Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,022	C→T	P204S (CCG→TCG)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,022	0	C	T	100.0%	261.7 / NA	75	P204S (CCG→TCG)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/40);  total (35/40)

ATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCG  >  NC_000913/91887‑92151                                                                                                                                        |                                                                                                                                  aTTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCggg                                                                                                                               <  2:335045/140‑1 (MQ=255) aTTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCggg                                                                                                                               >  1:21154/1‑140 (MQ=255)  ttCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGt                                                                                                                              <  2:197610/140‑1 (MQ=255)         gCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCa                                                                                                                       <  2:53634/140‑1 (MQ=255)         gCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCa                                                                                                                       <  2:388489/140‑1 (MQ=255)         gCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCa                                                                                                                       <  1:409932/140‑1 (MQ=255)          cGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCAt                                                                                                                      >  2:614583/1‑140 (MQ=255)            tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATtg                                                                                                                    <  2:499911/140‑1 (MQ=255)            tGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATtg                                                                                                                    <  1:614583/140‑1 (MQ=255)               agagTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGc                                                                                                                 >  2:351200/1‑140 (MQ=255)                  gTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTa                                                                                                              <  2:825223/140‑1 (MQ=255)                    ctcGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTaaa                                                                                                            <  1:226377/140‑1 (MQ=255)                    ctcGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTaaa                                                                                                            >  1:1063364/1‑140 (MQ=255)                    ctcGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTaaa                                                                                                            <  2:479385/140‑1 (MQ=255)                     tcGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTaaaa                                                                                                           >  2:778926/1‑139 (MQ=255)                             aCTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTAt                                                                                                   >  1:807699/1‑140 (MQ=255)                             aCTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTAt                                                                                                   >  1:1180118/1‑140 (MQ=255)                                   cgtccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAg                                                                                                           <  1:541962/126‑1 (MQ=255)                                   cgtccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAg                                                                                                           >  2:541962/1‑126 (MQ=255)                                    gtccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg                                                                                            <  1:351200/140‑1 (MQ=255)                                      ccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTa                                                                                          >  2:922627/1‑140 (MQ=255)                                      ccgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGAGCGTAAAGACCGCTATGGTCGCGTa                                                                                          >  2:638879/1‑140 (MQ=255)                                       cgGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTaa                                                                                         >  2:32984/1‑140 (MQ=255)                                         gCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGGTTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAtt                                                                                       >  2:1177993/1‑140 (MQ=255)                                         gCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg                                                                                            >  1:304710/1‑135 (MQ=255)                                         gCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg                                                                                            <  2:304710/135‑1 (MQ=255)                                            aaGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGaa                                                                                    <  2:21154/140‑1 (MQ=255)                                               tGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGat                                                                                 <  2:1164069/140‑1 (MQ=255)                                                 aCTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGatat                                                                               <  2:1180118/140‑1 (MQ=255)                                                   tGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGCCCGCTATGGTCGCGTAATTGAAGATAttt                                                                             <  1:32984/140‑1 (MQ=255)                                                   tGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATAttt                                                                             <  1:539719/140‑1 (MQ=255)                                                   tGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATAttt                                                                             <  1:534151/140‑1 (MQ=255)                                                   tGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGAGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATAttt                                                                             <  1:638879/140‑1 (MQ=255)                                                      tCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATttctt                                                                          >  2:1013032/1‑140 (MQ=255)                                                        aCCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTtcttct                                                                        >  2:988596/1‑140 (MQ=255)                                                         ccTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTa                                                                       >  2:378883/1‑140 (MQ=255)                                                            cATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTg                                                                    <  1:1129183/140‑1 (MQ=255)                                                               cGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACa                                                                 >  2:828649/1‑140 (MQ=255)                                                                  cTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGatat                                                                               >  2:58921/1‑123 (MQ=255)                                                                  cTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGatat                                                                               <  1:58921/123‑1 (MQ=255)                                                                    ttACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAg                                                            >  1:432648/1‑140 (MQ=255)                                                                          aCGTCGATAGTCAAGGGATTGACGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGACGGGGGAGCGCATTGTGCGTAAAGACCGCTATGATCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAgcc                                                      >  2:876067/1‑139 (MQ=255)                                                                           cGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAgcgc                                                     >  1:911598/1‑140 (MQ=255)                                                                            gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGca                                                    >  2:20326/1‑140 (MQ=255)                                                                            gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGca                                                    <  1:1013032/140‑1 (MQ=255)                                                                            gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGca                                                    <  1:1006991/140‑1 (MQ=255)                                                                            gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGca                                                    <  1:378883/140‑1 (MQ=255)                                                                            gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGca                                                    <  1:922627/140‑1 (MQ=255)                                                                                aTAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAAc                                                >  2:461291/1‑140 (MQ=255)                                                                                 tAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAAcc                                               <  1:333720/140‑1 (MQ=255)                                                                                   gTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTg                                             <  2:807699/140‑1 (MQ=255)                                                                                      aaGGGGTTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGgcg                                          <  1:1177993/140‑1 (MQ=255)                                                                                      aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGgcg                                          <  2:911598/140‑1 (MQ=255)                                                                                        gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        >  1:965697/1‑140 (MQ=255)                                                                                        gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        <  2:884084/140‑1 (MQ=255)                                                                                        gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        <  2:1049477/140‑1 (MQ=255)                                                                                        gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        >  1:592708/1‑140 (MQ=255)                                                                                             tGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTa                                   >  1:254362/1‑140 (MQ=255)                                                                                              gAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        <  2:1100072/134‑1 (MQ=255)                                                                                              gAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCt                                        >  1:1100072/1‑134 (MQ=255)                                                                                                   cGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATtgatg                             >  1:810126/1‑140 (MQ=255)                                                                                                    gTTGTGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATtgatga                            >  1:55168/1‑140 (MQ=255)                                                                                                    gTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTg                                             <  2:505013/123‑1 (MQ=255)                                                                                                    gTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTg                                             >  1:505013/1‑123 (MQ=255)                                                                                                    gTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATtgatga                            >  1:610228/1‑140 (MQ=255)                                                                                                    gTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATCTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATtgatga                            >  1:539622/1‑140 (MQ=255)                                                                                                               tttCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAgg                 >  1:1133256/1‑140 (MQ=255)                                                                                                               tttCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAgg                 <  1:988596/140‑1 (MQ=255)                                                                                                                 tCGATAAATGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcg               >  1:741526/1‑140 (MQ=255)                                                                                                                        aTGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGttt        >  2:416376/1‑140 (MQ=255)                                                                                                                         tGGCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGATAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTa       >  1:1118074/1‑140 (MQ=255)                                                                                                                           gCTTACCGGGTAGTCGGGTGAGCTCATTGTTCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGAGGAACGCCTGCAGGCGCTGGTTTATc     <  2:810126/140‑1 (MQ=255)                                                                                                                           gCTTACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATc     <  1:791078/140‑1 (MQ=255)                                                                                                                              tACCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATcgcg  <  2:1063364/140‑1 (MQ=255)                                                                                                                               aCCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGt          >  2:628267/1‑131 (MQ=255)                                                                                                                               aCCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGt          <  1:628267/131‑1 (MQ=255)                                                                                                                               aCCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATcgcg  <  1:1223607/139‑1 (MQ=255)                                                                                                                               aCCGGGCAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATcgcg  >  2:1223607/1‑139 (MQ=255)                                                                                                                                     cAGTCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGt          <  2:661952/125‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCG  >  NC_000913/91887‑92151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑