Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,126,671	A→G	V47A (GTA→GCA)	priA ←	primosome factor N'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,126,671	0	A	G	100.0%	366.7 / NA	99	V47A (GTA→GCA)	priA	primosome factor N'
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/46);  total (53/46)

AATCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATACCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAACGTGGGCAAC  >  NC_000913/4126535‑4126804                                                                                                                                         |                                                                                                                                        aaTCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCca                                                                                                                                      <  2:1183723/140‑1 (MQ=255) aaTCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCca                                                                                                                                      <  2:928263/140‑1 (MQ=255) aaTCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCca                                                                                                                                      <  2:281162/140‑1 (MQ=255)   tCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCcaca                                                                                                                                    <  2:45527/140‑1 (MQ=255)       atgatgatAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATcc                                                                                                                                >  2:1107604/1‑140 (MQ=255)       atgatgatAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATcc                                                                                                                                >  1:1155995/1‑140 (MQ=255)           tgatAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGAt                                                                                                                            <  1:880639/140‑1 (MQ=255)            gatAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATg                                                                                                                           <  2:241164/140‑1 (MQ=255)            gatAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATg                                                                                                                           <  1:1218371/140‑1 (MQ=255)                 tAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTc                                                                                                                      >  2:351196/1‑140 (MQ=255)                   aTCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACAACGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCct                                                                                                                    >  2:418843/1‑140 (MQ=255)                     cTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCctgc                                                                                                                  >  2:1149402/1‑140 (MQ=255)                      tGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTATCTGATGCCACAATCCCGATGCGCTCcttct                                                                                                                 >  2:750419/1‑140 (MQ=255)                      tGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCctgct                                                                                                                 >  1:470570/1‑140 (MQ=255)                      tGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCctgct                                                                                                                 >  2:784823/1‑140 (MQ=255)                        ccgccCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGt                                                                                                               >  1:240843/1‑140 (MQ=255)                           cccATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTg                                                                                                            >  1:929965/1‑140 (MQ=255)                             cATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGcc                                                                                                          >  1:1081782/1‑140 (MQ=255)                              aTAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCa                                                                                                         <  2:518841/140‑1 (MQ=255)                                       aTCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGgca‑‑cg                                                                                              >  2:935884/1‑140 (MQ=255)                                         cGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGgca‑‑cgcg                                                                                            <  1:951541/140‑1 (MQ=255)                                          gCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGgca‑‑cgcgc                                                                                           <  1:935884/140‑1 (MQ=255)                                          gCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGgca‑‑cgcgc                                                                                           >  1:554094/1‑140 (MQ=255)                                              gACGGAGTGAGTAAACAACGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGgca‑‑cgcgcacgc                                                                                       <  1:418843/140‑1 (MQ=255)                                               aCGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGca‑‑cgcgcacgcg                                                                                      <  2:1155995/140‑1 (MQ=255)                                                   agtgagtAAACATCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGacac                                                                                  >  1:1049360/1‑140 (MQ=255)                                                   agtgagtAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGacac                                                                                  >  2:53520/1‑140 (MQ=255)                                                    gtgagtAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACAcc                                                                                 <  2:1107641/140‑1 (MQ=255)                                                           aaCACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCttt                                                                          <  2:554094/140‑1 (MQ=255)                                                             cacCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        >  2:800094/1‑140 (MQ=255)                                                             cacCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        >  2:349926/1‑140 (MQ=255)                                                             cacCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        <  1:351196/140‑1 (MQ=255)                                                             cacCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTCGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        >  1:650040/1‑140 (MQ=255)                                                              acCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        <  1:1042687/139‑1 (MQ=255)                                                              acCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTaa                                                                        >  2:1042687/1‑139 (MQ=255)                                                                 ggCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCACGCGCGCACGCGACACCCAGCTTTAAcc                                                                      >  2:1212345/1‑140 (MQ=255)                                                                 ggCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGt                                                                    >  2:7763/1‑140 (MQ=255)                                                                 ggCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGt                                                                    >  2:1006183/1‑140 (MQ=255)                                                                 ggCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGt                                                                    >  1:853576/1‑140 (MQ=255)                                                                  gCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTc                                                                   >  2:207932/1‑140 (MQ=255)                                                                   cTCACTATCCAGCGCTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCa                                                                  >  1:579195/1‑140 (MQ=255)                                                                   cTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCACGCGCGCACGCGACACCCAGCTTTAACCGt                                                                    <  1:1212345/140‑1 (MQ=255)                                                                   cTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCa                                                                  <  1:207932/140‑1 (MQ=255)                                                                     cACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATg                                                                >  1:925361/1‑140 (MQ=255)                                                                        tATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCt                                                             <  2:658199/140‑1 (MQ=255)                                                                        tATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCt                                                             <  1:349926/140‑1 (MQ=255)                                                                              gCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgc                                                       <  1:1149402/140‑1 (MQ=255)                                                                               cACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgca                                                      >  1:228072/1‑140 (MQ=255)                                                                                aCTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcag                                                     >  1:880704/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCGTGCCTTCTGgcagca                                                   >  2:345781/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcagca                                                   >  2:413537/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcagca                                                   >  2:705024/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcagca                                                   >  2:103093/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcagca                                                   >  1:618875/1‑140 (MQ=255)                                                                                  ttCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgcagca                                                   >  2:111584/1‑140 (MQ=255)                                                                                   tCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGcagcag                                                  <  1:111584/140‑1 (MQ=255)                                                                                   tCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGcagcag                                                  <  1:1201293/140‑1 (MQ=255)                                                                                   tCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGcagcag                                                  <  1:705024/140‑1 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTc                                                           >  2:907626/1‑130 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTc                                                           <  1:907626/130‑1 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgca                                                      <  1:411562/135‑1 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGgca                                                      >  2:411562/1‑135 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 >  1:124803/1‑140 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 >  2:1198635/1‑140 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 >  2:239272/1‑140 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 <  2:618875/140‑1 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 >  1:163365/1‑140 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 <  1:750419/140‑1 (MQ=255)                                                                                    cGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGa                                                 >  1:774752/1‑140 (MQ=255)                                                                                           cGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCaa                                          <  1:239272/140‑1 (MQ=255)                                                                                           cGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCaa                                          <  1:441481/140‑1 (MQ=255)                                                                                            gCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGAAGCAGATAGTCaaa                                         >  2:754734/1‑140 (MQ=255)                                                                                             cTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAg                                        >  2:52743/1‑140 (MQ=255)                                                                                                  aGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACg                                   <  1:800094/140‑1 (MQ=255)                                                                                                     tCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAgg                                >  1:68929/1‑140 (MQ=255)                                                                                                       aTTGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGaa                              <  1:706395/140‑1 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAgg                                >  1:672523/1‑137 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAgg                                <  2:672523/137‑1 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAg                             >  2:464806/1‑140 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAg                             <  2:880704/140‑1 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAg                             <  2:774752/140‑1 (MQ=255)                                                                                                        ttGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCC‑AACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGCACGAGGAAGc                            >  2:436525/1‑140 (MQ=255)                                                                                                         tGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACg                                   <  1:445192/133‑1 (MQ=255)                                                                                                         tGAGCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACg                                   >  2:445192/1‑133 (MQ=255)                                                                                                            gCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCGTGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGa                         <  1:345781/140‑1 (MQ=255)                                                                                                            gCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGa                         <  1:381226/140‑1 (MQ=255)                                                                                                            gCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGa                         <  1:413537/140‑1 (MQ=255)                                                                                                            gCGGCAGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGa                         <  1:464806/140‑1 (MQ=255)                                                                                                                 aGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACggg                    >  1:158558/1‑140 (MQ=255)                                                                                                                 aGTTCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACggg                    <  2:579195/140‑1 (MQ=255)                                                                                                                   ttCGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGggca                  >  2:903772/1‑140 (MQ=255)                                                                                                                     cGCTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGggcaag                <  2:337943/140‑1 (MQ=255)                                                                                                                       cTGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGggcaaggc              <  2:835247/140‑1 (MQ=255)                                                                                                                        tGGCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGggcaaggca             >  2:527428/1‑140 (MQ=255)                                                                                                                         ggCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGggcaaggcaa            >  2:958198/1‑140 (MQ=255)                                                                                                                          gCATCGCTAACTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAAc           >  1:723759/1‑140 (MQ=255)                                                                                                                                  aaCTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAACGTGGGCaa   <  1:1006183/140‑1 (MQ=255)                                                                                                                                   aCTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAACGTGGGCAAc  <  2:240843/140‑1 (MQ=255)                                                                                                                                   aCTGATGCCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAACGTGGGCAAc  <  1:784823/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AATCGGATGATGATAGTAATCTGCCGCCCATAGCAGCAATCGCCAGACGGAGTGAGTAAACACCGGCTCACTATCCAGCACTTCGACTACCGCTTTTAGCTCATTGAGCGGCAGTTCGCTGGCATCGCTAACTGATACCACAATCCCGATGCGCTCCTGCTGTTTGCCAAACGGCA‑‑CGCGCACGCGACACCCAGCTTTAACCGTCATGCCTTCTGGCAGCAGATAGTCAAAGGTACGAGGAAGCGGAACGGGCAAGGCAACGTGGGCAAC  >  NC_000913/4126535‑4126804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑