Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,102,607	T→C	K197R (AAG→AGG)	csgD ←	DNA‑binding transcriptional dual regulator CsgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,102,607	0	T	C	100.0%	237.3 / NA	65	K197R (AAG→AGG)	csgD	DNA‑binding transcriptional dual regulator CsgD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/39);  total (26/39)

CAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGATGAGTAAGGAGGGCTGAT  >  NC_000913/1102472‑1102741                                                                                                                                        |                                                                                                                                       cAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATcctctt                                                                                                                                    >  1:161548/1‑140 (MQ=255) cAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATcctctt                                                                                                                                    >  1:39443/1‑140 (MQ=255)   aCGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGa                                                                                                                                  <  2:39443/140‑1 (MQ=255)   aCGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGa                                                                                                                                  >  1:199520/1‑140 (MQ=255)   aCGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGa                                                                                                                                  <  1:82303/140‑1 (MQ=255)    cGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaa                                                                                                                                 <  2:230795/140‑1 (MQ=255)     ggCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaa                                                                                                                                <  2:161548/140‑1 (MQ=255)     ggCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaa                                                                                                                                <  1:981668/140‑1 (MQ=255)     ggCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaa                                                                                                                                <  1:65641/140‑1 (MQ=255)      gCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaaa                                                                                                                               >  2:630926/1‑140 (MQ=255)      gCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaaa                                                                                                                               >  2:1154397/1‑140 (MQ=255)      gCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaaaa                                                                                                                               >  1:22840/1‑140 (MQ=255)        gTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGGTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGa                                                                                                                             >  2:579272/1‑140 (MQ=255)         tGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGAt                                                                                                                            >  1:1092380/1‑140 (MQ=255)             gATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATtata                                                                                                                        <  2:1065500/140‑1 (MQ=255)                tCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaa                                                                                                                                 >  1:42596/1‑128 (MQ=255)                tCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGaa                                                                                                                                 <  2:42596/128‑1 (MQ=255)                 cccTGCGGCGAACAGAAATTCTGTCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGa                                                                                                                    >  2:78617/1‑140 (MQ=255)                   cTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATg                                                                                                                  <  2:1168937/140‑1 (MQ=255)                   cTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATg                                                                                                                  >  1:141189/1‑140 (MQ=255)                     gcggcgAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCg                                                                                                                >  1:1052209/1‑140 (MQ=255)                         cgAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGtttt                                                                                                            >  2:932835/1‑140 (MQ=255)                               gAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGt                                                                                                      <  1:1154397/140‑1 (MQ=255)                                    tCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTAtttt                                                                                                 <  2:1208249/140‑1 (MQ=255)                                    tCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTAtttt                                                                                                 <  2:141189/140‑1 (MQ=255)                                    tCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTAtttt                                                                                                 <  2:1043932/140‑1 (MQ=255)                                      tGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTCGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCg                                                                                               <  2:1076960/140‑1 (MQ=255)                                          gccACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCtga                                                                                           >  1:125690/1‑140 (MQ=255)                                                     gCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTTTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACaacgaa                                                                                >  1:400368/1‑140 (MQ=255)                                                      cGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACaacgaac                                                                               >  1:713093/1‑140 (MQ=255)                                                           aTAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCg                                                                          <  1:227253/140‑1 (MQ=255)                                                             aaCGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGAt                                                                        <  1:78617/140‑1 (MQ=255)                                                              aCGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGAtc                                                                       <  2:22840/140‑1 (MQ=255)                                                               cGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGAtct                                                                      >  2:1107460/1‑140 (MQ=255)                                                                    cATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGAt                                                                        >  1:138230/1‑133 (MQ=255)                                                                    cATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGAt                                                                        <  2:138230/133‑1 (MQ=255)                                                                    cATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGtta                                                                 >  1:751204/1‑140 (MQ=255)                                                                           ttATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTTTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGAcg                                                          <  2:400368/140‑1 (MQ=255)                                                                           ttATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGAcg                                                          <  1:853974/140‑1 (MQ=255)                                                                            tATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCg                                                                          <  2:656134/123‑1 (MQ=255)                                                                            tATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCg                                                                          >  1:656134/1‑123 (MQ=255)                                                                            tATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGAcgc                                                         >  2:198877/1‑140 (MQ=255)                                                                            tATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGAcgc                                                         >  2:975624/1‑140 (MQ=255)                                                                             aTCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGAcgcg                                                        <  1:829997/140‑1 (MQ=255)                                                                              tCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACgcgc                                                       <  1:198877/140‑1 (MQ=255)                                                                              tCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACgcgc                                                       >  1:836063/1‑140 (MQ=255)                                                                                    gAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATAc                                                 <  2:713093/140‑1 (MQ=255)                                                                                         tATCGTTTGCCCAGGAAACCGCTTGTGTCCAGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGc                                            <  2:751204/140‑1 (MQ=255)                                                                                            cGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCATCTTa                                         <  2:1151657/140‑1 (MQ=255)                                                                                               ttGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATAc                                                 >  2:705546/1‑129 (MQ=255)                                                                                               ttGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATAc                                                 <  1:705546/129‑1 (MQ=255)                                                                                                tGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCa                                     >  1:422898/1‑140 (MQ=255)                                                                                                   ccAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGa                                  <  1:334340/140‑1 (MQ=255)                                                                                                   ccAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGa                                  <  1:340902/140‑1 (MQ=255)                                                                                                       gAAACCGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCATATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGAtctc                              >  1:1112651/1‑140 (MQ=255)                                                                                                            cGCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCttttt                         >  2:539544/1‑140 (MQ=255)                                                                                                             gCTTGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTc                        <  2:1119448/140‑1 (MQ=255)                                                                                                               ttGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACATCGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTccc                      <  2:1092380/140‑1 (MQ=255)                                                                                                               ttGTGTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTGCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTccc                      <  1:751502/140‑1 (MQ=255)                                                                                                                tgtgTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCg                     <  2:435109/140‑1 (MQ=255)                                                                                                                tgtgTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCg                     <  2:528509/140‑1 (MQ=255)                                                                                                                tgtgTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCg                     <  2:528510/140‑1 (MQ=255)                                                                                                                tgtgTCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCATATTTTCGCTGATGCACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCg                     <  2:1112651/140‑1 (MQ=255)                                                                                                                  tgtCCGGTTTTTTACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGAt                   <  2:422898/140‑1 (MQ=255)                                                                                                                          tttttACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGATGAGTAagg           <  2:400266/140‑1 (MQ=255)                                                                                                                          tttttACGGCTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGATGAGTAagg           <  1:539544/140‑1 (MQ=255)                                                                                                                                   cTATCCTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGATGAGTAAGGAGGGCTGAt  >  1:901576/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTTTCATGGCTTTATCGCCTGAGGTTATCGTTTGCCCAGGAAACCGCTTGTGTCCGGTTTTTTACGGCTATCTTCTTGAAAAGATTATAAAGATGCGTTTTAACCGTATTTTCGCTGATGAACAACGAACGAGCGATCTCGTTATTAGACGCGCCGATACGCAGCTTATTCAGGATCTCTTTTTCCCGATGAGTAAGGAGGGCTGAT  >  NC_000913/1102472‑1102741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑