Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,099,985	A→G	C181R (TGC→CGC)	rhaT ←	rhamnose/lyxose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,099,985	0	A	G	100.0%	215.6 / NA	63	C181R (TGC→CGC)	rhaT	rhamnose/lyxose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/21);  total (42/21)

GCGCCGCCGCCCATGATGACAACATAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATG  >  NC_000913/4099852‑4100121                                                                                                                                      |                                                                                                                                         gcgcCGCCGCCCATGATGACAACATAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCAt                                                                                                                                       >  1:564043/1‑137 (MQ=255) gcgcCGCCGCCCATGATGACAACATAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCAt                                                                                                                                       <  2:564043/137‑1 (MQ=255)  cgccgccgcCCATGATGACAACATAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCAcc                                                                                                                                   >  2:505687/1‑140 (MQ=255)                    caacaTAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCa                                                                                                                         >  2:632053/1‑132 (MQ=255)                    caacaTAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCa                                                                                                                         <  1:632053/132‑1 (MQ=255)                    caacaTAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCtttt                                                                                                                 >  1:1042359/1‑140 (MQ=255)                        aTAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCa                                                                                                             >  1:45248/1‑140 (MQ=255)                         tAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAg                                                                                                            >  1:400471/1‑140 (MQ=255)                         tAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAg                                                                                                            >  2:494517/1‑140 (MQ=255)                            cTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGAtt                                                                                                         >  2:233908/1‑140 (MQ=255)                               ggCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGaa                                                                                                      <  2:969516/140‑1 (MQ=255)                                 cAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCtttttt                                                                                                               >  1:430150/1‑129 (MQ=255)                                 cAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCtttttt                                                                                                               <  2:430150/129‑1 (MQ=255)                                 cAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAAct                                                                                                    >  1:593980/1‑140 (MQ=255)                                    agCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCtt                                                                                                 <  1:233908/140‑1 (MQ=255)                                    agCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCtt                                                                                                 >  2:222080/1‑140 (MQ=255)                                         cATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCt                                                                                            >  1:661549/1‑140 (MQ=255)                                                tGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCttt                                                                                          <  1:617056/135‑1 (MQ=255)                                                tGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCttt                                                                                          >  2:617056/1‑135 (MQ=255)                                                tGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGc                                                                                     <  2:560369/140‑1 (MQ=255)                                                  gATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGccc                                                                                   >  1:1170340/1‑140 (MQ=255)                                                   aTCGACGCCAAGTGCGGCAGCGGCTTCATTCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCa                                                                                  >  1:394432/1‑140 (MQ=255)                                                   aTCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCa                                                                                  >  2:179356/1‑140 (MQ=255)                                                   aTCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCa                                                                                  >  2:505120/1‑140 (MQ=255)                                                    tCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCAt                                                                                 >  1:143987/1‑140 (MQ=255)                                                     cGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTAAGATTGAACTCTTCGGCTTTAATGCCCATc                                                                                >  2:517333/1‑140 (MQ=255)                                                       aCGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCtt                                                                              >  2:237422/1‑140 (MQ=255)                                                       aCGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCtt                                                                              >  2:905100/1‑140 (MQ=255)                                                       aCGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCtt                                                                              >  2:35877/1‑140 (MQ=255)                                                       aCGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCtt                                                                              >  2:406663/1‑140 (MQ=255)                                                       aCGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCtt                                                                              >  1:996879/1‑140 (MQ=255)                                                        cGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTg                                                                             >  2:310021/1‑140 (MQ=255)                                                        cGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTg                                                                             >  1:154218/1‑140 (MQ=255)                                                        cGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTg                                                                             >  1:897510/1‑140 (MQ=255)                                                         gCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTgc                                                                            <  1:654781/140‑1 (MQ=255)                                                         gCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTgc                                                                            <  2:593980/140‑1 (MQ=255)                                                         gCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTGGCGGCGTCCATCGAAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTgc                                                                            <  2:1061594/140‑1 (MQ=255)                                                          ccAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTgcg                                                                           <  1:905100/140‑1 (MQ=255)                                                             aGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGctc                                                                        <  1:636496/140‑1 (MQ=255)                                                              gTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGctct                                                                       >  2:460244/1‑140 (MQ=255)                                                              gTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGctct                                                                       <  1:253927/140‑1 (MQ=255)                                                                         gCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGccc                                                            >  2:392936/1‑140 (MQ=255)                                                                               tGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGcgcg                                                      <  1:392936/140‑1 (MQ=255)                                                                                gCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGa                                                     <  2:996879/140‑1 (MQ=255)                                                                                 cATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAg                                                    <  2:394432/140‑1 (MQ=255)                                                                                 cATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTAAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAg                                                    <  1:517333/140‑1 (MQ=255)                                                                                     ggTTTAGCGGCGTCCTTCGCAAAGGACATGCGGGCAGAGAAAATGCGGCGCAACACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGAGCTCTTTCAACTGCCCGGCGCGAGTTAc                                                <  2:1081078/140‑1 (MQ=255)                                                                                      gTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACa                                               <  1:310021/140‑1 (MQ=255)                                                                                          tGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATcc                                           <  2:1042359/140‑1 (MQ=255)                                                                                          tGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATcc                                           >  1:1159460/1‑140 (MQ=255)                                                                                            cggcgTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGTGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCt                                         >  1:1081078/1‑140 (MQ=255)                                                                                                 ttCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGcc                                    >  2:378522/1‑140 (MQ=255)                                                                                                 ttCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGcc                                    >  2:546397/1‑140 (MQ=255)                                                                                                   cATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCaa                                  >  1:410262/1‑140 (MQ=255)                                                                                                        cAAAGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAgc                             <  2:1159460/140‑1 (MQ=255)                                                                                                        aaaaGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAgc                             <  1:911399/139‑1 (MQ=255)                                                                                                           aGGACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCCCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGcc                          >  1:664263/1‑140 (MQ=255)                                                                                                            ggACATCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGcca                         >  1:657720/1‑140 (MQ=255)                                                                                                               caccccGGCAGAAAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGccacca                      <  1:369975/137‑1 (MQ=255)                                                                                                                aTCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAg                     >  1:1026068/1‑140 (MQ=255)                                                                                                                aTCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAg                     >  1:147967/1‑140 (MQ=255)                                                                                                                aTCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAg                     >  1:281550/1‑140 (MQ=255)                                                                                                                aTCCCGGCAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAg                     >  1:952389/1‑140 (MQ=255)                                                                                                                       cAGAGAAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCg              <  2:147967/140‑1 (MQ=255)                                                                                                                         gagaAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAg            <  2:897510/140‑1 (MQ=255)                                                                                                                          agaAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCATACGCCAATCAGCGCCACCAGAACGCCGAGc           >  2:932643/1‑140 (MQ=255)                                                                                                                          agaAAATGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCCCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGc           <  2:664263/140‑1 (MQ=255)                                                                                                                            aaaaTGCCGCGCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCaa         >  2:631034/1‑140 (MQ=255)                                                                                                                                   cgcgCATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATg  <  2:281550/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GCGCCGCCGCCCATGATGACAACATAGCTTGGCAGAGCGACATACAGTGGATCGACGCCAAGTGCGGCAGCGGCTTCATGCATCGGTTTTGCGGCGTTCATCGCAAAGGACATCCCGGCAGAGAAAATGCCGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATG  >  NC_000913/4099852‑4100121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑