Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	656,991	C→T	I145I (ATC→ATT)	pagP →	Lipid IVA palmitoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	656,991	0	C	T	100.0%	252.7 / NA	72	I145I (ATC→ATT)	pagP	Lipid IVA palmitoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/38);  total (34/38)

TCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAA  >  NC_000913/656860‑657127                                                                                                                                    |                                                                                                                                         tCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTcc                                                                                                                                   <  2:882866/139‑1 (MQ=255) tCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTcc                                                                                                                                   >  1:882866/1‑139 (MQ=255)  cGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCg                                                                                                                                 >  1:1018376/1‑140 (MQ=255)   gTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCgg                                                                                                                                >  1:62141/1‑140 (MQ=255)   gTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCgg                                                                                                                                >  2:238129/1‑140 (MQ=255)    tGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGt                                                                                                                               >  1:556536/1‑140 (MQ=255)      gAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTc                                                                                                                             >  1:1196261/1‑140 (MQ=255)         cAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTAc                                                                                                                          <  2:47919/140‑1 (MQ=255)         cAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTAc                                                                                                                          <  1:238129/140‑1 (MQ=255)         cAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTAc                                                                                                                          <  2:1196261/140‑1 (MQ=255)                 aaCCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTg                                                                                                                  <  1:440607/140‑1 (MQ=255)                  aCCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTgg                                                                                                                 <  1:627604/140‑1 (MQ=255)                  aCCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTgg                                                                                                                 >  1:369982/1‑140 (MQ=255)                      aTTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTAc                                                                                                                          <  2:832049/127‑1 (MQ=255)                      aTTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTAc                                                                                                                          >  1:832049/1‑127 (MQ=255)                      aTTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTc                                                                                                             >  2:250509/1‑140 (MQ=255)                        tGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCg                                                                                                           >  2:427692/1‑140 (MQ=255)                        tGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCg                                                                                                           <  2:1209397/140‑1 (MQ=255)                               tACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTa                                                                                                    >  2:168623/1‑140 (MQ=255)                                aCGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTAt                                                                                                   <  2:193933/140‑1 (MQ=255)                                   gATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGc                                                                                                >  2:430595/1‑140 (MQ=255)                                        gAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGt                                                                                           >  1:63798/1‑140 (MQ=255)                                        gAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGt                                                                                           >  2:1219401/1‑140 (MQ=255)                                         aaaGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTg                                                                                          >  2:662560/1‑140 (MQ=255)                                             tACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACtt                                                                                      >  2:1058719/1‑140 (MQ=255)                                              aCCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACttt                                                                                     >  2:399376/1‑140 (MQ=255)                                                  ggCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCTTTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAg                                                                                 >  2:144356/1‑140 (MQ=255)                                                  ggCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAg                                                                                 >  2:1135135/1‑140 (MQ=255)                                                  ggCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAg                                                                                 >  2:267656/1‑140 (MQ=255)                                                  ggCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGGAACGGCACGGGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAg                                                                                 >  2:855545/1‑140 (MQ=255)                                                    cGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGAt                                                                               <  1:144356/140‑1 (MQ=255)                                                    cGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGAt                                                                               <  2:556536/140‑1 (MQ=255)                                                    cGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGAt                                                                               <  1:1135135/140‑1 (MQ=255)                                                    cGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGAt                                                                               <  2:369982/140‑1 (MQ=255)                                                      aCCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGa                                                                             >  1:153483/1‑140 (MQ=255)                                                         gCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCt                                                                          <  1:152656/140‑1 (MQ=255)                                                               ggATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAGCGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTc                                                                    <  1:1058719/140‑1 (MQ=255)                                                                  tGAATATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGCCCTACATTCCgg                                                                 <  2:485806/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  1:427692/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  2:62141/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  1:662560/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 >  2:1222736/1‑140 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  2:1018376/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  2:153483/140‑1 (MQ=255)                                                                  tGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  1:876757/140‑1 (MQ=255)                                                                           tCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  2:1136979/131‑1 (MQ=255)                                                                           tCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 >  1:1136979/1‑131 (MQ=255)                                                                            cATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTacaa                                                       <  1:267656/140‑1 (MQ=255)                                                                            cATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTacaa                                                       <  1:399376/140‑1 (MQ=255)                                                                                 aGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATg                                                  <  1:615520/140‑1 (MQ=255)                                                                                   gTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 >  2:1140880/1‑123 (MQ=255)                                                                                   gTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCgg                                                                 <  1:1140880/123‑1 (MQ=255)                                                                                   gTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGc                                                <  2:63798/140‑1 (MQ=255)                                                                                   gTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGc                                                <  1:729587/140‑1 (MQ=255)                                                                                      tGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAAt                                             <  2:1098702/140‑1 (MQ=255)                                                                                             cACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCa                                               >  2:495707/1‑131 (MQ=255)                                                                                             cACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCa                                               <  1:495707/131‑1 (MQ=255)                                                                                             cACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACt                                      <  1:1219401/140‑1 (MQ=255)                                                                                             cACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACt                                      >  1:278898/1‑140 (MQ=255)                                                                                              aCCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATg                                                  <  2:274286/127‑1 (MQ=255)                                                                                              aCCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATg                                                  >  1:274286/1‑127 (MQ=255)                                                                                              aCCGCTGGCGTAACGGCACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACtt                                     >  2:728454/1‑140 (MQ=255)                                                                                                  cTGGCGTAACGACACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTa                                        >  2:650938/1‑133 (MQ=255)                                                                                                  cTGGCGTAACGACACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTa                                        <  1:650938/133‑1 (MQ=255)                                                                                                   tGGCGTAACGGCACGCGATAACTGGAATTACATTCCACTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCt                                >  1:858860/1‑140 (MQ=255)                                                                                                              cACGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCttt                     <  2:1081897/140‑1 (MQ=255)                                                                                                               aCGCGATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTc                    >  2:644035/1‑140 (MQ=255)                                                                                                                   gATAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGtt                <  1:168623/140‑1 (MQ=255)                                                                                                                    aTAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGttt               >  2:735896/1‑140 (MQ=255)                                                                                                                     tAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGtttt              <  1:250509/140‑1 (MQ=255)                                                                                                                     tAACTGGAATTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGtttt              <  1:430595/140‑1 (MQ=255)                                                                                                                             aTTACATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACaaa      >  2:340402/1‑140 (MQ=255)                                                                                                                                 cATTCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGaa  <  1:340402/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TCGTGGAACAAATGGGAACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCGCTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCAGTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTGAGACAAATGAA  >  NC_000913/656860‑657127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑