Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	788,084	C→T	A252T (GCC→ACC)	galM ←	galactose‑1‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	788,084	0	C	T	100.0%	127.4 / NA	37	A252T (GCC→ACC)	galM	galactose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/21);  total (16/21)

GTTCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGCCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGACAGATTTC  >  NC_000913/787948‑788202                                                                                                                                         |                                                                                                                       gttcCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTg                                                                                                                     <  2:739937/140‑1 (MQ=255)  ttcCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGt                                                                                                                    <  2:885827/140‑1 (MQ=255)     cccGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAAc                                                                                                                 >  2:356961/1‑140 (MQ=255)      ccGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACa                                                                                                                >  2:464318/1‑140 (MQ=255)        gcgACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAg                                                                                                              <  1:223869/140‑1 (MQ=255)        gcgACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAg                                                                                                              <  2:91431/140‑1 (MQ=255)         cgACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGa                                                                                                             <  2:782074/140‑1 (MQ=255)              gtgtGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCg                                                                                                        <  1:681446/140‑1 (MQ=255)               tgtgCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGt                                                                                                       >  1:774012/1‑140 (MQ=255)               tgtgCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGt                                                                                                       <  2:103971/140‑1 (MQ=255)               tgtgCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGt                                                                                                       >  1:967446/1‑140 (MQ=255)                    cgccgAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCg                                                                                                  <  1:482963/140‑1 (MQ=255)                     gccgAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGt                                                                                                 >  2:40700/1‑140 (MQ=255)                     gccgAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGt                                                                                                 <  1:356961/140‑1 (MQ=255)                      ccgAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTa                                                                                                <  1:337225/140‑1 (MQ=255)                         aGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCg                                                                                                        <  1:97168/129‑1 (MQ=255)                         aGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCg                                                                                                        >  2:97168/1‑129 (MQ=255)                         aGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAAcc                                                                                             >  2:1148504/1‑140 (MQ=255)                         aGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAAcc                                                                                             >  1:1152415/1‑140 (MQ=255)                                tGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACt                                                                                      <  2:774012/140‑1 (MQ=255)                                tGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACt                                                                                      <  2:967446/140‑1 (MQ=255)                                 gCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACtt                                                                                     <  1:464318/140‑1 (MQ=255)                                      aGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACt                                                                                      <  1:259036/134‑1 (MQ=255)                                      aGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACt                                                                                      >  2:259036/1‑134 (MQ=255)                                                    gCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGc                                                                  <  1:1148504/140‑1 (MQ=255)                                                     ccGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCa                                                                 <  2:1171036/140‑1 (MQ=255)                                                                     gACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCga                                                 <  2:1152415/140‑1 (MQ=255)                                                                          tCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGAtt                                            >  2:407/1‑140 (MQ=255)                                                                          tCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGAtt                                            >  1:484761/1‑140 (MQ=255)                                                                                      tttCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAGACTCACTGGCGATGATTTTGGCGCTGCgg                                <  2:770058/140‑1 (MQ=255)                                                                                                         aTGCGCCGCCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCgg             >  1:1220815/1‑140 (MQ=255)                                                                                                            cgccgcCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGa          <  2:1023496/140‑1 (MQ=255)                                                                                                            cgccgcCACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGa          >  1:1023496/1‑140 (MQ=255)                                                                                                                 ccACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCgg             >  1:95781/1‑132 (MQ=255)                                                                                                                 ccACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCgg             <  2:95781/132‑1 (MQ=255)                                                                                                                 ccACTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGACAGAt     >  2:1007311/1‑140 (MQ=255)                                                                                                                    cTTTCTTGCCATCGCCTTTGGTCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGACAGATTTc  >  1:341258/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                       GTTCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGCCTGTAACAAGAATGCGTGATCGTAACCTTTCACTTTGCGCTGATCGTCGTCGGCAAGAAACTCACTGGCGATGATTTTGGCGCTGCGGAAATCAAAAGACGTTCCGGCGACAGATTTC  >  NC_000913/787948‑788202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑