Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,096	A→G	A286A (GCA→GCG)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,096	0	A	G	100.0%	212.7 / NA	58	A286A (GCA→GCG)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/29);  total (29/29)

TTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGC  >  NC_000913/371959‑372227                                                                                                                                          |                                                                                                                                    tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   <  1:291574/140‑1 (MQ=255) tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   <  1:790598/140‑1 (MQ=255) tttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   <  1:863371/140‑1 (MQ=255)  ttGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCgcgcgc                                                                                                                                  >  2:418176/1‑140 (MQ=255)       ccGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTa                                                                                                                             >  2:196354/1‑140 (MQ=255)           tggatgCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGAGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGa                                                                                                                         >  2:1065572/1‑140 (MQ=255)               tgCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   >  1:200488/1‑126 (MQ=255)               tgCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACT‑GTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGt                                                                                                                    >  2:568769/1‑140 (MQ=255)                 cGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   >  1:95175/1‑124 (MQ=255)                 cGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   <  2:95175/124‑1 (MQ=255)                 cGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTcgcgcg                                                                                                                                   >  2:337219/1‑124 (MQ=255)                 cGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTc                                                                                                                   >  1:1208926/1‑140 (MQ=255)                  gggTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCa                                                                                                                  <  1:418176/140‑1 (MQ=255)                    gtctgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAAGATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATg                                                                                                                >  1:24009/1‑140 (MQ=255)                      ctgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTT‑AATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACg                                                                                                             <  2:710851/140‑1 (MQ=255)                       tgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACg                                                                                                             >  2:46443/1‑140 (MQ=255)                       tgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACg                                                                                                             <  1:845550/140‑1 (MQ=255)                       tgcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACg                                                                                                             <  1:890402/140‑1 (MQ=255)                        gcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGa                                                                                                            >  1:675551/1‑140 (MQ=255)                        gcgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGa                                                                                                            >  2:863577/1‑140 (MQ=255)                         cgtctgcTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGaa                                                                                                           <  1:863577/140‑1 (MQ=255)                              gctgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCACGCGCCCTTAAGGAATGGTCATGACGAAGCATa                                                                                                      >  1:341562/1‑140 (MQ=255)                                tgTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATAct                                                                                                    <  2:1090108/140‑1 (MQ=255)                                  tCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACAAGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATActct                                                                                                  >  1:1069911/1‑140 (MQ=255)                                   ccGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCtt                                                                                                 >  2:727201/1‑140 (MQ=255)                                   ccGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCACGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCtt                                                                                                 <  2:341562/140‑1 (MQ=255)                                          tGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAAc                                                                                          >  1:917926/1‑140 (MQ=255)                                               gTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACT‑GTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTggcgg                                                                                    <  1:568769/140‑1 (MQ=255)                                                ttCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTggcgg                                                                                    <  2:1069911/140‑1 (MQ=255)                                                          cATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGAt                                                                               >  1:132302/1‑135 (MQ=255)                                                          cATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGAt                                                                               <  2:132302/135‑1 (MQ=255)                                                          cATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcg                                                                          >  1:769647/1‑140 (MQ=255)                                                            tatCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgcc                                                                        >  2:949803/1‑140 (MQ=255)                                                             atCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       <  2:769647/140‑1 (MQ=255)                                                             atCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       <  1:1065572/140‑1 (MQ=255)                                                             atCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       <  2:983003/140‑1 (MQ=255)                                                             atCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       <  1:949803/140‑1 (MQ=255)                                                               cTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccgcg                                                                     >  1:79135/1‑140 (MQ=255)                                                               cTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       <  1:915450/138‑1 (MQ=255)                                                               cTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccg                                                                       >  2:915450/1‑138 (MQ=255)                                                                  ccGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCTCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccgcgccg                                                                  <  2:79135/140‑1 (MQ=255)                                                                  ccGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccgcgccg                                                                  <  1:727201/140‑1 (MQ=255)                                                                  ccGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccgcgccg                                                                  <  1:762973/140‑1 (MQ=255)                                                                  ccGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTAcgccgcgccg                                                                  >  1:359320/1‑140 (MQ=255)                                                                    gcgACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCa                                                                >  2:946559/1‑140 (MQ=255)                                                                    gcgACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCa                                                                >  2:1067052/1‑140 (MQ=255)                                                                               cACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGa                                                     <  2:675551/140‑1 (MQ=255)                                                                               cACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGCTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGa                                                     <  2:1138295/140‑1 (MQ=255)                                                                                aCTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGaa                                                    >  1:868476/1‑140 (MQ=255)                                                                                  tGGGCGCAGTGGGAAGATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGc                                                  <  2:24009/140‑1 (MQ=255)                                                                                  tGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGc                                                  >  2:725327/1‑140 (MQ=255)                                                                                     gcgcAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGAt                                               >  1:1124485/1‑140 (MQ=255)                                                                                         aGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCa                                           <  1:946559/140‑1 (MQ=255)                                                                                         aGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCa                                           <  2:917926/140‑1 (MQ=255)                                                                                            gggAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCg                                        >  1:1093349/1‑140 (MQ=255)                                                                                               aaCATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTATTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTg                                     <  2:1093349/140‑1 (MQ=255)                                                                                                     ccGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGAt                               >  2:523821/1‑140 (MQ=255)                                                                                                     ccGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGAt                               >  2:1210236/1‑140 (MQ=255)                                                                                                          gCTTTCAATCAACTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGAt                          <  2:1124485/140‑1 (MQ=255)                                                                                                              tcaatcaaCTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGt                      <  2:868476/140‑1 (MQ=255)                                                                                                                      cTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGt                      <  1:281561/132‑1 (MQ=255)                                                                                                                      cTGGTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGt                      >  2:281561/1‑132 (MQ=255)                                                                                                                        ggTGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACg            >  1:150530/1‑140 (MQ=255)                                                                                                                          tGCTGAATTTCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCt          >  1:59990/1‑140 (MQ=255)                                                                                                                                  ttCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGc  <  2:441048/140‑1 (MQ=255)                                                                                                                                  ttCCTCGCGCGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGc  <  2:359320/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    TTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCCGGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGACGCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAAGGAATGGTCATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGCGAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGC  >  NC_000913/371959‑372227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑