Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,283	A→G	N17N (AAT→AAC)	yaiY ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,283	0	A	G	100.0%	280.5 / NA	76	N17N (AAT→AAC)	yaiY	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/34);  total (42/34)

GACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATT  >  NC_000913/399147‑399412                                                                                                                                         |                                                                                                                                  gACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTg                                                                                                                                <  2:1053478/140‑1 (MQ=255)   ccTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCg                                                                                                                              >  2:923857/1‑140 (MQ=255)   ccTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCg                                                                                                                              >  2:127021/1‑140 (MQ=255)             ggACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCg                                                                                                                    >  1:152946/1‑140 (MQ=255)             ggACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCg                                                                                                                    <  2:152946/140‑1 (MQ=255)                  tGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTaaa                                                                                                               <  1:590327/140‑1 (MQ=255)                  tGCATCAAACGCTCATAGACGCCGGACGCATGCGCTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCCAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGACGCGTTGCGATATTTTCCGCTaaa                                                                                                               >  2:574135/1‑140 (MQ=255)                   gCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAGAc                                                                                                              >  2:752059/1‑140 (MQ=255)                    cATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACa                                                                                                             <  1:1003960/140‑1 (MQ=255)                    cATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACa                                                                                                             >  2:799902/1‑140 (MQ=255)                    cATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACa                                                                                                             <  1:752059/140‑1 (MQ=255)                     aTTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAg                                                                                                            <  2:468560/140‑1 (MQ=255)                              tCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGa                                                                                                   >  2:588446/1‑140 (MQ=255)                              tCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGa                                                                                                   >  2:352650/1‑140 (MQ=255)                              tCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGa                                                                                                   >  2:111274/1‑140 (MQ=255)                              tCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGa                                                                                                   >  1:894770/1‑140 (MQ=255)                              tCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGAACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGa                                                                                                   >  1:1070214/1‑140 (MQ=255)                               cATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGAc                                                                                                  <  1:352650/140‑1 (MQ=255)                               cATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGAc                                                                                                  >  1:872384/1‑140 (MQ=255)                                      gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACa                                                                                                             <  2:909630/122‑1 (MQ=255)                                      gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACa                                                                                                             >  1:909630/1‑122 (MQ=255)                                      gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGa                                                                                           >  2:247111/1‑140 (MQ=255)                                      gCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGa                                                                                           >  2:632667/1‑140 (MQ=255)                                        cGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGaaa                                                                                         >  2:1144917/1‑140 (MQ=255)                                        cGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGaaa                                                                                         >  2:202791/1‑140 (MQ=255)                                        cGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGaaa                                                                                         >  1:763475/1‑140 (MQ=255)                                        cGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGaaa                                                                                         >  2:10403/1‑140 (MQ=255)                                          ggCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATc                                                                                       <  1:202791/140‑1 (MQ=255)                                           gcgcATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCa                                                                                      <  1:664194/140‑1 (MQ=255)                                            cgcATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAg                                                                                     >  1:791266/1‑140 (MQ=255)                                            cgcATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAg                                                                                     <  1:10403/140‑1 (MQ=255)                                                   aCTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATaaa                                                                              <  1:632667/140‑1 (MQ=255)                                                    cTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATaaaa                                                                             <  1:247111/140‑1 (MQ=255)                                                     tAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATaaaaa                                                                            <  1:1144917/140‑1 (MQ=255)                                                       aCAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAAcc                                                                          >  2:853827/1‑140 (MQ=255)                                                          ggTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTcc                                                                       >  2:759309/1‑140 (MQ=255)                                                           gTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCg                                                                      >  1:204170/1‑140 (MQ=255)                                                           gTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATCTTGACAGGGTGAAATCAGCCATAAAAACCTCCg                                                                      <  1:1144908/140‑1 (MQ=255)                                                            ttCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCgg                                                                     >  2:1103872/1‑140 (MQ=255)                                                             tCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGa                                                                    >  2:710214/1‑140 (MQ=255)                                                             tCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGa                                                                    >  1:945579/1‑140 (MQ=255)                                                             tCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGa                                                                    >  1:805360/1‑140 (MQ=255)                                                             tCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGa                                                                    >  1:502496/1‑140 (MQ=255)                                                               aGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAg                                                                  >  2:1033614/1‑140 (MQ=255)                                                               aGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAg                                                                  >  2:567326/1‑140 (MQ=255)                                                                   aTTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCAt                                                              >  1:912770/1‑140 (MQ=255)                                                                     tGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAAc                                                                           <  2:10660/125‑1 (MQ=255)                                                                     tGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAAc                                                                           >  1:10660/1‑125 (MQ=255)                                                                       cgcCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGc                                                          <  1:1103872/140‑1 (MQ=255)                                                                         ccccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                        >  1:110441/1‑140 (MQ=255)                                                                         ccccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                        <  1:923857/140‑1 (MQ=255)                                                                         ccccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGctt                                                        <  1:853827/140‑1 (MQ=255)                                                                           ccAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttct                                                      <  1:1023087/140‑1 (MQ=255)                                                                              gCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctctt                                                   <  2:872384/140‑1 (MQ=255)                                                                               cccAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttc                                                  <  1:567326/140‑1 (MQ=255)                                                                               cccAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttc                                                  <  2:502496/140‑1 (MQ=255)                                                                                ccAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGcttctcttct                                                 <  2:280547/140‑1 (MQ=255)                                                                                         aaaCAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATa                                        >  2:679784/1‑140 (MQ=255)                                                                                             aGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAg                                    <  2:110441/140‑1 (MQ=255)                                                                                               cacaAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCg                                  >  1:181023/1‑140 (MQ=255)                                                                                                 caaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGc                                >  1:413244/1‑140 (MQ=255)                                                                                                  aaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCt                               <  2:543350/140‑1 (MQ=255)                                                                                                  aaacaaCGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCt                               <  2:666939/140‑1 (MQ=255)                                                                                                       acGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATgg                          <  2:310905/140‑1 (MQ=255)                                                                                                            tAGGCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGt                     <  2:894770/140‑1 (MQ=255)                                                                                                              ggCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCt                   >  2:364166/1‑140 (MQ=255)                                                                                                              ggCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCt                   >  2:870173/1‑140 (MQ=255)                                                                                                               gCAATGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTg                  <  1:799902/140‑1 (MQ=255)                                                                                                                 aaTGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGAc                <  2:805360/140‑1 (MQ=255)                                                                                                                  aTGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACa               <  2:1195622/140‑1 (MQ=255)                                                                                                                   tGTTGCCAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAg              >  2:1134429/1‑140 (MQ=255)                                                                                                                        ccAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAg              <  1:422918/135‑1 (MQ=255)                                                                                                                        ccAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAg              >  2:422918/1‑135 (MQ=255)                                                                                                                         cAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCAtt        >  2:1096858/1‑140 (MQ=255)                                                                                                                         cAGGCGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCAtt        >  1:657410/1‑140 (MQ=255)                                                                                                                             cGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGa    <  1:710214/140‑1 (MQ=255)                                                                                                                             cGTTGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGa    <  2:204170/140‑1 (MQ=255)                                                                                                                               ttGAAGAGGCGTTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGAtt  >  1:65716/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  GACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATTTTCCGCTAAACAGCGATTTTGACAGGGTGAAATCAGCCATAAAAACCTCCGGAAGTCATCTGCTTCTCTTCTGGTGTGATACCAGCGGCTTATGGCAAGTCTGACAGGTCATTTAGATT  >  NC_000913/399147‑399412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑