Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,569,605	A→G	pseudogene (608/921 nt)	rpnD →	truncated recombination‑promoting nuclease‑like protein RpnD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,569,605	0	A	G	100.0%	272.0 / NA	74	pseudogene (608/921 nt)	rpnD	truncated recombination‑promoting nuclease‑like protein RpnD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/29);  total (45/29)

TGGATGTTACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGAGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGATTGC  >  NC_000913/4569470‑4569740                                                                                                                                        |                                                                                                                                        tggATGTTACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGgtgt                                                                                                                                     >  2:79665/1‑140 (MQ=255)   gATGTTACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGc                                                                                                                                   <  2:650284/140‑1 (MQ=255)      gTTACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTaa                                                                                                                                <  1:315708/140‑1 (MQ=255)        tACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAAt                                                                                                                               <  2:208550/139‑1 (MQ=255)        tACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAAt                                                                                                                               >  1:208550/1‑139 (MQ=255)          cTGTCGTGCCAGACGTCGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGAc                                                                                                                            <  2:1119709/140‑1 (MQ=255)          cTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGAc                                                                                                                            <  2:251470/140‑1 (MQ=255)                tGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCa                                                                                                                       <  2:961694/139‑1 (MQ=255)                tGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCa                                                                                                                       >  1:961694/1‑139 (MQ=255)                  ccAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGAt                                                                                                                    >  2:1066613/1‑140 (MQ=255)                       cgacgaGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTg                                                                                                               >  1:91744/1‑140 (MQ=255)                          cgaTATTGTGCAGCATCGCAGAGTTGCTCTGTTAGAGTTGGTGCAAAAACATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTTGTAGTATTACTGGTTACAGGGTGTGTTTATGACAGCCAGATATCTgcgc                                                                                                            <  2:878369/140‑1 (MQ=255)                          cgaGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTgcgc                                                                                                            <  2:449402/140‑1 (MQ=255)                                  tgCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAAt                                                                                                    >  2:585193/1‑140 (MQ=255)                                    cagcaTCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTg                                                                                                          >  1:747754/1‑132 (MQ=255)                                    cagcaTCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTg                                                                                                          <  2:747754/132‑1 (MQ=255)                                    cagcaTCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTg                                                                                                          <  2:1095511/132‑1 (MQ=255)                                    cagcaTCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTg                                                                                                          >  1:1095511/1‑132 (MQ=255)                                          cGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACAtttt                                                                                            >  1:486873/1‑140 (MQ=255)                                            cAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTac                                                                                          >  2:914972/1‑140 (MQ=255)                                               agTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTA‑TACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTactgac                                                                                      <  1:388269/140‑1 (MQ=255)                                                gTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTactgac                                                                                      <  1:79665/140‑1 (MQ=255)                                                 tCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTactgact                                                                                     >  1:240832/1‑140 (MQ=255)                                                    cccTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGc                                                                                  >  1:263804/1‑140 (MQ=255)                                                    cccTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGc                                                                                  >  1:434117/1‑140 (MQ=255)                                                    cccTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGc                                                                                  >  2:778961/1‑140 (MQ=255)                                                                 gATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGtt                                                                     >  2:562658/1‑140 (MQ=255)                                                                   tCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGtttt                                                                   >  1:140560/1‑140 (MQ=255)                                                                    ccAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTa                                                                  <  2:434117/140‑1 (MQ=255)                                                                      aaaaGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAAt                                                                <  1:585193/140‑1 (MQ=255)                                                                              atTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTAt                                                        >  2:225090/1‑140 (MQ=255)                                                                                 cGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAg                                                     >  1:1143092/1‑140 (MQ=255)                                                                                    cAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAAt                                                                >  2:174355/1‑126 (MQ=255)                                                                                    cAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAAt                                                                <  1:174355/126‑1 (MQ=255)                                                                                    cAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGa                                                  >  1:226048/1‑140 (MQ=255)                                                                                     aGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGaa                                                 >  1:456538/1‑140 (MQ=255)                                                                                     aGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGaa                                                 >  1:452703/1‑140 (MQ=255)                                                                                          gATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTAc                                            >  1:854273/1‑140 (MQ=255)                                                                                                aTGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCg                                      >  2:709485/1‑140 (MQ=255)                                                                                                  ggggCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    <  1:425365/140‑1 (MQ=255)                                                                                                  ggggCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    <  1:709485/140‑1 (MQ=255)                                                                                                    ggCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATg                                  >  1:89698/1‑140 (MQ=255)                                                                                                    ggCTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATg                                  >  2:625243/1‑140 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    <  2:1210385/136‑1 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    >  1:1210385/1‑136 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                <  2:1087002/140‑1 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                >  1:467998/1‑140 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                >  2:12235/1‑140 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                >  1:478039/1‑140 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                >  1:1087002/1‑140 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                <  2:226048/140‑1 (MQ=255)                                                                                                      cTTATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGcc                                <  1:1066613/140‑1 (MQ=255)                                                                                                        tATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGTTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCcac                              >  1:1059797/1‑140 (MQ=255)                                                                                                        tATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCcac                              <  2:467998/140‑1 (MQ=255)                                                                                                        tATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCcac                              >  2:939983/1‑140 (MQ=255)                                                                                                        tATCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCcac                              >  1:849885/1‑140 (MQ=255)                                                                                                          tCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    >  2:623314/1‑132 (MQ=255)                                                                                                          tCGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGaa                                    <  1:623314/132‑1 (MQ=255)                                                                                                           cGATCAACTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCacaac                           >  1:513464/1‑140 (MQ=255)                                                                                                                 aCTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAggg                     >  2:958331/1‑140 (MQ=255)                                                                                                                  cTGGTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGa                    <  2:854273/140‑1 (MQ=255)                                                                                                                    ggTAGTATTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGc                  <  1:12235/140‑1 (MQ=255)                                                                                                                     gtagtaTTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCg                 >  2:973263/1‑140 (MQ=255)                                                                                                                      tagtaTTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGa                <  1:625243/140‑1 (MQ=255)                                                                                                                         taTTACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGaata             <  1:973263/140‑1 (MQ=255)                                                                                                                            tACTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATg          >  1:1140032/1‑140 (MQ=255)                                                                                                                              cTGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGAc        <  2:456538/140‑1 (MQ=255)                                                                                                                               tGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACg       >  1:789564/1‑140 (MQ=255)                                                                                                                               tGGTTACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACg       >  1:1007021/1‑140 (MQ=255)                                                                                                                                  ttACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGAtt    >  2:905262/1‑140 (MQ=255)                                                                                                                                   tACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGATTg   >  2:210701/1‑140 (MQ=255)                                                                                                                                   tACAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAAAGACGATTg   >  1:541849/1‑140 (MQ=255)                                                                                                                                   tACAGGGTGTGCTAATAACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGGGCGTTTTAATGAGTTTATCAATGAGCTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGATTg   >  2:539115/1‑140 (MQ=255)                                                                                                                                    aCAGGGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGATTGc  <  2:140560/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGGATGTTACTGTCGTGCCAGACGACGAGATTGTGCAGCATCGCAGAGTCGCCCTGTTGGAGTTGATCCAAAAGCATATTCGCCAGCGCGATCTGATGGGGCTTATCGATCAACTGGTAGTATTACTGGTTACAGAGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACATTTTACTGACTGGCGATGAAGCGCGTTTTAATGAGTTTATCAGTGAACTTACCCGTCGAATGCCACAACACAGGGAGCGAATAATGACGATTGC  >  NC_000913/4569470‑4569740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑