Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,381	C→T	G675S (GGT→AGT)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,381	0	C	T	100.0%	346.1 / NA	96	G675S (GGT→AGT)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/48);  total (48/48)

ACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAG  >  NC_000913/482244‑482514                                                                                                                                          |                                                                                                                                      aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCa                                                                                                                                     >  1:277300/1‑140 (MQ=255) aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAATACTCa                                                                                                                                     >  2:427613/1‑140 (MQ=255)   cGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGt                                                                                                                                   >  2:870641/1‑140 (MQ=255)     tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTc                                                                                                                                 >  2:131986/1‑140 (MQ=255)     tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTc                                                                                                                                 >  2:457501/1‑140 (MQ=255)     tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTc                                                                                                                                 >  2:424033/1‑140 (MQ=255)     tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTc                                                                                                                                 >  1:894251/1‑140 (MQ=255)     tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTc                                                                                                                                 >  2:268837/1‑140 (MQ=255)       tGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCa                                                                                                                               >  1:1115420/1‑140 (MQ=255)         gACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACg                                                                                                                             >  2:281340/1‑140 (MQ=255)            gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGAtt                                                                                                                          <  1:341093/140‑1 (MQ=255)               cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCg                                                                                                                       <  2:195063/140‑1 (MQ=255)               cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCg                                                                                                                       <  2:162847/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  2:3830/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     >  1:951003/1‑140 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  1:457501/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  1:966020/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  2:1115420/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  2:951003/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCggg                                                                                                                     <  2:894251/140‑1 (MQ=255)                 cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAATACTCAGTTCCACGATTGCggg                                                                                                                     <  1:427613/140‑1 (MQ=255)                   ggTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCa                                                                                                                   >  1:176171/1‑140 (MQ=255)                    gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAg                                                                                                                  >  1:450731/1‑140 (MQ=255)                    gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAg                                                                                                                  >  1:452213/1‑140 (MQ=255)                        aCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTa                                                                                                              <  1:131986/140‑1 (MQ=255)                         cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTaa                                                                                                             <  2:277300/140‑1 (MQ=255)                            atCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAgg                                                                                                          >  1:969355/1‑140 (MQ=255)                             tCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGc                                                                                                         >  2:715516/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  2:530568/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  1:1101662/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  1:1102749/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  2:420390/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  2:216131/1‑140 (MQ=255)                              cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                                                                        >  2:461468/1‑140 (MQ=255)                                   tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc                                                                                                   >  1:458190/1‑140 (MQ=255)                                   tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAAc                                                                                                   >  2:156891/1‑140 (MQ=255)                                     cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCa                                                                                                 >  1:961271/1‑140 (MQ=255)                                      ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt                                                                                                <  2:1101662/140‑1 (MQ=255)                                      ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt                                                                                                <  2:1102749/140‑1 (MQ=255)                                          ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCa                                                                                            >  2:617811/1‑140 (MQ=255)                                                cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTg                                                                                      <  1:715516/140‑1 (MQ=255)                                                     agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTg                                                                                 >  2:850623/1‑140 (MQ=255)                                                     agcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTg                                                                                 <  1:148965/140‑1 (MQ=255)                                                      gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGc                                                                                <  2:685515/140‑1 (MQ=255)                                                         aCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgag                                                                             <  2:969355/140‑1 (MQ=255)                                                          cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgaga                                                                            >  1:464280/1‑140 (MQ=255)                                                          cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgaga                                                                            <  1:268837/140‑1 (MQ=255)                                                                aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                                                                      >  1:974819/1‑140 (MQ=255)                                                                aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                                                                      >  1:433761/1‑140 (MQ=255)                                                                    gcCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCgtgt                                                                  <  1:67513/140‑1 (MQ=255)                                                                     ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGtt                                                                 >  2:1075068/1‑140 (MQ=255)                                                                      cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                                                                      >  1:48785/1‑134 (MQ=255)                                                                      cTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                                                                      <  2:48785/134‑1 (MQ=255)                                                                       tGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGc                                                               >  1:273005/1‑140 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  2:450731/140‑1 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  2:974819/140‑1 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  1:617811/140‑1 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  1:530568/140‑1 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  1:461468/140‑1 (MQ=255)                                                                        gAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  1:156891/140‑1 (MQ=255)                                                                         aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGGCGGTTGCAGTGCTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAATCCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCAc                                                             <  2:191873/140‑1 (MQ=255)                                                                         aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCAc                                                             <  2:464280/140‑1 (MQ=255)                                                                         aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCAc                                                             <  2:213151/140‑1 (MQ=255)                                                                          gTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACg                                                            <  1:1075068/140‑1 (MQ=255)                                                                            cAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCATGTTGCACGCa                                                          <  1:420390/140‑1 (MQ=255)                                                                              gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              >  2:288866/1‑134 (MQ=255)                                                                              gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCa                                                              <  1:288866/134‑1 (MQ=255)                                                                                   tCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAAt                                                   <  1:870641/140‑1 (MQ=255)                                                                                   tCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAAt                                                   >  2:493584/1‑140 (MQ=255)                                                                                      tGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGc                                                <  1:216131/140‑1 (MQ=255)                                                                                             ggCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACt                                         >  2:120886/1‑140 (MQ=255)                                                                                                     tGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttc                                 >  2:1128585/1‑140 (MQ=255)                                                                                                       gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttctt                               >  2:903872/1‑140 (MQ=255)                                                                                                       gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttctt                               >  2:153763/1‑140 (MQ=255)                                                                                                        tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttcttc                              >  1:554629/1‑140 (MQ=255)                                                                                                        tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttcttc                              >  2:946689/1‑140 (MQ=255)                                                                                                        tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTttcttc                              >  2:1077641/1‑140 (MQ=255)                                                                                                        tCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAACCGCTTCAACTTTGTTttcttc                              >  2:1216585/1‑140 (MQ=255)                                                                                                          aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCtt                                              >  1:84455/1‑122 (MQ=255)                                                                                                          aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCtt                                              <  2:84455/122‑1 (MQ=255)                                                                                                             cAGCTCAAAGTCAAAGCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCg                         >  1:200950/1‑140 (MQ=255)                                                                                                                 tcaaagtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCgg                        <  2:1073766/137‑1 (MQ=255)                                                                                                                 tcaaagtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCgg                        >  1:1073766/1‑137 (MQ=255)                                                                                                                  caaagtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGa                    >  2:278155/1‑140 (MQ=255)                                                                                                                   aaagtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGAt                   <  1:944461/140‑1 (MQ=255)                                                                                                                   aaagtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGAt                   <  1:120886/140‑1 (MQ=255)                                                                                                                      gtcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCgg                <  1:903872/140‑1 (MQ=255)                                                                                                                       tcaaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGc               <  2:176171/140‑1 (MQ=255)                                                                                                                        caaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGcc              <  1:850623/140‑1 (MQ=255)                                                                                                                         aaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGccc             <  2:273005/140‑1 (MQ=255)                                                                                                                         aaagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGccc             <  1:869582/140‑1 (MQ=255)                                                                                                                          aagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCa            <  2:554629/140‑1 (MQ=255)                                                                                                                          aagCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCa            >  2:860206/1‑140 (MQ=255)                                                                                                                           agCCGGTTGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAg           <  1:153763/140‑1 (MQ=255)                                                                                                                                  tGCAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCa    <  1:281340/140‑1 (MQ=255)                                                                                                                                    cAGTACTCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAg  <  1:946689/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      ACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGGTAATCGCTTCAACTTTGTTTTCTTCGCCCGGACGATCGGCCCAGTCCTTCAAG  >  NC_000913/482244‑482514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑