTCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTg                                                          <  1:634048/140‑1 (MQ=255)                                                                                    gCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTc                                                   >  2:519152/1‑140 (MQ=255)                                                                                     cTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCa                                                  <  2:900066/140‑1 (MQ=255)                                                                                     cTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCa                                                  <  1:519152/140‑1 (MQ=255)                                                                                        aaaCTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACgg                                               <  1:1198789/140‑1 (MQ=255)                                                                                         aaCTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGc                                              <  2:130227/140‑1 (MQ=255)                                                                                                       gTACCTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgac                                >  1:257590/1‑140 (MQ=255)                                                                                                          ccTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCt                             >  1:874029/1‑140 (MQ=255)                                                                                                          ccTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCt                             <  2:874029/140‑1 (MQ=255)                                                                                                           cTGACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTg                            <  2:257590/140‑1 (MQ=255)                                                                                                             gACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGc                          >  2:488143/1‑140 (MQ=255)                                                                                                             gACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGc                          >  1:954903/1‑140 (MQ=255)                                                                                                             gACTACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGc                          >  1:767796/1‑140 (MQ=255)                                                                                                                tACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAc                       <  1:388901/140‑1 (MQ=255)                                                                                                                tACGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAc                       <  1:440910/140‑1 (MQ=255)                                                                                                                  cGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCT‑AAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCt                             >  1:251323/1‑131 (MQ=255)                                                                                                                  cGATCTCAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCT‑AAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCt                             <  2:251323/131‑1 (MQ=255)                                                                                                                        cAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTgg               >  2:504713/1‑140 (MQ=255)                                                                                                                        cAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTgg               >  1:619309/1‑140 (MQ=255)                                                                                                                        cAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTgg               >  1:813620/1‑140 (MQ=255)                                                                                                                        cAACATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTgg               >  1:86096/1‑140 (MQ=255)                                                                                                                         aaCATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggc              >  2:184052/1‑140 (MQ=255)                                                                                                                         aaCATAATGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggc              <  2:86096/140‑1 (MQ=255)                                                                                                                              aaTGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGt         >  1:876959/1‑140 (MQ=255)                                                                                                                               aTGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGtt        >  2:1141300/1‑140 (MQ=255)                                                                                                                               aTGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGtt        >  2:462876/1‑140 (MQ=255)                                                                                                                                tGCAGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGttt       >  1:1001288/1‑140 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggc              >  1:65795/1‑130 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggc              <  2:65795/130‑1 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt    <  1:504713/140‑1 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt    >  2:409027/1‑140 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt    <  2:677154/140‑1 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt    <  2:813620/140‑1 (MQ=255)                                                                                                                                   aGCCAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt    >  2:837875/1‑140 (MQ=255)                                                                                                                                     ccAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa  >  1:885014/1‑140 (MQ=255)                                                                                                                                     ccAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa  <  1:488143/140‑1 (MQ=255)                                                                                                                                     ccAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa  <  1:409027/140‑1 (MQ=255)                                                                                                                                     ccAGGGCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa  >  2:472873/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCA  >  NC_000913/3970187‑3970458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑