Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,626	C→T	intergenic (‑1/‑354)	ulaG ← / → ulaA	L‑ascorbate‑6‑phosphate lactonase/L‑ascorbate specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,626	0	C	T	100.0%	258.8 / NA	72	intergenic (‑1/‑354)	ulaG/ulaA	L‑ascorbate‑6‑phosphate lactonase/L‑ascorbate specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/34);  total (38/34)

TGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTA  >  NC_000913/4419490‑4419759                                                                                                                                         |                                                                                                                                      tGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGcc                                                                                                                                    >  2:600020/1‑140 (MQ=255)    agccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 <  1:994681/140‑1 (MQ=255)    agccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 >  2:1004349/1‑140 (MQ=255)    agccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 <  2:46907/140‑1 (MQ=255)     gccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAttt                                                                                                                                <  2:985608/140‑1 (MQ=255)     gccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAttt                                                                                                                                <  2:43511/140‑1 (MQ=255)     gccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAttt                                                                                                                                >  2:668702/1‑140 (MQ=255)      ccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTa                                                                                                                               >  2:973071/1‑140 (MQ=255)      ccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTa                                                                                                                               >  2:881255/1‑140 (MQ=255)      ccaagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTa                                                                                                                               >  2:493426/1‑140 (MQ=255)        aagccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTAcc                                                                                                                             <  1:1004349/140‑1 (MQ=255)          gccaCCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCtt                                                                                                                           >  1:903719/1‑140 (MQ=255)           ccaccaCATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTc                                                                                                                          >  1:39433/1‑140 (MQ=255)           ccaccaCATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTc                                                                                                                          >  1:1061320/1‑140 (MQ=255)               cacaTCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGa                                                                                                                      <  1:493426/140‑1 (MQ=255)               cacaTCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGa                                                                                                                      <  1:321325/140‑1 (MQ=255)                acaTCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGAt                                                                                                                     >  1:578916/1‑140 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 <  2:829166/124‑1 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 <  2:1084401/124‑1 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 >  1:1084401/1‑124 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCAtt                                                                                                                                 >  1:829166/1‑124 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGtt                                                                                                                 >  2:574711/1‑140 (MQ=255)                    cGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGtt                                                                                                                 >  1:121523/1‑140 (MQ=255)                      cAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCa                                                                                                               >  2:959136/1‑140 (MQ=255)                         aTGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAAtt                                                                                                            >  1:1005244/1‑140 (MQ=255)                          tgtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTc                                                                                                           <  2:1061320/140‑1 (MQ=255)                           gtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCg                                                                                                          <  2:578916/140‑1 (MQ=255)                            tgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATg                                                                                                                       <  2:614522/126‑1 (MQ=255)                            tgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATg                                                                                                                       >  1:614522/1‑126 (MQ=255)                            tgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGa                                                                                                         <  1:959136/140‑1 (MQ=255)                              ccAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAAt                                                                                                       <  1:668702/140‑1 (MQ=255)                              ccAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAAt                                                                                                       >  1:568687/1‑140 (MQ=255)                                aGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCa                                                                                                     <  1:1139022/140‑1 (MQ=255)                                 ggAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGGTTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCaa                                                                                                    <  2:445876/140‑1 (MQ=255)                                 ggAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCaa                                                                                                    >  2:131426/1‑140 (MQ=255)                                  gAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAat                                                                                                   <  2:1005244/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  2:39433/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  2:730052/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  2:568687/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  2:1017541/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  1:509176/140‑1 (MQ=255)                                         cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGAt                                                                                            <  2:608972/140‑1 (MQ=255)                                              cTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGtt                                                                                       <  2:121523/140‑1 (MQ=255)                                                  ttCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGa                                                                                   >  1:298325/1‑140 (MQ=255)                                                        ttcttcATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATc                                                                             <  2:903719/140‑1 (MQ=255)                                                         tcttcATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATcc                                                                            <  1:600020/140‑1 (MQ=255)                                                            tcattcaACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGa                                                                         >  2:947295/1‑140 (MQ=255)                                                                    ccAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTAttt                                                                 >  1:868549/1‑140 (MQ=255)                                                                       gCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTAtttttt                                                              <  1:343021/140‑1 (MQ=255)                                                                        cTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTc                                                             >  1:797663/1‑140 (MQ=255)                                                                           ccccACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAgg                                                          <  1:49394/140‑1 (MQ=255)                                                                                     ggAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCTTTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGa                                                >  1:908654/1‑140 (MQ=255)                                                                                     ggAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAgg                                                          >  1:1082364/1‑130 (MQ=255)                                                                                     ggAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAgg                                                          <  2:1082364/130‑1 (MQ=255)                                                                                     ggAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGa                                                >  2:114599/1‑140 (MQ=255)                                                                                     ggAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGa                                                >  1:645140/1‑140 (MQ=255)                                                                                          gTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCAtt                                           >  1:553348/1‑140 (MQ=255)                                                                                           tGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCAttt                                          >  1:256173/1‑140 (MQ=255)                                                                                             cTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCAttttt                                        >  2:1095075/1‑140 (MQ=255)                                                                                               cAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTg                                      >  1:59366/1‑140 (MQ=255)                                                                                                            tCACGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAgg                         >  1:795746/1‑140 (MQ=255)                                                                                                              aCGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGa                       >  1:589266/1‑140 (MQ=255)                                                                                                              aCGGGTGATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGa                       <  1:114599/140‑1 (MQ=255)                                                                                                                    gATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAgttgt                 <  2:589266/140‑1 (MQ=255)                                                                                                                    gATACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAgttgt                 <  1:862121/140‑1 (MQ=255)                                                                                                                     aTACTTTTCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAgttgtt                >  2:1044213/1‑140 (MQ=255)                                                                                                                           ttCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGAt          >  1:614084/1‑140 (MQ=255)                                                                                                                            tCACTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGAtt         >  1:982096/1‑140 (MQ=255)                                                                                                                              aCTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGAtttt       <  1:973071/140‑1 (MQ=255)                                                                                                                              aCTTTACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGAtttt       <  2:797663/140‑1 (MQ=255)                                                                                                                                  tACTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGt   >  2:379529/1‑140 (MQ=255)                                                                                                                                   aCTCATTGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTa  >  1:749015/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCATGATAGTTCAATTCGAATCAATATGTGATTGGTTTTGATTAATCCTGACACTATTTTTTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTA  >  NC_000913/4419490‑4419759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑