Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,041,264	A→G	L216L (TTA→TTG)	appA →	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,041,264	0	A	G	100.0%	249.1 / NA	68	L216L (TTA→TTG)	appA	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/39);  total (29/39)

GAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTACCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGAT  >  NC_000913/1041128‑1041399                                                                                                                                         |                                                                                                                                        gaggGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCa                                                                                                                                      >  1:1031873/1‑140 (MQ=255) gaggGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCa                                                                                                                                      >  1:969244/1‑140 (MQ=255)  aggGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCAt                                                                                                                                     <  2:969244/140‑1 (MQ=255)   gggTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATc                                                                                                                                    <  2:1031873/140‑1 (MQ=255)   gggTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATc                                                                                                                                    <  2:1196402/140‑1 (MQ=255)     gTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCgg                                                                                                                                  <  2:885511/140‑1 (MQ=255)      tCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGa                                                                                                                                 >  1:442184/1‑140 (MQ=255)              tGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTTTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAgg                                                                                                                         <  2:1113127/140‑1 (MQ=255)              tGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAgg                                                                                                                         <  1:585429/140‑1 (MQ=255)                   ttACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAgc                                                                                                                    >  1:47844/1‑140 (MQ=255)                    tACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAgcg                                                                                                                   <  1:1079059/140‑1 (MQ=255)                     aCCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAgcgc                                                                                                                  >  1:1017256/1‑140 (MQ=255)                          gCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACa                                                                                                             <  2:442184/140‑1 (MQ=255)                             tCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATg                                                                                                          <  1:198283/140‑1 (MQ=255)                               ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGtc                                                                                                        <  2:1219234/140‑1 (MQ=255)                                gCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATg                                                                                                          >  1:331510/1‑137 (MQ=255)                                gCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATg                                                                                                          <  2:331510/137‑1 (MQ=255)                                gCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGtct                                                                                                       >  1:556189/1‑140 (MQ=255)                                       gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAAc                                                                                                <  1:376989/140‑1 (MQ=255)                                        cGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAAcc                                                                                               >  2:193681/1‑140 (MQ=255)                                           ttCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGt                                                                                            <  1:228353/140‑1 (MQ=255)                                            tCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTg                                                                                           <  1:303969/140‑1 (MQ=255)                                                 aaCTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTa                                                                                      <  1:206081/140‑1 (MQ=255)                                                   cTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAg                                                                                    <  1:323435/140‑1 (MQ=255)                                                   cTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAACGTCTCATTAACCGGTGCGGTAAg                                                                                    <  1:839928/140‑1 (MQ=255)                                                        aCGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTCAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCg                                                                               <  2:1135734/140‑1 (MQ=255)                                                              gCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCaa                                                                         >  2:1080094/1‑140 (MQ=255)                                                                      ttCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACg                                                                 >  1:464860/1‑140 (MQ=255)                                                                                  aCTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTtctc                                                     <  2:556189/140‑1 (MQ=255)                                                                                    ttGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCt                                                   <  1:1080094/140‑1 (MQ=255)                                                                                      gtgCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGga                                                               >  1:19939/1‑126 (MQ=255)                                                                                      gtgCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGga                                                               <  2:19939/126‑1 (MQ=255)                                                                                      gtgCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATAtt                                                         >  2:356759/1‑132 (MQ=255)                                                                                      gtgCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATAtt                                                         <  1:356759/132‑1 (MQ=255)                                                                                       tgCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGca                                                >  2:417043/1‑140 (MQ=255)                                                                                        gCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGcaa                                               <  1:930458/140‑1 (MQ=255)                                                                                         ccTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGcaac                                              <  2:834603/140‑1 (MQ=255)                                                                                            tAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGTGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAg                                           <  1:920120/140‑1 (MQ=255)                                                                                            tAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAg                                           >  2:775455/1‑140 (MQ=255)                                                                                             aaaCGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGc                                          <  2:47844/140‑1 (MQ=255)                                                                                                 gTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAg                                      >  2:848188/1‑140 (MQ=255)                                                                                                        aCAGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGc                               <  2:677450/140‑1 (MQ=255)                                                                                                          aGGACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCg                             <  2:1017256/140‑1 (MQ=255)                                                                                                            gACGAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGa                           >  2:65126/1‑140 (MQ=255)                                                                                                               gAAAGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCACCGACAATGTCTCATTAACCGGTGCTGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGcc                        <  2:1005801/140‑1 (MQ=255)                                                                                                                 aaGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGcac                                        >  2:80121/1‑122 (MQ=255)                                                                                                                 aaGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGcac                                        <  1:80121/122‑1 (MQ=255)                                                                                                                 aaGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCgg                      <  1:417043/140‑1 (MQ=255)                                                                                                                  aGCTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCggg                     <  1:65126/140‑1 (MQ=255)                                                                                                                    cTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCggg                     <  1:390780/138‑1 (MQ=255)                                                                                                                    cTGTTCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCggg                     >  2:390780/1‑138 (MQ=255)                                                                                                                       ttCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTggg                >  1:1083275/1‑140 (MQ=255)                                                                                                                       ttCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTggg                >  2:280318/1‑140 (MQ=255)                                                                                                                       ttCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTggg                >  2:1064528/1‑140 (MQ=255)                                                                                                                       ttCATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTggg                >  1:94128/1‑140 (MQ=255)                                                                                                                         cATTAACGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGa              >  2:741835/1‑140 (MQ=255)                                                                                                                              aCGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGcc                        >  1:849921/1‑125 (MQ=255)                                                                                                                              aCGCAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGcc                        <  2:849921/125‑1 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCgtg                     <  2:424360/125‑3 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCggg                     >  1:424360/1‑125 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCAc      >  2:386681/1‑140 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCAc      <  1:501336/140‑1 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCAc      <  1:741835/140‑1 (MQ=255)                                                                                                                                 cAGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCAc      <  1:1064528/140‑1 (MQ=255)                                                                                                                                  aGGCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCAcc     >  2:371571/1‑140 (MQ=255)                                                                                                                                    gCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGa   >  2:501702/1‑140 (MQ=255)                                                                                                                                    gCATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGa   >  2:321889/1‑140 (MQ=255)                                                                                                                                     cATTGCCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGAt  <  1:371571/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCATTACCATCGGAACTCAAGGTGAGCGCCGACAATGTCTCATTAACCGGTGCGGTAAGCCTCGCATCAATGCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGAT  >  NC_000913/1041128‑1041399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑