Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,286	C→T	R11H (CGC→CAC)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,286	0	C	T	100.0%	221.0 / NA	65	R11H (CGC→CAC)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/42);  total (23/42)

GCGCCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGCGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTAAACATTGCGG  >  NC_000913/4148151‑4148419                                                                                                                                        |                                                                                                                                      gcgcCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGga                                                                                                                                   <  2:456949/140‑1 (MQ=255)      ggTATCAACAAACTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCa                                                                                                                              >  2:1007764/1‑140 (MQ=255)       gTATCAACAAACTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAg                                                                                                                             <  1:478208/140‑1 (MQ=255)             acaaACTCCCCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCt                                                                                                                       <  2:174920/140‑1 (MQ=255)               aaaCTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGa                                                                                                                     <  2:1151551/140‑1 (MQ=255)               aaaCTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGa                                                                                                                     <  1:186664/140‑1 (MQ=255)                 aCTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcg                                                                                                                   <  1:1007764/140‑1 (MQ=255)                 aCTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcg                                                                                                                   <  1:537025/140‑1 (MQ=255)                 aCTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcg                                                                                                                   <  2:941422/140‑1 (MQ=255)                  cTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgt                                                                                                                  <  2:561752/140‑1 (MQ=255)                  cTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgt                                                                                                                  >  2:5605/1‑140 (MQ=255)                    cACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGGTCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgtcg                                                                                                                <  2:826021/140‑1 (MQ=255)                     aCCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgtcgt                                                                                                               <  2:1000557/140‑1 (MQ=255)                        ttCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGAt                                                                                                            <  2:296351/140‑1 (MQ=255)                          ccAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATAc                                                                                                          >  2:1064005/1‑140 (MQ=255)                          ccAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATAc                                                                                                          <  1:1101140/140‑1 (MQ=255)                            aGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACAt                                                                                                        >  1:1196238/1‑140 (MQ=255)                             gCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAAATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATg                                                                                                       >  2:809912/1‑140 (MQ=255)                               acaATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGtt                                                                                                     <  2:1211147/140‑1 (MQ=255)                               acaATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGtt                                                                                                     <  1:14259/140‑1 (MQ=255)                               acaATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGtt                                                                                                     <  1:1064005/140‑1 (MQ=255)                                caATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  2:900448/140‑1 (MQ=255)                                caATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  1:311932/140‑1 (MQ=255)                                caATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  2:322735/140‑1 (MQ=255)                                caATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  2:768347/140‑1 (MQ=255)                                caATTTCCAGCCGTGGAAAATCGACCGGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  2:1176394/140‑1 (MQ=255)                                 aaTTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgtcgt                                                                                                               >  1:310030/1‑128 (MQ=255)                                 aaTTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGAcgtcgt                                                                                                               <  2:310030/128‑1 (MQ=255)                                   tttCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTc                                                                                                 >  1:961927/1‑140 (MQ=255)                                    ttCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTcc                                                                                                <  1:908732/140‑1 (MQ=255)                                      ccAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCt                                                                                              <  2:961927/140‑1 (MQ=255)                                       cAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCtt                                                                                             <  1:1146248/140‑1 (MQ=255)                                       cAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCtt                                                                                             <  1:434531/140‑1 (MQ=255)                                        aGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTa                                                                                            <  2:631655/140‑1 (MQ=255)                                          ccGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTAt                                                                                                   >  2:281364/1‑131 (MQ=255)                                          ccGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTAt                                                                                                   <  1:281364/131‑1 (MQ=255)                                           cGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATa                                                                                                           >  1:241793/1‑122 (MQ=255)                                           cGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATa                                                                                                           <  2:241793/122‑1 (MQ=255)                                             tGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    <  1:774739/127‑1 (MQ=255)                                             tGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTa                                                                                                    >  2:774739/1‑127 (MQ=255)                                               gAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCt                                                                                              >  2:1157356/1‑131 (MQ=255)                                               gAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCt                                                                                              <  1:1157356/131‑1 (MQ=255)                                                                          gCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCattat                                                          <  1:248628/140‑1 (MQ=255)                                                                                ggCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    >  1:334184/1‑140 (MQ=255)                                                                                ggCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    >  1:394859/1‑140 (MQ=255)                                                                                ggCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    >  2:1056981/1‑140 (MQ=255)                                                                                ggCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    >  2:878459/1‑140 (MQ=255)                                                                                    gTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGc                                                <  1:433894/140‑1 (MQ=255)                                                                                       aCTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCAc                                             <  1:254688/140‑1 (MQ=255)                                                                                           ctgGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCa                                              >  1:490916/1‑135 (MQ=255)                                                                                           ctgGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCa                                              <  2:490916/135‑1 (MQ=255)                                                                                           ctgGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGa                                         <  1:5605/140‑1 (MQ=255)                                                                                           ctgGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGa                                         >  1:1050263/1‑140 (MQ=255)                                                                                            tgGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGaa                                        <  1:599778/140‑1 (MQ=255)                                                                                               cAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAg                                     >  2:270859/1‑140 (MQ=255)                                                                                               cAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAg                                     >  2:14000/1‑140 (MQ=255)                                                                                                  aGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    <  2:1034401/122‑1 (MQ=255)                                                                                                  aGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTaaaaa                                                    >  1:1034401/1‑122 (MQ=255)                                                                                                  aGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAgtgt                                  <  2:1196238/140‑1 (MQ=255)                                                                                                  aGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAgtgt                                  <  1:878459/140‑1 (MQ=255)                                                                                                  aGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAgtgt                                  <  1:1056981/140‑1 (MQ=255)                                                                                                                      aGCGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATg              >  2:380253/1‑140 (MQ=255)                                                                                                                        cGGGCCGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATAAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTa            <  2:199965/140‑1 (MQ=255)                                                                                                                            ccGTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTAAGCa        >  2:1057997/1‑140 (MQ=255)                                                                                                                              gTTGATCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTAAACAtt      >  2:1103633/1‑140 (MQ=255)                                                                                                                                  aTCAGGTGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTAAACATTGCgg  <  1:380253/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GCGCCGGTATCAACAAACTCACCTTCCAGCACAATTTCCAGCCGTGGAAAATCGACCTGATACGCAAGATCGGGCACCGGGCCGTTACTGCTGGCAAAGTAAATCTGGCGCAGGGACAGCGGGCCGTTGATCAGGCGGGAGAGCAGATAGCTGACGTCGTGATACATGTTATTCCCTTAGGTATGTAGACGGGAAAACAGTAACTGGCATTATTAAAAAAGGCCACCTGAAAAGTGTGGCCTGAGGGGAGTTCGATGTAAACATTGCGG  >  NC_000913/4148151‑4148419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑