Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,918	A→G	G216G (GGT→GGC)	cpdB ←	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,918	0	A	G	100.0%	301.2 / NA	81	G216G (GGT→GGC)	cpdB	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/46);  total (35/46)

AACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCC  >  NC_000913/4435783‑4436043                                                                                                                                        |                                                                                                                              aaCGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCtt                                                                                                                           >  1:586301/1‑140 (MQ=255)      cGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTcg                                                                                                                      <  1:1061274/140‑1 (MQ=255)      cGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTcg                                                                                                                      <  2:1116348/140‑1 (MQ=255)      cGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTcg                                                                                                                      <  1:785642/140‑1 (MQ=255)       gTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTcgc                                                                                                                     >  1:347329/1‑140 (MQ=255)              tGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTc                                                                                                              <  1:439107/140‑1 (MQ=255)                 ccAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAgg                                                                                                           <  1:352943/140‑1 (MQ=255)                 ccAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAgg                                                                                                           <  1:633267/140‑1 (MQ=255)                   aaaCATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCa                                                                                                         <  2:586301/140‑1 (MQ=255)                    aaCATAATGGCGTTAACGCCCGGAATTTCACTGAGTTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAgg                                                                                                           >  2:196747/1‑137 (MQ=255)                    aaCATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAgg                                                                                                           <  1:196747/137‑1 (MQ=255)                          aTGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTAttt                                                                                                  >  2:302129/1‑140 (MQ=255)                           tGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTg                                                                                                 >  2:523150/1‑140 (MQ=255)                           tGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTg                                                                                                 >  2:300634/1‑140 (MQ=255)                              cGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTgcgc                                                                                              <  1:300634/140‑1 (MQ=255)                               gTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCa                                                                                             <  1:1001044/140‑1 (MQ=255)                                ttAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTc                                                                                                              >  2:113076/1‑122 (MQ=255)                                ttAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTc                                                                                                              <  1:113076/122‑1 (MQ=255)                                ttAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCAc                                                                                            >  2:302841/1‑140 (MQ=255)                                ttAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCAc                                                                                            >  1:352784/1‑140 (MQ=255)                                ttAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCAc                                                                                            <  2:875743/140‑1 (MQ=255)                                   aCGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGt                                                                                         <  1:594828/140‑1 (MQ=255)                                   aCGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGt                                                                                         <  2:469531/140‑1 (MQ=255)                                   aCGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGt                                                                                         <  1:423249/140‑1 (MQ=255)                                        cGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTGGCGCATTTCAGGCACGTATTTGCGCACGGTTTCgg                                                                                    <  1:587055/140‑1 (MQ=255)                                        cGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCgg                                                                                    <  2:422913/140‑1 (MQ=255)                                        cGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCgg                                                                                    >  1:422913/1‑140 (MQ=255)                                          gAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTa                                                                                  <  1:588985/140‑1 (MQ=255)                                                cACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCa                                                                            >  2:1048567/1‑140 (MQ=255)                                                    gAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCa                                                                        >  1:837295/1‑140 (MQ=255)                                                     aGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCAc                                                                       <  2:1165060/140‑1 (MQ=255)                                                     aGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCAc                                                                       >  1:1165060/1‑140 (MQ=255)                                                             aaCTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACtt                                                               <  2:1022697/140‑1 (MQ=255)                                                               cTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTc                                                             >  2:350020/1‑140 (MQ=255)                                                                 gAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTccc                                                           >  2:617414/1‑140 (MQ=255)                                                                 gAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTccc                                                           >  2:896023/1‑140 (MQ=255)                                                                  aGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCg                                                          <  2:347329/140‑1 (MQ=255)                                                                  aGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCg                                                          <  1:896023/140‑1 (MQ=255)                                                                         cGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAt                                                   <  2:766312/140‑1 (MQ=255)                                                                              tCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCt                                              >  2:3324/1‑140 (MQ=255)                                                                              tCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCt                                              <  1:1127484/140‑1 (MQ=255)                                                                                   ttATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATc                                         <  2:837295/140‑1 (MQ=255)                                                                                   ttATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATc                                         <  1:523150/140‑1 (MQ=255)                                                                                          gATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGccc                                  >  2:346426/1‑140 (MQ=255)                                                                                          gATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGccc                                  >  2:730892/1‑140 (MQ=255)                                                                                           aTCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 >  1:118093/1‑140 (MQ=255)                                                                                           aTCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 >  1:1152682/1‑140 (MQ=255)                                                                                           aTCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 >  1:847206/1‑140 (MQ=255)                                                                                           aTCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 >  1:575334/1‑140 (MQ=255)                                                                                            tCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 <  1:1038406/139‑1 (MQ=255)                                                                                            tCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCa                                 >  2:1038406/1‑139 (MQ=255)                                                                                            tCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCAt                                >  2:139423/1‑140 (MQ=255)                                                                                               gCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGAt                             <  1:139423/140‑1 (MQ=255)                                                                                               gCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGAt                             <  2:352784/140‑1 (MQ=255)                                                                                                cAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGAtt                            >  1:755463/1‑140 (MQ=255)                                                                                                   aTAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTtgt                         >  1:417658/1‑140 (MQ=255)                                                                                                   aTAGCCCGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTtgt                         <  2:575334/140‑1 (MQ=255)                                                                                                       cccGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGAt                             >  2:1188374/1‑132 (MQ=255)                                                                                                       cccGGAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGAt                             <  1:1188374/132‑1 (MQ=255)                                                                                                          ggAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCa                  <  1:730892/140‑1 (MQ=255)                                                                                                           gAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  2:118093/140‑1 (MQ=255)                                                                                                           gAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  1:346426/140‑1 (MQ=255)                                                                                                           gAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  1:617414/140‑1 (MQ=255)                                                                                                           gAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  1:78587/140‑1 (MQ=255)                                                                                                           gAATGTGCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  1:526516/140‑1 (MQ=255)                                                                                                              tgtgCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgac              <  1:350020/140‑1 (MQ=255)                                                                                                              tgtgCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgac              <  1:453822/140‑1 (MQ=255)                                                                                                               gtgCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCTCCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgacg             <  2:847206/140‑1 (MQ=255)                                                                                                               gtgCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTATCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgacg             >  1:392344/1‑140 (MQ=255)                                                                                                                tgCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGc            >  2:5775/1‑140 (MQ=255)                                                                                                                 gCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgac              <  1:1098126/137‑1 (MQ=255)                                                                                                                 gCCAGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCacgac              >  2:1098126/1‑137 (MQ=255)                                                                                                                    aGAACGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAat        <  1:643475/140‑1 (MQ=255)                                                                                                                      aaCGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACTGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAatat      >  1:679566/1‑140 (MQ=255)                                                                                                                      aaCGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGc            <  1:61056/134‑1 (MQ=255)                                                                                                                      aaCGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGc            >  2:61056/1‑134 (MQ=255)                                                                                                                      aaCGACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAatat      >  1:770662/1‑140 (MQ=255)                                                                                                                         gACAACAACATCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGc   <  1:3324/140‑1 (MQ=255)                                                                                                                          acaacaacaTCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 <  2:329619/125‑1 (MQ=255)                                                                                                                          acaacaacaTCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCac                 >  1:329619/1‑125 (MQ=255)                                                                                                                          acaacaacaTCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGcc  >  1:605664/1‑140 (MQ=255)                                                                                                                          acaacaacaTCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGcc  <  2:605664/140‑1 (MQ=255)                                                                                                                          acaacaacaTCGGCGCCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGcc  >  1:687491/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                              AACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCC  >  NC_000913/4435783‑4436043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑