Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,861	T→C	intergenic (‑88/+30)	dcuA ← / ← aspA	C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,861	0	T	C	100.0%	286.1 / NA	78	intergenic (‑88/+30)	dcuA/aspA	C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/41);  total (37/41)

AAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGT  >  NC_000913/4366730‑4366996                                                                                                                                    |                                                                                                                                        aagaagATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCttttt                                                                                                                                 >  2:186144/1‑140 (MQ=255)  agaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCtttttt                                                                                                                                <  2:734593/140‑1 (MQ=255)    aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              <  2:193923/140‑1 (MQ=255)    aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              <  1:1126130/140‑1 (MQ=255)    aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              <  2:78435/140‑1 (MQ=255)    aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              <  2:603663/140‑1 (MQ=255)         cGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              <  1:805892/135‑1 (MQ=255)         cGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTAt                                                                                                                              >  2:805892/1‑135 (MQ=255)         cGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGta                                                                                                                         >  1:861434/1‑140 (MQ=255)         cGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCATTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGta                                                                                                                         <  1:1041073/140‑1 (MQ=255)            cAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGtacta                                                                                                                      <  2:981333/140‑1 (MQ=255)            cAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGtacta                                                                                                                      <  1:1012578/140‑1 (MQ=255)            cAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGtacta                                                                                                                      <  2:314064/140‑1 (MQ=255)             aGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGACACGTCATCTGACGCGCCTTTTTTATTTGtactac                                                                                                                     >  1:1151543/1‑140 (MQ=255)              gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCttt                                                                                                                                   >  1:258494/1‑125 (MQ=255)              gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCttt                                                                                                                                   <  2:258494/125‑1 (MQ=255)              gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTAcc                                                                                                                    <  1:186144/140‑1 (MQ=255)              gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTAcc                                                                                                                    >  2:949709/1‑140 (MQ=255)              gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTAcc                                                                                                                    >  2:1093368/1‑140 (MQ=255)               cAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTAccc                                                                                                                   <  1:398755/140‑1 (MQ=255)               cAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTAccc                                                                                                                   <  1:1168112/140‑1 (MQ=255)               cAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCACCTTTTTTATTTGTACTAccc                                                                                                                   <  2:448831/140‑1 (MQ=255)                aaaaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCtttt                                                                                                                                  >  1:428573/1‑124 (MQ=255)                aaaaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCtttt                                                                                                                                  <  2:428573/124‑1 (MQ=255)                         atgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGACAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTa                                                                                                         >  2:1067131/1‑140 (MQ=255)                         atgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTa                                                                                                         >  2:528466/1‑140 (MQ=255)                               tCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTc                                                                                                   >  2:469517/1‑140 (MQ=255)                                   cAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCtt                                                                                               >  2:706752/1‑140 (MQ=255)                                      cTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtca                                                                                            <  1:766919/140‑1 (MQ=255)                                      cTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtca                                                                                            >  2:974390/1‑140 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          >  2:100868/1‑140 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          >  2:1035757/1‑140 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          >  2:592193/1‑140 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          >  2:897382/1‑140 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          <  1:141942/140‑1 (MQ=255)                                        aGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc                                                                                          >  1:354415/1‑140 (MQ=255)                                               tAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAg                                                                                   >  1:449734/1‑140 (MQ=255)                                                   cttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGtt                                                                               <  1:739202/140‑1 (MQ=255)                                                     tccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGtttt                                                                             <  1:592193/140‑1 (MQ=255)                                                      ccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTg                                                                            <  1:897382/140‑1 (MQ=255)                                                      ccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTg                                                                            >  2:59411/1‑140 (MQ=255)                                                      ccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTg                                                                            >  2:480300/1‑140 (MQ=255)                                                      ccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTg                                                                            >  2:257525/1‑140 (MQ=255)                                                       cttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGc                                                                           >  1:1193254/1‑140 (MQ=255)                                                         tGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtt                                                                         >  2:1153167/1‑140 (MQ=255)                                                               ttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAg                                                                   <  2:494880/140‑1 (MQ=255)                                                                 aaCAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGcc                                                                 <  1:706752/140‑1 (MQ=255)                                                                   cAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCgg                                                               <  1:1093368/140‑1 (MQ=255)                                                                     aGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                             <  1:100868/140‑1 (MQ=255)                                                                     aGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                             >  2:79405/1‑140 (MQ=255)                                                                     aGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                             <  2:1097465/140‑1 (MQ=255)                                                                      gTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTg                                                            >  2:741314/1‑140 (MQ=255)                                                                          atatTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATc                                                        <  1:59411/140‑1 (MQ=255)                                                                                gATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTc                                                  >  1:1000836/1‑140 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACATGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             >  2:704868/1‑140 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATc                                                        <  1:670613/129‑1 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATc                                                        >  2:670613/1‑129 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAg                                                      <  2:807630/131‑1 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAg                                                      >  1:807630/1‑131 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             <  1:949709/140‑1 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             <  2:861434/140‑1 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             >  2:793553/1‑140 (MQ=255)                                                                                     ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             <  1:1213247/140‑1 (MQ=255)                                                                                                  aCTGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCg                                >  2:1006149/1‑140 (MQ=255)                                                                                                    tGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt                                               >  1:920796/1‑123 (MQ=255)                                                                                                    tGCTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt                                               <  2:920796/123‑1 (MQ=255)                                                                                                      cTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             >  1:243610/1‑123 (MQ=255)                                                                                                      cTCACAAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTAc                                             <  2:243610/123‑1 (MQ=255)                                                                                                         acaAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTGCCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGt                               <  1:1053507/134‑1 (MQ=255)                                                                                                         acaAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGt                               >  2:1053507/1‑134 (MQ=255)                                                                                                         acaAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGtt                         >  2:502968/1‑140 (MQ=255)                                                                                                         acaAGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGtt                         <  1:1153167/140‑1 (MQ=255)                                                                                                            aGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCg                      <  1:958844/140‑1 (MQ=255)                                                                                                            aGAAAAAAGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCg                      <  2:449734/140‑1 (MQ=255)                                                                                                              aaaaaaGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCt                    <  1:79405/140‑1 (MQ=255)                                                                                                                aaaaGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc                  <  1:502968/140‑1 (MQ=255)                                                                                                                   aGGCACGTCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGt               >  1:1064273/1‑140 (MQ=255)                                                                                                                          tCATCTGACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGa        <  2:354415/140‑1 (MQ=255)                                                                                                                                gACGCGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGt  <  1:704868/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        AAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGT  >  NC_000913/4366730‑4366996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑