Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	452,876	C→T	A224T (GCC→ACC)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	452,876	0	C	T	100.0%	254.1 / NA	74	A224T (GCC→ACC)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/39);  total (35/39)

AGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAAC  >  NC_000913/452746‑453012                                                                                                                                   |                                                                                                                                         aGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCg                                                                                                                                 >  1:827052/1‑140 (MQ=255) aGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCg                                                                                                                                 >  2:891517/1‑140 (MQ=255) aGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTGGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCg                                                                                                                                 >  1:1071422/1‑140 (MQ=255)       ttGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGTGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCg                                                                                                                                 >  2:284427/1‑134 (MQ=255)       ttGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGTGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCg                                                                                                                                 <  1:284427/134‑1 (MQ=255)                aCGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCg                                                                                                                  >  1:489525/1‑140 (MQ=255)                aCGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCg                                                                                                                  <  1:388164/140‑1 (MQ=255)                  gCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCa                                                                                                                <  1:767534/140‑1 (MQ=255)                         tCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGc                                                                                                         >  1:69932/1‑140 (MQ=255)                         tCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGc                                                                                                         >  2:347092/1‑140 (MQ=255)                         tCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTC‑TGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCag                                                                                                               <  2:190262/133‑1 (MQ=255)                         tCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTC‑TGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCag                                                                                                               >  1:190262/1‑133 (MQ=255)                            cccGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAgg                                                                                                            >  2:233214/1‑134 (MQ=255)                            cccGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAgg                                                                                                            <  1:233214/134‑1 (MQ=255)                                cATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAAcc                                                                                                  >  2:623099/1‑140 (MQ=255)                                  tCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGc                                                                                                >  1:1083358/1‑140 (MQ=255)                                         cGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTcc                                                                                         <  2:489525/140‑1 (MQ=255)                                           aCGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGcaacaa                                                                                                    <  1:372585/127‑1 (MQ=255)                                           aCGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGcaacaa                                                                                                    >  2:372585/1‑127 (MQ=255)                                               cgcgAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGtt                                                                                   >  1:706771/1‑140 (MQ=255)                                               cgcgAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGtt                                                                                   >  2:1211285/1‑140 (MQ=255)                                                 cgAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGtttt                                                                                 <  2:827052/140‑1 (MQ=255)                                                      cAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGca                                                                            <  2:706771/140‑1 (MQ=255)                                                      cAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGca                                                                            <  1:1211285/140‑1 (MQ=255)                                                       aaaaaCGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGcac                                                                           <  1:623099/140‑1 (MQ=255)                                                              tGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGCCCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATg                                                                    >  2:964476/1‑140 (MQ=255)                                                                gTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCCGAGGTGCAACTACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGgt                                                                  >  2:864518/1‑140 (MQ=255)                                                                      aGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggt                                                            <  2:69932/140‑1 (MQ=255)                                                                      aGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggt                                                            <  1:477112/140‑1 (MQ=255)                                                                          tCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggttggt                                                        >  2:650604/1‑140 (MQ=255)                                                                           cATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCggttggtt                                                       <  2:949013/140‑1 (MQ=255)                                                                           cATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTAGGCACAGGAATGGTGTCggttggtt                                                       >  2:288946/1‑140 (MQ=255)                                                                              ggCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTAGGCACAGGAATGGTGTCGGTTGGTTCTg                                                    <  1:288946/140‑1 (MQ=255)                                                                                  aaTGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTgc                                                >  1:388996/1‑140 (MQ=255)                                                                                   aTGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTgca                                               <  1:650604/140‑1 (MQ=255)                                                                                    tGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTgcaa                                              >  1:980242/1‑140 (MQ=255)                                                                                           cccAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGACATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  2:1071422/140‑1 (MQ=255)                                                                                           cccAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       >  1:360553/1‑140 (MQ=255)                                                                                           cccAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  1:1090353/140‑1 (MQ=255)                                                                                           cccAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  2:1025572/140‑1 (MQ=255)                                                                                                     aCAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGataata                             >  1:731195/1‑140 (MQ=255)                                                                                                      cAGCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATAc                            <  1:1206602/140‑1 (MQ=255)                                                                                                        gCACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAg                          <  2:980242/140‑1 (MQ=255)                                                                                                         cACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       >  1:948564/1‑126 (MQ=255)                                                                                                         cACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  2:948564/126‑1 (MQ=255)                                                                                                         cACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGa                                  >  1:562144/1‑131 (MQ=255)                                                                                                         cACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGa                                  <  2:562144/131‑1 (MQ=255)                                                                                                         cACGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAgg                         >  2:157023/1‑140 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       >  1:194900/1‑125 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  1:598167/125‑1 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       >  2:598167/1‑125 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGt                                       <  2:194900/125‑1 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTcg                                     <  2:482095/127‑1 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTcg                                     >  1:482095/1‑127 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTcg                                     >  1:693856/1‑127 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTcg                                     <  2:693856/127‑1 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGa                                  >  1:762934/1‑130 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGa                                  <  2:762934/130‑1 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAggg                        >  1:208459/1‑140 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAggg                        >  2:937156/1‑140 (MQ=255)                                                                                                          aCGATAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAggg                        >  2:827447/1‑140 (MQ=255)                                                                                                              tAAGAAGCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATc                    >  1:631926/1‑140 (MQ=255)                                                                                                                agaagCAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGAACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCaa                  <  1:827447/140‑1 (MQ=255)                                                                                                                     cAAAATAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTg             <  2:495081/140‑1 (MQ=255)                                                                                                                      aaaaTAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTgc            <  1:675867/140‑1 (MQ=255)                                                                                                                      aaaaTAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTgc            <  1:275629/140‑1 (MQ=255)                                                                                                                        aaTAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTgccg          <  1:891517/140‑1 (MQ=255)                                                                                                                        aaTAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTgccg          >  2:379551/1‑140 (MQ=255)                                                                                                                          tAAGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTgccgcc        <  1:347092/140‑1 (MQ=255)                                                                                                                           aaGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCa       <  1:863584/140‑1 (MQ=255)                                                                                                                           aaGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCa       <  2:631926/140‑1 (MQ=255)                                                                                                                           aaGCCAGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCa       <  1:379551/140‑1 (MQ=255)                                                                                                                                aGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAAc  <  1:937156/140‑1 (MQ=255)                                                                                                                                aGGTATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAAc  <  1:568730/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         AGCGTTTTGTTAACCACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAAC  >  NC_000913/452746‑453012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑