Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,714	T→C	D198G (GAC→GGC)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,714	0	T	C	100.0%	189.7 / NA	52	D198G (GAC→GGC)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

GCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGG  >  NC_000913/4639585‑4639848                                                                                                                                  |                                                                                                                                       gCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGttt                                                                                                                              <  1:990419/140‑1 (MQ=255) gCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGttt                                                                                                                              <  2:797035/140‑1 (MQ=255)    gATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAg                                                                                                                           >  1:973089/1‑140 (MQ=255)      ttAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCt                                                                                                                         >  2:695710/1‑140 (MQ=255)               cAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCgg                                                                                                                >  2:423598/1‑140 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCgg                                                                                                                >  2:548033/1‑132 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCgg                                                                                                                <  1:548033/132‑1 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCAtt                                                                                                           <  2:914908/137‑1 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCAtt                                                                                                           >  1:914908/1‑137 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTttc                                                                                                        >  2:62561/1‑140 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTttc                                                                                                        >  2:844067/1‑140 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTttc                                                                                                        <  1:844067/140‑1 (MQ=255)                       cGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGGGCGGTTTCAGCTCACGGCCGGTCATTttc                                                                                                        >  2:518894/1‑140 (MQ=255)                            aaGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcag                                                                                                   <  1:1049106/140‑1 (MQ=255)                              gCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGGCATTTTCTTcagca                                                                                                 >  2:160563/1‑140 (MQ=255)                                gATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt                                                                                               >  1:664883/1‑140 (MQ=255)                                 aTAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCCCGTCTACAGTACGGCCGCGCGGTTTCCGCTCACCGCCGGTCATTTTCTTCCGCAAtt                                                                                              >  2:17464/1‑140 (MQ=255)                                 aTAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGtt                                                                                              >  2:763761/1‑140 (MQ=255)                                      cTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCa                                                                                         <  2:1038300/140‑1 (MQ=255)                                      cTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCa                                                                                         <  1:956979/140‑1 (MQ=255)                                      cTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCa                                                                                         <  1:199385/140‑1 (MQ=255)                                         cACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcag                                                                                                   <  2:211126/127‑1 (MQ=255)                                         cACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcag                                                                                                   >  1:211126/1‑127 (MQ=255)                                              tGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTg                                                                                 >  1:102868/1‑140 (MQ=255)                                                aaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCa                                                                                            >  2:708705/1‑127 (MQ=255)                                                aaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCa                                                                                            <  1:708705/127‑1 (MQ=255)                                                aaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGaa                                                                               >  2:601486/1‑140 (MQ=255)                                                aaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGaa                                                                               >  1:850851/1‑140 (MQ=255)                                                aaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATAGTTACGGCTACAGTACGGCCGCGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGaa                                                                               >  1:810211/1‑140 (MQ=255)                                                     tggCTATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTg                                                                          >  2:290800/1‑140 (MQ=255)                                                      ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGc                                                                         >  2:730523/1‑140 (MQ=255)                                                       gCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGcc                                                                        <  1:423598/140‑1 (MQ=255)                                                          atgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTgg                                                                     >  1:1078727/1‑140 (MQ=255)                                                          atgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTgg                                                                     <  1:730523/140‑1 (MQ=255)                                                               ttCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGtttt                                                                <  1:695710/140‑1 (MQ=255)                                                                  cggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACCGAATTTTGCCTGGGTTTTcac                                                             <  2:869952/140‑1 (MQ=255)                                                                     cgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTCTGCCTGGGTTTTCACAGa                                                          >  1:177351/1‑140 (MQ=255)                                                                       tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAg                                                        >  2:977214/1‑140 (MQ=255)                                                                         tccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTg                                                      >  1:174896/1‑140 (MQ=255)                                                                                taGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATg                                               <  1:802509/140‑1 (MQ=255)                                                                                             tttACGAATACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGc                                  >  2:1117131/1‑140 (MQ=255)                                                                                                    aTACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCgg                           <  2:1078727/140‑1 (MQ=255)                                                                                                     tACGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGc                          <  1:977214/140‑1 (MQ=255)                                                                                                      aCGGCGGATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCa                         <  2:201405/140‑1 (MQ=255)                                                                                                       cggcggATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAg                        >  1:168838/1‑140 (MQ=255)                                                                                                       cggcggATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAg                        >  1:554692/1‑140 (MQ=255)                                                                                                        ggcggATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGc                          >  1:201862/1‑137 (MQ=255)                                                                                                        ggcggATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGc                          <  2:201862/137‑1 (MQ=255)                                                                                                            gATCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGt                   >  2:1019418/1‑140 (MQ=255)                                                                                                             aTCGTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTa                  >  2:194081/1‑140 (MQ=255)                                                                                                                gTCACGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTg               >  2:1111767/1‑140 (MQ=255)                                                                                                                   aCGTCTACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTc            <  1:486531/140‑1 (MQ=255)                                                                                                                        tACAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCAt       >  2:1066973/1‑140 (MQ=255)                                                                                                                          cAGTACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCa     <  2:174896/140‑1 (MQ=255)                                                                                                                             tACGGCCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAggg  <  2:102868/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       GCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGG  >  NC_000913/4639585‑4639848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑