Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 626,394 C→T R109C (CGT→TGT)  entE → 2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913626,3940CT100.0% 164.3 / NA 47R109C (CGT→TGT) entE2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/23);  total (24/23)

TAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCG  >  NC_000913/626258‑626520
                                                                                                                                        |                                                                                                                              
tAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTa                                                                                                                             <  1:163964/140‑1 (MQ=255)
         ccGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCACCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGtgaactga                                                                                                                    <  1:1055500/140‑1 (MQ=255)
             cAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGc                                                                                                                <  2:1146490/140‑1 (MQ=255)
                            ggTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGAt                                                                                                 >  2:89954/1‑140 (MQ=255)
                                       gcTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCAt                                                                                      >  2:609632/1‑140 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGa                                                                         <  2:360262/139‑1 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGa                                                                         >  1:360262/1‑139 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGAt                                                                        >  2:650639/1‑140 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGAt                                                                        >  1:445535/1‑140 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGAt                                                                        >  2:247439/1‑140 (MQ=255)
                                                     gTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGAt                                                                        >  2:105776/1‑140 (MQ=255)
                                                      tAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATc                                                                       >  1:585681/1‑140 (MQ=255)
                                                       aaCGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCg                                                                      <  1:247439/140‑1 (MQ=255)
                                                         cGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGcc                                                                    <  2:585681/140‑1 (MQ=255)
                                                                     gTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGt                                                        >  2:1048424/1‑140 (MQ=255)
                                                                      tataTTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGtt                                                       >  2:716135/1‑140 (MQ=255)
                                                                          ttACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGc                                                   <  1:798103/140‑1 (MQ=255)
                                                                           tACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCg                                                  >  1:1071064/1‑140 (MQ=255)
                                                                           tACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCg                                                  >  2:256684/1‑140 (MQ=255)
                                                                             ccTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATgcg                                                            <  2:463173/128‑1 (MQ=255)
                                                                             ccTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATgcg                                                            >  1:463173/1‑128 (MQ=255)
                                                                             ccTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCggg                                                >  1:1204802/1‑140 (MQ=255)
                                                                             ccTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCggg                                                <  2:445535/140‑1 (MQ=255)
                                                                             ccTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCggg                                                >  2:1107548/1‑140 (MQ=255)
                                                                              cTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCgggg                                               >  1:267127/1‑140 (MQ=255)
                                                                                ttttCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgat                                             >  2:665881/1‑140 (MQ=255)
                                                                                ttttCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgat                                             >  1:808785/1‑140 (MQ=255)
                                                                                ttttCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAAAATGCGCTGTTTAGCGGGgat                                             >  2:315652/1‑140 (MQ=255)
                                                                                ttttCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATCGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgat                                             >  1:1033676/1‑140 (MQ=255)
                                                                                 tttCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgatg                                            <  1:665881/140‑1 (MQ=255)
                                                                                   tCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgatgat                                          <  2:267127/140‑1 (MQ=255)
                                                                                   tCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGgatgat                                          <  1:609632/140‑1 (MQ=255)
                                                                                            tgAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAAt                                 <  1:650639/140‑1 (MQ=255)
                                                                                                 cTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACttt                            <  1:716135/140‑1 (MQ=255)
                                                                                                     gCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCt                                     >  2:232827/1‑127 (MQ=255)
                                                                                                     gCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCt                                     <  1:232827/127‑1 (MQ=255)
                                                                                                     gCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTc                        >  2:402097/1‑140 (MQ=255)
                                                                                                     gCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTc                        >  2:404199/1‑140 (MQ=255)
                                                                                                        ttGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTcaca                     <  1:74271/140‑1 (MQ=255)
                                                                                                         tGCGCCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATCGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAg                    <  2:1033676/140‑1 (MQ=255)
                                                                                                          gcgcCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGa                   <  1:105776/140‑1 (MQ=255)
                                                                                                           cgcCGGTGCTGGCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGaa                  >  2:286777/1‑140 (MQ=255)
                                                                                                                      gCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCAt       <  1:306590/140‑1 (MQ=255)
                                                                                                                      gCGTTGTTCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCAt       <  1:61680/140‑1 (MQ=255)
                                                                                                                         ttgttCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTcg    <  1:404199/140‑1 (MQ=255)
                                                                                                                         ttgttCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTcg    <  2:1071064/140‑1 (MQ=255)
                                                                                                                           gttCAGCCATCAGTGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTcgcg  <  1:256684/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                              
TAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCG  >  NC_000913/626258‑626520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑