Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,208	T→C	G284G (GGT→GGC)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,208	0	T	C	100.0%	195.6 / NA	53	G284G (GGT→GGC)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/23);  total (30/23)

GCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTG  >  NC_000913/549074‑549341                                                                                                                                       |                                                                                                                                      gcgATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGtt                                                                                                                                  >  2:347209/1‑140 (MQ=255)             ggCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCtt                                                                                                                      <  2:607906/140‑1 (MQ=255)              gcgcGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTCTTCACCGGCTtc                                                                                                                     >  1:317476/1‑140 (MQ=255)               cgcgATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTtct                                                                                                                    >  1:414368/1‑140 (MQ=255)                           cgcAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                                                                        <  1:637437/140‑1 (MQ=255)                            gcaggcagCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGc                                                                                                       <  2:441002/140‑1 (MQ=255)                            gcaggcagCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGc                                                                                                       <  1:710352/140‑1 (MQ=255)                                gcagcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                                                                        >  1:933100/1‑135 (MQ=255)                                gcagcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                                                                        <  2:933100/135‑1 (MQ=255)                                  agcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAAccc                                                                                                 >  1:805205/1‑140 (MQ=255)                                  agcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACCGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAAccc                                                                                                 >  2:265973/1‑140 (MQ=255)                                    caTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCgg                                                                                               <  2:765960/140‑1 (MQ=255)                                    caTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCgg                                                                                               <  2:836483/140‑1 (MQ=255)                                    caTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCgg                                                                                               <  2:1140678/140‑1 (MQ=255)                                    caTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTCTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCgg                                                                                               <  2:317476/140‑1 (MQ=255)                                          cACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATgg                                                                                         >  2:813053/1‑140 (MQ=255)                                             ggCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCg                                                                                      <  2:805205/140‑1 (MQ=255)                                              gCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGa                                                                                     >  2:962857/1‑140 (MQ=255)                                                   gACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAgcg                                                                                <  2:1188007/140‑1 (MQ=255)                                                     cccGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGcgcg                                                                              <  1:962857/140‑1 (MQ=255)                                                      ccGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGa                                                                             <  1:952917/140‑1 (MQ=255)                                                          aaCGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTAc                                                                         >  2:1198904/1‑140 (MQ=255)                                                              gCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGaa                                                                     <  2:414368/140‑1 (MQ=255)                                                               cAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGaaa                                                                    <  2:157682/140‑1 (MQ=255)                                                               cAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTG‑TTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAAc                                                                   >  1:584308/1‑140 (MQ=255)                                                                  tatGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCt                                                                 <  1:1198904/140‑1 (MQ=255)                                                                       tCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGt                                                            >  2:986288/1‑140 (MQ=255)                                                                         gggATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTAt                                                          >  2:446412/1‑140 (MQ=255)                                                                         gggATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTAt                                                          >  1:222740/1‑140 (MQ=255)                                                                            aTTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCgg                                                       <  1:986288/140‑1 (MQ=255)                                                                              tCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAg                                                     <  2:602114/140‑1 (MQ=255)                                                                              tCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAg                                                     >  1:623133/1‑140 (MQ=255)                                                                                gggTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGt                                                   >  2:605658/1‑140 (MQ=255)                                                                                     aGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGCAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGc                                              >  2:1085243/1‑140 (MQ=255)                                                                                           cTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGAt                                        >  2:194559/1‑140 (MQ=255)                                                                                              ggCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGc                                     <  1:505097/140‑1 (MQ=255)                                                                                                   aCGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAgcgc                                >  2:1077913/1‑140 (MQ=255)                                                                                                               tGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAAcgc                    >  1:222907/1‑140 (MQ=255)                                                                                                               tGCGCCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAAcgc                    >  1:606364/1‑140 (MQ=255)                                                                                                                gcgcCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAAcgcg                   <  1:813053/140‑1 (MQ=255)                                                                                                                 cgcCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAgcgcgc                  >  2:1084140/1‑140 (MQ=255)                                                                                                                 cgcCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACgcgc                  >  2:185797/1‑140 (MQ=255)                                                                                                                 cgcCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACgcgc                  >  2:1134170/1‑140 (MQ=255)                                                                                                                  gcCTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCt                 <  1:1134170/140‑1 (MQ=255)                                                                                                                   ccTGTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCtt                >  2:771156/1‑140 (MQ=255)                                                                                                                      gTAAACACTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGt             <  1:1077913/140‑1 (MQ=255)                                                                                                                           cacTCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTg        <  1:194559/140‑1 (MQ=255)                                                                                                                              tCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCgg     >  1:289615/1‑140 (MQ=255)                                                                                                                              tCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCACAGCGCCTGGCGTAACGCGCTTTGTCGGTGCgg     >  1:88615/1‑140 (MQ=255)                                                                                                                              tCCGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGGGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCgg     >  2:726209/1‑140 (MQ=255)                                                                                                                                cGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGt   >  1:52392/1‑140 (MQ=255)                                                                                                                                cGCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGt   >  1:486397/1‑140 (MQ=255)                                                                                                                                 gCAAGGCCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTg  >  2:1089774/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATCGGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTG  >  NC_000913/549074‑549341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑