Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,422	G→A	Y467Y (TAC→TAT)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,422	0	G	A	100.0%	224.7 / NA	64	Y467Y (TAC→TAT)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/27);  total (37/27)

AGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATGTAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATCGTACG  >  NC_000913/4084289‑4084556                                                                                                                                      |                                                                                                                                       agGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCt                                                                                                                                  >  1:214226/1‑140 (MQ=255)  ggCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTg                                                                                                                                 >  1:656751/1‑140 (MQ=255)  ggCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTg                                                                                                                                 <  2:632916/140‑1 (MQ=255)     cTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCa                                                                                                                              >  1:403985/1‑140 (MQ=255)        catcatAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGc                                                                                                                           >  1:174010/1‑140 (MQ=255)             tAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGc                                                                                                                      >  2:977327/1‑140 (MQ=255)              agagACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCg                                                                                                                     >  1:534519/1‑140 (MQ=255)                  aCGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGAcag                                                                                                                 >  1:4538/1‑140 (MQ=255)                   cGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGAGTGTTGGCGGTAAGGGAGGTTTGCAGATCGGTCTGCCTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGAcagc                                                                                                                >  2:1197717/1‑140 (MQ=255)                     aagaaTTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGa                                                                                                                    <  2:740821/134‑1 (MQ=255)                     aagaaTTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGa                                                                                                                    >  1:740821/1‑134 (MQ=255)                           ttCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAg                                                                                                        <  2:174010/140‑1 (MQ=255)                                  aGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTc                                                                                                 <  2:942723/140‑1 (MQ=255)                                    ttGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCCGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAg                                                                                               <  1:235062/140‑1 (MQ=255)                                       ccccAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGccc                                                                                            <  2:656751/140‑1 (MQ=255)                                         ccAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCt                                                                                          <  2:4538/140‑1 (MQ=255)                                          cAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTg                                                                                         >  2:287553/1‑140 (MQ=255)                                             tagtTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAat                                                                                      >  1:306942/1‑140 (MQ=255)                                             tagtTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAat                                                                                      >  1:1084040/1‑140 (MQ=255)                                                 ttACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCCGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTg                                                                                  >  2:1209931/1‑140 (MQ=255)                                                  tACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGccc                                                                                            >  1:452427/1‑129 (MQ=255)                                                  tACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGccc                                                                                            <  2:452427/129‑1 (MQ=255)                                                    ccTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAg                                                                               <  1:356684/140‑1 (MQ=255)                                                     cTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGTGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGt                                                                              >  1:675774/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTTAGCCCCTGAATATTGGAGTGa                                                                            >  2:546675/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  1:763812/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  1:1075159/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  1:30981/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  2:290969/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  2:869846/1‑140 (MQ=255)                                                       ggCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGa                                                                            >  2:367940/1‑140 (MQ=255)                                                        gCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGAc                                                                           <  1:287553/140‑1 (MQ=255)                                                             ccAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCgcgc                                                                      <  1:958968/140‑1 (MQ=255)                                                                      gTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTa                                                             >  2:672626/1‑140 (MQ=255)                                                                                   ggCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTg                                                <  2:306942/140‑1 (MQ=255)                                                                                    gCGGTCAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGc                                               <  2:1084040/140‑1 (MQ=255)                                                                                       gTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCAt                                            <  1:290969/140‑1 (MQ=255)                                                                                         aaGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGc                                               >  2:713630/1‑135 (MQ=255)                                                                                         aaGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGc                                               <  1:713630/135‑1 (MQ=255)                                                                                         aaGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATcc                                          <  1:869846/140‑1 (MQ=255)                                                                                           ggtaggtTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGTCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATcccc                                        >  2:995218/1‑140 (MQ=255)                                                                                           ggtaggtTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATcccc                                        >  1:662327/1‑140 (MQ=255)                                                                                            gtaggtTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGTCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCa                                       <  1:995218/140‑1 (MQ=255)                                                                                              aggtTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCata                                     >  2:1047834/1‑140 (MQ=255)                                                                                                gtTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATAtt                                   >  1:314758/1‑140 (MQ=255)                                                                                                    gCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTg                                                >  1:979469/1‑123 (MQ=255)                                                                                                    gCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTg                                                <  2:979469/123‑1 (MQ=255)                                                                                                      aGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCAt                                            <  2:187339/125‑1 (MQ=255)                                                                                                      aGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCAt                                            >  1:187339/1‑125 (MQ=255)                                                                                                      aGATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGAGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGag                             <  2:214226/140‑1 (MQ=255)                                                                                                       gATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGagc                            <  2:314758/140‑1 (MQ=255)                                                                                                       gATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGagc                            <  2:763812/140‑1 (MQ=255)                                                                                                       gATCGGTCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGagc                            <  2:67005/140‑1 (MQ=255)                                                                                                             tCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGagc                            >  1:1154113/1‑134 (MQ=255)                                                                                                             tCTGCTTCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGagc                            <  2:1154113/134‑1 (MQ=255)                                                                                                                  ttCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGat                 >  2:572705/1‑140 (MQ=255)                                                                                                                  ttCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGat                 >  1:633009/1‑140 (MQ=255)                                                                                                                  ttCTCGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGat                 <  2:403985/140‑1 (MQ=255)                                                                                                                      cGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGatcatc             <  1:672626/140‑1 (MQ=255)                                                                                                                      cGCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGatcatc             >  1:1101528/1‑140 (MQ=255)                                                                                                                       gCTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCg            >  2:484848/1‑140 (MQ=255)                                                                                                                        cTTGGCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGc           <  1:546675/140‑1 (MQ=255)                                                                                                                           ggCAGCGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCA‑GGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATc       >  2:1156691/1‑140 (MQ=255)                                                                                                                                cGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATCGTa    <  1:371408/139‑1 (MQ=255)                                                                                                                                cGTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATCGTa    >  2:371408/1‑139 (MQ=255)                                                                                                                                 gTCATATAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATCGTACg  >  2:966184/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGTTTGCAGATCGGTCTGCTTCTCGCTTGGCAGCGTCATGTAACCTGGCAGGCTCTGCGACAGCAGCCCCAGGTCCGTCAGCCCCTGAATATTGGAGTGACCGCGCAGGGCGTTAACGCCGCCGCCTGCCATCCCCATATTGCCGAGCAGCAGCTGGATCATCGCCATCGTACG  >  NC_000913/4084289‑4084556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑