Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,246,520	C→T	intergenic (‑101/‑264)	malE ← / → malK	maltose ABC transporter periplasmic binding protein/maltose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,246,520	0	C	T	100.0%	261.6 / NA	73	intergenic (‑101/‑264)	malE/malK	maltose ABC transporter periplasmic binding protein/maltose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/34);  total (39/34)

AATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTT  >  NC_000913/4246385‑4246654                                                                                                                                        |                                                                                                                                       aaTGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCg                                                                                                                                    <  1:719421/140‑1 (MQ=255) aaTGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCg                                                                                                                                    <  1:998020/140‑1 (MQ=255)     cGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGc                                                                                                                                <  2:885469/140‑1 (MQ=255)     cGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGc                                                                                                                                >  1:569247/1‑140 (MQ=255)     cGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGc                                                                                                                                <  1:92216/140‑1 (MQ=255)       aGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCtt                                                                                                                              <  1:1042663/140‑1 (MQ=255)         gATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTtg                                                                                                                            >  2:864734/1‑140 (MQ=255)         gATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTtg                                                                                                                            >  2:1006002/1‑140 (MQ=255)             cGTGCACCTGTTTTTATTTTCATACTCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGGCTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTtgtgtg                                                                                                                        <  1:1006002/140‑1 (MQ=255)             cGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTtgtgtg                                                                                                                        <  2:1124685/140‑1 (MQ=255)             cGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTtgtgtg                                                                                                                        >  2:1178428/1‑140 (MQ=255)               tGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAt                                                                                                                      <  2:805949/140‑1 (MQ=255)                 cACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAtct                                                                                                                    <  2:98546/140‑1 (MQ=255)                 cACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAtct                                                                                                                    >  2:1219185/1‑140 (MQ=255)                 cACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAtct                                                                                                                    <  1:120841/140‑1 (MQ=255)                 cACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAtct                                                                                                                    <  1:1178428/140‑1 (MQ=255)                  aCCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGAtctc                                                                                                                   >  1:1096937/1‑140 (MQ=255)                       tttttATTTTCATAATCTATGGTCCTTGTCGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCACCCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTa                                                                                                              <  2:211130/140‑1 (MQ=255)                                tCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTgg                                                                                                     <  2:1196489/140‑1 (MQ=255)                                tCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTgg                                                                                                     <  2:734280/140‑1 (MQ=255)                                 cATAATCTATGGTCCTTGTTGGTGAAGCGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGc                                                                                                    >  1:1208444/1‑140 (MQ=255)                                  aTAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCg                                                                                                   <  1:393220/140‑1 (MQ=255)                                  aTAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTCTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCg                                                                                                   <  2:1092949/140‑1 (MQ=255)                                   tAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCgg                                                                                                  <  2:381677/140‑1 (MQ=255)                                   tAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCgg                                                                                                  <  2:53735/140‑1 (MQ=255)                                   tAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCgg                                                                                                  <  1:1219185/140‑1 (MQ=255)                                          tGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAAtgtg                                                                                           >  1:888899/1‑140 (MQ=255)                                          tGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAAtgtg                                                                                           <  1:350145/140‑1 (MQ=255)                                            gTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCgg                                                                                                  <  2:282750/131‑1 (MQ=255)                                            gTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCgg                                                                                                  >  1:282750/1‑131 (MQ=255)                                                ttgttgGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGGGATg                                                                                     >  2:1217336/1‑140 (MQ=255)                                                 tgttgGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATgc                                                                                    >  2:1100410/1‑140 (MQ=255)                                                            tGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAAt                                                                         >  2:73038/1‑140 (MQ=255)                                                             gCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATc                                                                        >  2:311262/1‑140 (MQ=255)                                                              cTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAAt                                                                         <  2:636857/138‑1 (MQ=255)                                                              cTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAAt                                                                         >  1:636857/1‑138 (MQ=255)                                                              cTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCg                                                                       >  1:441331/1‑140 (MQ=255)                                                              cTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCg                                                                       >  1:20396/1‑140 (MQ=255)                                                                         acCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGAttttt                                                            <  2:888899/140‑1 (MQ=255)                                                                          ccTAAACGGACTCTAGTTTCCTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTg                                                           >  1:115348/1‑140 (MQ=255)                                                                             aaaCGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTgcaa                                                        >  2:262929/1‑140 (MQ=255)                                                                                   aCTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACa                                                  >  1:916307/1‑140 (MQ=255)                                                                                   aCTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACa                                                  >  1:749899/1‑140 (MQ=255)                                                                                    ctctAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACAt                                                 <  1:73038/140‑1 (MQ=255)                                                                                    ctctAGTTTCCTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACAt                                                 <  2:115348/140‑1 (MQ=255)                                                                                     tctAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATc                                                >  1:702101/1‑140 (MQ=255)                                                                                     tctAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATc                                                >  1:592454/1‑140 (MQ=255)                                                                                        aGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACg                                             >  2:668608/1‑140 (MQ=255)                                                                                             cTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAAtt                                        <  2:749899/140‑1 (MQ=255)                                                                                              tttATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTc                                       <  2:441331/140‑1 (MQ=255)                                                                                               ttATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTcc                                      >  2:264391/1‑140 (MQ=255)                                                                                                 ataCGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTc                                       <  1:104947/137‑1 (MQ=255)                                                                                                 ataCGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTc                                       >  2:104947/1‑137 (MQ=255)                                                                                                   aCGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTAc                                  >  1:1156226/1‑140 (MQ=255)                                                                                                     ggCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACAt                                >  2:706719/1‑140 (MQ=255)                                                                                                     ggCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACAt                                <  1:262929/140‑1 (MQ=255)                                                                                                       cAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGa                              >  2:175188/1‑140 (MQ=255)                                                                                                       cAACCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGa                              >  1:315544/1‑140 (MQ=255)                                                                                                        aaCCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGAc                             >  1:1037543/1‑140 (MQ=255)                                                                                                        aaCCTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGAc                             <  2:214454/140‑1 (MQ=255)                                                                                                          ccTCTTTCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGTATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCt                           <  2:1096937/140‑1 (MQ=255)                                                                                                               ttCCATCCTCCTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGtt                      <  2:916307/140‑1 (MQ=255)                                                                                                                    tcctccTTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGtt                 <  2:592454/140‑1 (MQ=255)                                                                                                                     cctcctTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTc                >  1:812114/1‑140 (MQ=255)                                                                                                                        cctTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTcaca             >  2:1023670/1‑140 (MQ=255)                                                                                                                         ctTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAg            >  2:577144/1‑140 (MQ=255)                                                                                                                         ctTGCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAg            <  2:1156226/140‑1 (MQ=255)                                                                                                                            gCCCCTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAg         >  2:118153/1‑140 (MQ=255)                                                                                                                              cccTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGcc       >  1:1223375/1‑140 (MQ=255)                                                                                                                                cTACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCgt     >  1:549762/1‑140 (MQ=255)                                                                                                                                 tACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCgtg    >  2:111121/1‑140 (MQ=255)                                                                                                                                 tACGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCgtg    >  2:1179086/1‑140 (MQ=255)                                                                                                                                   cGCCCTACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGtt  >  1:216735/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       AATGCGAGGATGCGTGCACCTGTTTTTATTTTCATAATCTATGGTCCTTGTTGGTGAAGTGCTCGTGAAAACACCTAAACGGACTCTAGTTTCTTTATACGGCAACCTCTTTCCATCCTCCTTGCCCCTACGCCCCACCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGCAAGCAACATCACGAAATTCCTTACATGACCTCGGTTTAGTTCACAGAAGCCGTGTT  >  NC_000913/4246385‑4246654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑