Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,168,420	A→G	noncoding (49/76 nt)	gltT →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,168,420	0	A	G	100.0%	134.0 / NA	47	noncoding (49/76 nt)	gltT	tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/21);  total (26/21)

TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAA  >  NC_000913/4168341‑4168556                                                                                |                                                                                                                                         tatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCtt                                                                              >  1:895583/1‑140 (MQ=11)   tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                                            >  1:1114908/1‑140 (MQ=17)   tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                                            >  1:950562/1‑140 (MQ=17)   tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                                            >  2:133867/1‑140 (MQ=17)   tCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGAACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                                                                            >  1:867813/1‑140 (MQ=17)        cAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct                                                                       <  2:950562/140‑1 (MQ=18)        cAACGCGTGATAAGCAATTTTAGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct                                                                       <  2:1114908/140‑1 (MQ=18)          aCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCa                                                                     <  2:373228/140‑1 (MQ=18)          aCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGAACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCa                                                                     <  2:867813/140‑1 (MQ=18)           cgcgTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaa                                                                    >  1:1019426/1‑140 (MQ=17)             cgTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaaa                                                                  >  1:30340/1‑140 (MQ=17)             cgTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaaa                                                                  >  1:732430/1‑140 (MQ=17)              gTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAAc                                                                 <  2:553041/140‑1 (MQ=18)                 aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCa                                                              <  2:30340/140‑1 (MQ=25)                 aTAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCa                                                              >  2:570163/1‑140 (MQ=21)                         ttttCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCggg                                                      <  2:732430/140‑1 (MQ=255)                          tttCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGt                                                     <  2:1019426/140‑1 (MQ=255)                          tttCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGTGGAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCgggg                                                     >  1:81517/1‑139 (MQ=255)                           ttCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTTAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTg                                                    <  2:1144307/140‑1 (MQ=255)                           ttCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTg                                                    <  2:991691/140‑1 (MQ=255)                            tCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGa                                                   <  2:535041/140‑1 (MQ=255)                            tCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGa                                                   >  1:444508/1‑140 (MQ=255)                              gtgtCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATg                                                 <  1:169254/140‑1 (MQ=255)                                   cccTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTga                                            <  1:133867/140‑1 (MQ=38)                                   cccTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTga                                            <  1:266812/140‑1 (MQ=38)                                              gagGCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGctc                                 >  2:484102/1‑140 (MQ=255)                                                 gCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGAAGTTTGAGATATTTGCTCttt                              >  1:5421/1‑140 (MQ=255)                                                 gCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTGAGATATTTGCTCttt                              >  2:286293/1‑140 (MQ=255)                                                 gCCCAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTTGGGTGATGTTTGAGATATTTGCTCttt                              >  1:58104/1‑140 (MQ=255)                                                   ccAGGACACCGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTaa                            <  1:286293/140‑1 (MQ=255)                                                   ccAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTGGGAGTGAAAAGCGCCGAACCTAATATCTCAAAACTCATCTTTGGGGGATGGTTGAGATATTTGCTCTTTaa                            >  2:23206/1‑140 (MQ=11)                                                    cAGGACACCGCCCTTTCACGGCGGTAACGGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTaaa                           >  2:139908/1‑140 (MQ=255)                                                            cGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGa                   <  1:960783/140‑1 (MQ=37)                                                            cGCCCTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGa                   >  1:315213/1‑140 (MQ=37)                                                            cGCCCTTTCACGGCGGTAAAGGGGGTTCGAATCCCCTAGGGGGCGCCCCTTGCTGGTTTGTGAGGGAAAAACGCCGACCTTAATATTTCAAAACTCATCTTTCGGGGGTGTTTTAGATATTTTCTCTTTTAAAATCTggg                   >  2:925849/1‑139 (MQ=11)                                                            cGCCCTTTCACGGCGGTAAAGGGGGGTCGAATCCCCCAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATTTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGa                   >  1:777230/1‑140 (MQ=11)                                                            cGCCCTTTCACGGCGGTAAAGGGGGGTCCTATCCCCTAGGGGGCGCCCCTTGCTGGGTTGTGAGTGGAAGTCGCCGACCTTAATATCTCAAAACTCATTTTCGGGGGTTGTTTGAGAGATTTTCTTTTTTAAAATCTGGa                   >  2:288553/1‑140 (MQ=11)                                                             gCCCTTTCACGGCGGTAACGGGGGATCGAATCCCCTAGGGGCCACCACTTGCTGGTTTTGGAGGGCAAGTCGCCGGCCTTCATATCTCACAAATCATCTTCCGGAGCTGTTTGGGTTATTTTGTCTTTTTAAATCTGGaa                  >  2:1091073/1‑139 (MQ=2)                                                              cccTTTCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATc                 >  1:967668/1‑140 (MQ=37)                                                                 tttCACGGCGGTAACGGGTGTTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGGGAAAGGCGCCGACCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAg              >  2:481975/1‑140 (MQ=11)                                                                 tttCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTaa                            <  2:190114/126‑1 (MQ=38)                                                                 tttCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTaa                            >  1:190114/1‑126 (MQ=38)                                                                   tCACGGCGGTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCt            >  1:786883/1‑140 (MQ=37)                                                                      cggcggTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa         <  2:315213/140‑1 (MQ=37)                                                                      cggcggTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa         <  1:484102/140‑1 (MQ=37)                                                                      cggcggTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa         <  1:638751/140‑1 (MQ=37)                                                                      cggcggTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGAAGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa         <  2:5421/140‑1 (MQ=255)                                                                          ggTAACGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAAtt     >  1:534362/1‑140 (MQ=37)                                                                             aaCGGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGaa  >  2:978211/1‑140 (MQ=37)                                                                                |                                                                                                                                         TATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAA  >  NC_000913/4168341‑4168556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑