Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	980,658	A→G	T1445A (ACC→GCC)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	980,658	0	A	G	100.0%	141.5 / NA	39	T1445A (ACC→GCC)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

TTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTA  >  NC_000913/980522‑980791                                                                                                                                         |                                                                                                                                      ttGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCt                                                                                                                                    <  1:974029/140‑1 (MQ=255)        ctgctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACt                                                                                                                             >  1:999866/1‑140 (MQ=255)        ctgctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACt                                                                                                                             >  1:1126998/1‑140 (MQ=255)         tgctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTg                                                                                                                            <  2:746481/140‑1 (MQ=255)         tgctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAAAAAGGCACCGCCTATAAACTg                                                                                                                            <  1:187977/140‑1 (MQ=255)          gctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGAAAATGCACCTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTgg                                                                                                                           <  1:493458/140‑1 (MQ=255)                   cGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAg                                                                                                                  <  2:999866/140‑1 (MQ=255)                   cGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAg                                                                                                                  <  2:1126998/140‑1 (MQ=255)                        aaGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAg                                                                                                                  >  1:105596/1‑135 (MQ=255)                        aaGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAg                                                                                                                  <  2:105596/135‑1 (MQ=255)                               gcgACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATa                                                                                                      <  2:426448/140‑1 (MQ=255)                                           tCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATAc                                                                                                     >  1:919990/1‑129 (MQ=255)                                           tCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATAc                                                                                                     <  2:919990/129‑1 (MQ=255)                                            cGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGAAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCa                                                                                         <  2:379077/140‑1 (MQ=255)                                                cTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGa                                                                                                  <  1:768586/127‑1 (MQ=255)                                                cTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGa                                                                                                  >  2:768586/1‑127 (MQ=255)                                                      ccACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGc                                                                               <  2:1113278/140‑1 (MQ=255)                                                         cGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGtcgtcg                                                                                 <  2:1085610/135‑1 (MQ=255)                                                         cGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGtcgtcg                                                                                 >  1:1085610/1‑135 (MQ=255)                                                         cGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTg                                                                            >  1:959413/1‑140 (MQ=255)                                                         cGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGCGCGTAAAGTCTTCCAGAATACCGAACACGTTCAAGTCGTCGGCCTg                                                                            >  2:5534/1‑140 (MQ=255)                                                              tttGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAgg                                                                       >  2:600779/1‑140 (MQ=255)                                                                     tgtgtgAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTgcg                                                                <  1:600779/140‑1 (MQ=255)                                                                        gtgAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGcgccg                                                             >  1:933388/1‑140 (MQ=255)                                                                        gtgAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGcgccg                                                             >  2:834484/1‑140 (MQ=255)                                                                                    aaaTGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGaa                                                 <  1:834484/140‑1 (MQ=255)                                                                                         cAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCt                                            >  2:365014/1‑140 (MQ=255)                                                                                           aCTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTc                                          >  2:645498/1‑140 (MQ=255)                                                                                            cTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTcc                                         <  2:959413/140‑1 (MQ=255)                                                                                             tcatcaTCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTAGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCa                                        >  2:1143532/1‑140 (MQ=255)                                                                                              catcatCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTGCCAg                                       >  1:1144884/1‑140 (MQ=255)                                                                                                  atcGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAAc                                   >  2:413740/1‑140 (MQ=255)                                                                                                      cAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGAc                               >  2:574212/1‑140 (MQ=255)                                                                                                         cgcCGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGaa                            >  1:131557/1‑140 (MQ=255)                                                                                                           ccGGAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAgc                          <  1:851120/140‑1 (MQ=255)                                                                                                             ggAAAATATCAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAgcgc                        <  1:1143532/140‑1 (MQ=255)                                                                                                                      cAGCCCGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAgg               <  2:131557/140‑1 (MQ=255)                                                                                                                           cGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGc              >  2:1090635/1‑136 (MQ=255)                                                                                                                           cGGAGAAAGGCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGc              <  1:1090635/136‑1 (MQ=255)                                                                                                                                   ggCACCGCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTa  >  1:892448/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTA  >  NC_000913/980522‑980791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑