Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,076	C→T	E383K (GAG→AAG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,076	0	C	T	100.0%	224.9 / NA	64	E383K (GAG→AAG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/37);  total (27/37)

CGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTT  >  NC_000913/4115941‑4116210                                                                                                                                        |                                                                                                                                       cGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGc                                                                                                                                    <  2:1056293/140‑1 (MQ=255)     aaTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTcg                                                                                                                                <  1:1125576/140‑1 (MQ=255)     aaTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTcg                                                                                                                                <  2:56234/140‑1 (MQ=255)     aaTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTcg                                                                                                                                <  1:209774/140‑1 (MQ=255)     aaTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTcg                                                                                                                                <  1:997546/140‑1 (MQ=255)      atatCGAACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTcgc                                                                                                                               <  1:382602/140‑1 (MQ=255)          cGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGt                                                                                                                           <  1:413008/140‑1 (MQ=255)            gACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGtat                                                                                                                         <  1:646829/140‑1 (MQ=255)              cTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATaa                                                                                                                       <  2:191162/140‑1 (MQ=255)                 gAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAAtgt                                                                                                                    <  1:772090/140‑1 (MQ=255)                       cATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGt                                                                                                                           >  2:771760/1‑127 (MQ=255)                       cATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGt                                                                                                                           <  1:771760/127‑1 (MQ=255)                           aGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGtt                                                                                                          <  2:695894/140‑1 (MQ=255)                                    tttGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGtt                                                                                                                >  1:824690/1‑125 (MQ=255)                                    tttGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGtt                                                                                                                <  2:824690/125‑1 (MQ=255)                                       gCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCa                                                                                                        >  2:907787/1‑130 (MQ=255)                                       gCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCa                                                                                                        <  1:907787/130‑1 (MQ=255)                                       gCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGt                                                                                              >  2:282078/1‑140 (MQ=255)                                           cTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGa                                                                                          >  1:283516/1‑140 (MQ=255)                                            tGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGAc                                                                                         >  1:327152/1‑140 (MQ=255)                                              cgcCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCg                                                                                       >  2:602490/1‑140 (MQ=255)                                                ccaccaTCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGaa                                                                                     >  2:368830/1‑140 (MQ=255)                                                    caTCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATc                                                                                 >  2:1112465/1‑140 (MQ=255)                                                           gcgcAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCcgc                                                                          <  1:1158849/140‑1 (MQ=255)                                                                 ggCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCAt                                                                    >  2:542036/1‑140 (MQ=255)                                                                  gCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                                   <  2:283516/140‑1 (MQ=255)                                                                    gTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                                 <  1:602490/140‑1 (MQ=255)                                                                    gTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                                 <  1:175343/140‑1 (MQ=255)                                                                    gTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                                 <  1:1073070/140‑1 (MQ=255)                                                                     tGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACgg                                                                <  2:327152/140‑1 (MQ=255)                                                                     tGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACgg                                                                >  2:920367/1‑140 (MQ=255)                                                                        aGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCACGCATACGGGTc                                                             <  1:1112465/140‑1 (MQ=255)                                                                           cGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                                          >  2:866975/1‑140 (MQ=255)                                                                           cGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                                          >  2:438095/1‑140 (MQ=255)                                                                                 ccAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                                    <  1:282078/140‑1 (MQ=255)                                                                                    gagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgcgc                                                 >  1:784864/1‑140 (MQ=255)                                                                                    gagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgcgc                                                 >  2:1110588/1‑140 (MQ=255)                                                                                     agTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCa                                                >  1:548362/1‑140 (MQ=255)                                                                                       tCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAcc                                              <  2:784864/140‑1 (MQ=255)                                                                                        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAccc                                             >  2:876301/1‑140 (MQ=255)                                                                                        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAccc                                             >  1:565277/1‑140 (MQ=255)                                                                                        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAccc                                             >  1:1162188/1‑140 (MQ=255)                                                                                          gCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAg                                           >  2:871291/1‑140 (MQ=255)                                                                                            cTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGcc                                         >  2:479360/1‑140 (MQ=255)                                                                                                        cAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                             <  2:1102682/140‑1 (MQ=255)                                                                                                        cAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                             <  1:479360/140‑1 (MQ=255)                                                                                                        cAGCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                             <  1:438095/140‑1 (MQ=255)                                                                                                          gCACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGa                           <  1:866975/140‑1 (MQ=255)                                                                                                           cACGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGaa                          <  1:368830/140‑1 (MQ=255)                                                                                                            aCGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc                         <  1:871291/140‑1 (MQ=255)                                                                                                            aCGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc                         <  1:766579/140‑1 (MQ=255)                                                                                                             cGTCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAcc                        <  1:542036/140‑1 (MQ=255)                                                                                                               tCACGCGTCTGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACcac                      <  2:62347/140‑1 (MQ=255)                                                                                                                        tGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATaca                 <  1:292280/136‑1 (MQ=255)                                                                                                                        tGATAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATaca                 >  2:292280/1‑136 (MQ=255)                                                                                                                          aTAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt           <  2:548362/140‑1 (MQ=255)                                                                                                                          aTAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt           >  2:363721/1‑140 (MQ=255)                                                                                                                          aTAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt           >  1:1121323/1‑140 (MQ=255)                                                                                                                          aTAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt           <  2:1121323/140‑1 (MQ=255)                                                                                                                          aTAAGCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt           <  1:1110588/140‑1 (MQ=255)                                                                                                                              gCAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGgt       <  1:920367/140‑1 (MQ=255)                                                                                                                               cAATAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGgtg      >  2:1173274/1‑140 (MQ=255)                                                                                                                                 aTAGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGtt    >  1:1198265/1‑140 (MQ=255)                                                                                                                                   aGACTTCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGtttt  >  1:195409/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTT  >  NC_000913/4115941‑4116210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑