Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,967	A→G	R120R (CGT→CGC)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,967	0	A	G	97.3%	259.0 / ‑2.9	75	R120R (CGT→CGC)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (40/33);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAG  >  NC_000913/699834‑700103                                                                                                                                      |                                                                                                                                         tctcAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGtt                                                                                                                                    <  1:719987/140‑1 (MQ=255)       tCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCca                                                                                                                              >  1:1174167/1‑140 (MQ=255)         ccTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaac                                                                                                                            <  2:1147680/140‑1 (MQ=255)           tGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaacaa                                                                                                                          >  1:290461/1‑140 (MQ=255)            gCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAAt                                                                                                                         >  2:494398/1‑140 (MQ=255)                 caACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           <  2:115648/133‑1 (MQ=255)                 caACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           >  1:115648/1‑133 (MQ=255)                 caACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGa                                                                                                                    >  1:204195/1‑140 (MQ=255)                  aaCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCca                                                                                                                              >  1:826964/1‑129 (MQ=255)                  aaCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCca                                                                                                                              <  2:826964/129‑1 (MQ=255)                  aaCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACgga                                                                                                                   >  1:425683/1‑138 (MQ=255)                   aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGaca                                                                                                                  >  1:1005000/1‑138 (MQ=255)                   aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGaaa                                                                                                                  >  2:225199/1‑140 (MQ=255)                   aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGaaa                                                                                                                  >  1:99141/1‑140 (MQ=255)                   aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGaaa                                                                                                                  >  2:538852/1‑140 (MQ=255)                   aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGaaa                                                                                                                  >  2:80270/1‑140 (MQ=255)                    cgcgCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAAt                                                                                                                 >  2:419984/1‑140 (MQ=255)                    cgcgCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAAt                                                                                                                 >  2:841997/1‑140 (MQ=255)                    cgcgCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAAt                                                                                                                 >  2:579783/1‑140 (MQ=255)                     gcgcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           <  2:219507/129‑1 (MQ=255)                     gcgcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           >  1:219507/1‑129 (MQ=255)                       gcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           >  2:9998/1‑127 (MQ=255)                       gcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCcaaca                                                                                                                           <  1:9998/127‑1 (MQ=255)                       gcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCa                                                                                                                       <  1:953500/131‑1 (MQ=255)                       gcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCa                                                                                                                       >  2:953500/1‑131 (MQ=255)                       gcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAg                                                                                                              >  1:813667/1‑140 (MQ=255)                         cacaAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAggg                                                                                                            <  1:331989/140‑1 (MQ=255)                            aaGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTc                                                                                                         >  2:746063/1‑140 (MQ=255)                              gCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTcac                                                                                                       <  2:221995/140‑1 (MQ=255)                               cGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGGGCACCGTTAGCGGCGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTcaca                                                                                                      <  2:495644/140‑1 (MQ=255)                                gggATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACAt                                                                                                     <  1:502125/140‑1 (MQ=255)                                gggATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACAt                                                                                                     <  1:494398/140‑1 (MQ=255)                                   aTAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATc                                                                                                                >  2:214528/1‑126 (MQ=255)                                   aTAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATc                                                                                                                <  1:214528/126‑1 (MQ=255)                                    tAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGt                                                                                                 <  1:80270/140‑1 (MQ=255)                                            gcgcCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGa                                                                                         >  2:400426/1‑140 (MQ=255)                                             cgcCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGaa                                                                                        >  2:460696/1‑140 (MQ=255)                                               cccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAAc                                                                                      <  1:841997/140‑1 (MQ=255)                                               cccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAAc                                                                                      <  1:746063/140‑1 (MQ=255)                                               cccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAAc                                                                                      <  1:225199/140‑1 (MQ=255)                                               cccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAAc                                                                                      <  2:99141/140‑1 (MQ=255)                                               cccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTTAAAc                                                                                      <  2:813667/140‑1 (MQ=255)                                                         tCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGGAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTa                                                                            <  2:425683/140‑1 (MQ=255)                                                         tCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTa                                                                            >  1:583434/1‑140 (MQ=255)                                                         tCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTa                                                                            <  1:400426/140‑1 (MQ=255)                                                           cGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACa                                                                          >  2:810188/1‑140 (MQ=255)                                                                        aTAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTc                                                                      >  1:1114385/1‑131 (MQ=255)                                                                        aTAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTc                                                                      <  2:1114385/131‑1 (MQ=255)                                                                         tAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGt                                                            <  2:204195/140‑1 (MQ=255)                                                                          aaaaCGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTg                                                           <  2:583434/140‑1 (MQ=255)                                                                            aaCGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACa                                                                          <  1:409683/123‑1 (MQ=255)                                                                            aaCGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACa                                                                          >  2:409683/1‑123 (MQ=255)                                                                                tGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATAAGTGCGCCtt                                                     >  2:126108/1‑140 (MQ=255)                                                                                 gCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTc                                                    >  1:206382/1‑140 (MQ=255)                                                                                  cACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGa                                                                  >  1:275042/1‑125 (MQ=255)                                                                                  cACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGa                                                                  <  2:275042/125‑1 (MQ=255)                                                                                   aCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGc                                                  <  1:810188/140‑1 (MQ=255)                                                                                    ccGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGcc                                                 <  1:419984/140‑1 (MQ=255)                                                                                     cGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGccc                                                <  2:443225/140‑1 (MQ=255)                                                                                       ttAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCccac                                              >  1:1132082/1‑140 (MQ=255)                                                                                       ttAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCccac                                              >  1:168566/1‑140 (MQ=255)                                                                                       ttAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCccac                                              >  1:589446/1‑140 (MQ=255)                                                                                             acgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATa                                        <  2:1005000/140‑1 (MQ=255)                                                                                             acgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATa                                        <  1:579783/140‑1 (MQ=255)                                                                                             acgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATa                                        >  2:206933/1‑140 (MQ=255)                                                                                                 aaTAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCt                                    <  2:206382/140‑1 (MQ=255)                                                                                                           tttATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCtt                          <  2:301186/140‑1 (MQ=255)                                                                                                           tttATGCATCATGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCtt                          >  1:301186/1‑140 (MQ=255)                                                                                                                    catGTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGAc                 >  1:684698/1‑140 (MQ=255)                                                                                                                       gTCCCAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATGGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCa              >  1:255302/1‑140 (MQ=255)                                                                                                                         cccAGTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAgg            >  2:7112/1‑140 (MQ=255)                                                                                                                             gTTGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAAt        <  2:1132082/140‑1 (MQ=255)                                                                                                                              ttGTAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATc       >  1:84756/1‑140 (MQ=255)                                                                                                                                 tAGGAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGc    >  2:148062/1‑140 (MQ=255)                                                                                                                                   ggAGCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAg  >  2:143591/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         TCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAG  >  NC_000913/699834‑700103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑